Impact of cellular miRNAs on circulating miRNA biomarker signatures.

Effective diagnosis and surveillance of complex multi-factorial disorders such as cancer can be improved by screening of easily accessible biomarkers. Highly stable cell free Circulating Nucleic Acids (CNA) present as both RNA and DNA species have been discovered in the blood and plasma of humans. C...

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Autores principales:	Radha Duttagupta, Rong Jiang, Jeremy Gollub, Robert C Getts, Keith W Jones
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ae47bab95de345afb0c0f7e3de19e96d
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spelling	oai:doaj.org-article:ae47bab95de345afb0c0f7e3de19e96d2021-11-18T06:51:48ZImpact of cellular miRNAs on circulating miRNA biomarker signatures.1932-620310.1371/journal.pone.0020769https://doaj.org/article/ae47bab95de345afb0c0f7e3de19e96d2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21698099/?tool=EBIhttps://doaj.org/toc/1932-6203Effective diagnosis and surveillance of complex multi-factorial disorders such as cancer can be improved by screening of easily accessible biomarkers. Highly stable cell free Circulating Nucleic Acids (CNA) present as both RNA and DNA species have been discovered in the blood and plasma of humans. Correlations between tumor-associated genomic/epigenetic/transcriptional changes and alterations in CNA levels are strong predictors of the utility of this biomarker class as promising clinical indicators. Towards this goal microRNAs (miRNAs) representing a class of naturally occurring small non-coding RNAs of 19-25 nt in length have emerged as an important set of markers that can associate their specific expression profiles with cancer development. In this study we investigate some of the pre-analytic considerations for isolating plasma fractions for the study of miRNA biomarkers. We find that measurement of circulating miRNA levels are frequently confounded by varying levels of cellular miRNAs of different hematopoietic origins. In order to assess the relative proportions of this cell-derived class, we have fractionated whole blood into plasma and its ensuing sub-fractions. Cellular miRNA signatures in cohorts of normal individuals are catalogued and the abundance and gender specific expression of bona fide circulating markers explored after calibrating the signal for this interfering class. A map of differentially expressed profiles is presented and the intrinsic variability of circulating miRNA species investigated in subsets of healthy males and females.Radha DuttaguptaRong JiangJeremy GollubRobert C GettsKeith W JonesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e20769 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Radha Duttagupta Rong Jiang Jeremy Gollub Robert C Getts Keith W Jones Impact of cellular miRNAs on circulating miRNA biomarker signatures.
description	Effective diagnosis and surveillance of complex multi-factorial disorders such as cancer can be improved by screening of easily accessible biomarkers. Highly stable cell free Circulating Nucleic Acids (CNA) present as both RNA and DNA species have been discovered in the blood and plasma of humans. Correlations between tumor-associated genomic/epigenetic/transcriptional changes and alterations in CNA levels are strong predictors of the utility of this biomarker class as promising clinical indicators. Towards this goal microRNAs (miRNAs) representing a class of naturally occurring small non-coding RNAs of 19-25 nt in length have emerged as an important set of markers that can associate their specific expression profiles with cancer development. In this study we investigate some of the pre-analytic considerations for isolating plasma fractions for the study of miRNA biomarkers. We find that measurement of circulating miRNA levels are frequently confounded by varying levels of cellular miRNAs of different hematopoietic origins. In order to assess the relative proportions of this cell-derived class, we have fractionated whole blood into plasma and its ensuing sub-fractions. Cellular miRNA signatures in cohorts of normal individuals are catalogued and the abundance and gender specific expression of bona fide circulating markers explored after calibrating the signal for this interfering class. A map of differentially expressed profiles is presented and the intrinsic variability of circulating miRNA species investigated in subsets of healthy males and females.
format	article
author	Radha Duttagupta Rong Jiang Jeremy Gollub Robert C Getts Keith W Jones
author_facet	Radha Duttagupta Rong Jiang Jeremy Gollub Robert C Getts Keith W Jones
author_sort	Radha Duttagupta
title	Impact of cellular miRNAs on circulating miRNA biomarker signatures.
title_short	Impact of cellular miRNAs on circulating miRNA biomarker signatures.
title_full	Impact of cellular miRNAs on circulating miRNA biomarker signatures.
title_fullStr	Impact of cellular miRNAs on circulating miRNA biomarker signatures.
title_full_unstemmed	Impact of cellular miRNAs on circulating miRNA biomarker signatures.
title_sort	impact of cellular mirnas on circulating mirna biomarker signatures.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/ae47bab95de345afb0c0f7e3de19e96d
work_keys_str_mv	AT radhaduttagupta impactofcellularmirnasoncirculatingmirnabiomarkersignatures AT rongjiang impactofcellularmirnasoncirculatingmirnabiomarkersignatures AT jeremygollub impactofcellularmirnasoncirculatingmirnabiomarkersignatures AT robertcgetts impactofcellularmirnasoncirculatingmirnabiomarkersignatures AT keithwjones impactofcellularmirnasoncirculatingmirnabiomarkersignatures
_version_	1718424314105036800

Impact of cellular miRNAs on circulating miRNA biomarker signatures.

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