Identification of Metabolic Signatures Linked to Anti-Inflammatory Effects of <named-content content-type="genus-species">Faecalibacterium prausnitzii</named-content>

ABSTRACT Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified on the basis of human clinical data. The mechanisms underlying its beneficial effects are still unknown. Gnotobiotic mice harboring F. prausnitzii (A2-165) and Escherichia coli (K-12 JM105) were subjected to...

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Autores principales:	Sylvie Miquel, Marion Leclerc, Rebeca Martin, Florian Chain, Marion Lenoir, Sébastien Raguideau, Sylvie Hudault, Chantal Bridonneau, Trent Northen, Benjamin Bowen, Luis G. Bermúdez-Humarán, Harry Sokol, Muriel Thomas, Philippe Langella
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ae4c5bf756ac4b46ad9342d59a580d7c
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spelling	oai:doaj.org-article:ae4c5bf756ac4b46ad9342d59a580d7c2021-11-15T15:41:33ZIdentification of Metabolic Signatures Linked to Anti-Inflammatory Effects of <named-content content-type="genus-species">Faecalibacterium prausnitzii</named-content>10.1128/mBio.00300-152150-7511https://doaj.org/article/ae4c5bf756ac4b46ad9342d59a580d7c2015-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00300-15https://doaj.org/toc/2150-7511ABSTRACT Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified on the basis of human clinical data. The mechanisms underlying its beneficial effects are still unknown. Gnotobiotic mice harboring F. prausnitzii (A2-165) and Escherichia coli (K-12 JM105) were subjected to 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis. The inflammatory colitis scores and a gas chromatography-time of flight (GC/TOF) mass spectrometry-based metabolomic profile were monitored in blood, ileum, cecum, colon, and feces in gnotobiotic mice. The potential anti-inflammatory metabolites were tested in vitro. We obtained stable E. coli and F. prausnitzii-diassociated mice in which E. coli primed the gastrointestinal tract (GIT), allowing a durable and stable establishment of F. prausnitzii. The disease activity index, histological scores, myeloperoxidase (MPO) activity, and serum cytokine levels were significantly lower in the presence of F. prausnitzii after TNBS challenge. The protective effect of F. prausnitzii against colitis was correlated to its implantation level and was linked to overrepresented metabolites along the GIT and in serum. Among 983 metabolites in GIT samples and serum, 279 were assigned to known chemical reactions. Some of them, belonging to the ammonia (α-ketoglutarate), osmoprotective (raffinose), and phenolic (including anti-inflammatory shikimic and salicylic acids) pathways, were associated with a protective effect of F. prausnitzii, and the functional link was established in vitro for salicylic acid. We show for the first time that F. prausnitzii is a highly active commensal bacterium involved in reduction of colitis through in vivo modulation of metabolites along the GIT and in the peripheral blood. IMPORTANCE Inflammatory bowel diseases (IBD) are characterized by low proportions of F. prausnitzii in the gut microbiome. This commensal bacterium exhibits anti-inflammatory effects through still unknown mechanisms. Stable monoassociated rodents are actually not a reproducible model to decipher F. prausnitzii protective effects. We propose a new gnotobiotic rodent model providing mechanistic clues. In this model, F. prausnitzii exhibits protective effects against an acute colitis and a protective metabolic profile is linked to its presence along the digestive tract. We identified a molecule, salicylic acid, directly involved in the protective effect of F. prausnitzii. Targeting its metabolic pathways could be an attractive therapeutic strategy in IBD.Sylvie MiquelMarion LeclercRebeca MartinFlorian ChainMarion LenoirSébastien RaguideauSylvie HudaultChantal BridonneauTrent NorthenBenjamin BowenLuis G. Bermúdez-HumaránHarry SokolMuriel ThomasPhilippe LangellaAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 2 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Sylvie Miquel Marion Leclerc Rebeca Martin Florian Chain Marion Lenoir Sébastien Raguideau Sylvie Hudault Chantal Bridonneau Trent Northen Benjamin Bowen Luis G. Bermúdez-Humarán Harry Sokol Muriel Thomas Philippe Langella Identification of Metabolic Signatures Linked to Anti-Inflammatory Effects of <named-content content-type="genus-species">Faecalibacterium prausnitzii</named-content>
description	ABSTRACT Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified on the basis of human clinical data. The mechanisms underlying its beneficial effects are still unknown. Gnotobiotic mice harboring F. prausnitzii (A2-165) and Escherichia coli (K-12 JM105) were subjected to 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis. The inflammatory colitis scores and a gas chromatography-time of flight (GC/TOF) mass spectrometry-based metabolomic profile were monitored in blood, ileum, cecum, colon, and feces in gnotobiotic mice. The potential anti-inflammatory metabolites were tested in vitro. We obtained stable E. coli and F. prausnitzii-diassociated mice in which E. coli primed the gastrointestinal tract (GIT), allowing a durable and stable establishment of F. prausnitzii. The disease activity index, histological scores, myeloperoxidase (MPO) activity, and serum cytokine levels were significantly lower in the presence of F. prausnitzii after TNBS challenge. The protective effect of F. prausnitzii against colitis was correlated to its implantation level and was linked to overrepresented metabolites along the GIT and in serum. Among 983 metabolites in GIT samples and serum, 279 were assigned to known chemical reactions. Some of them, belonging to the ammonia (α-ketoglutarate), osmoprotective (raffinose), and phenolic (including anti-inflammatory shikimic and salicylic acids) pathways, were associated with a protective effect of F. prausnitzii, and the functional link was established in vitro for salicylic acid. We show for the first time that F. prausnitzii is a highly active commensal bacterium involved in reduction of colitis through in vivo modulation of metabolites along the GIT and in the peripheral blood. IMPORTANCE Inflammatory bowel diseases (IBD) are characterized by low proportions of F. prausnitzii in the gut microbiome. This commensal bacterium exhibits anti-inflammatory effects through still unknown mechanisms. Stable monoassociated rodents are actually not a reproducible model to decipher F. prausnitzii protective effects. We propose a new gnotobiotic rodent model providing mechanistic clues. In this model, F. prausnitzii exhibits protective effects against an acute colitis and a protective metabolic profile is linked to its presence along the digestive tract. We identified a molecule, salicylic acid, directly involved in the protective effect of F. prausnitzii. Targeting its metabolic pathways could be an attractive therapeutic strategy in IBD.
format	article
author	Sylvie Miquel Marion Leclerc Rebeca Martin Florian Chain Marion Lenoir Sébastien Raguideau Sylvie Hudault Chantal Bridonneau Trent Northen Benjamin Bowen Luis G. Bermúdez-Humarán Harry Sokol Muriel Thomas Philippe Langella
author_facet	Sylvie Miquel Marion Leclerc Rebeca Martin Florian Chain Marion Lenoir Sébastien Raguideau Sylvie Hudault Chantal Bridonneau Trent Northen Benjamin Bowen Luis G. Bermúdez-Humarán Harry Sokol Muriel Thomas Philippe Langella
author_sort	Sylvie Miquel
title	Identification of Metabolic Signatures Linked to Anti-Inflammatory Effects of <named-content content-type="genus-species">Faecalibacterium prausnitzii</named-content>
title_short	Identification of Metabolic Signatures Linked to Anti-Inflammatory Effects of <named-content content-type="genus-species">Faecalibacterium prausnitzii</named-content>
title_full	Identification of Metabolic Signatures Linked to Anti-Inflammatory Effects of <named-content content-type="genus-species">Faecalibacterium prausnitzii</named-content>
title_fullStr	Identification of Metabolic Signatures Linked to Anti-Inflammatory Effects of <named-content content-type="genus-species">Faecalibacterium prausnitzii</named-content>
title_full_unstemmed	Identification of Metabolic Signatures Linked to Anti-Inflammatory Effects of <named-content content-type="genus-species">Faecalibacterium prausnitzii</named-content>
title_sort	identification of metabolic signatures linked to anti-inflammatory effects of <named-content content-type="genus-species">faecalibacterium prausnitzii</named-content>
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/ae4c5bf756ac4b46ad9342d59a580d7c
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Identification of Metabolic Signatures Linked to Anti-Inflammatory Effects of <named-content content-type="genus-species">Faecalibacterium prausnitzii</named-content>

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