Macrophage Membrane-Coated Nanoparticles Alleviate Hepatic Ischemia-Reperfusion Injury Caused by Orthotopic Liver Transplantation by Neutralizing Endotoxin
Zhibing Ou,1,* Hua Zhong,2,* Liang Zhang,2,3 Minghua Deng,2 Wenfeng Zhang,2 Jingyuan Wang,2 Huaguo Feng,2 Jianping Gong,2 Chunmu Miao,2 Zhujun Yi2 1Department of Hepatobiliary Surgery, Chenzhou No.1 People’s Hospital, Chenzhou, Hunan 410000, People’s Republic of China; 2Departmen...
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Dove Medical Press
2020
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oai:doaj.org-article:ae62e0958746420aa864b322c9c4c6122021-12-02T10:33:25ZMacrophage Membrane-Coated Nanoparticles Alleviate Hepatic Ischemia-Reperfusion Injury Caused by Orthotopic Liver Transplantation by Neutralizing Endotoxin1178-2013https://doaj.org/article/ae62e0958746420aa864b322c9c4c6122020-06-01T00:00:00Zhttps://www.dovepress.com/macrophage-membrane-coated-nanoparticles-alleviate-hepatic-ischemia-re-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhibing Ou,1,* Hua Zhong,2,* Liang Zhang,2,3 Minghua Deng,2 Wenfeng Zhang,2 Jingyuan Wang,2 Huaguo Feng,2 Jianping Gong,2 Chunmu Miao,2 Zhujun Yi2 1Department of Hepatobiliary Surgery, Chenzhou No.1 People’s Hospital, Chenzhou, Hunan 410000, People’s Republic of China; 2Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 40000, People’s Republic of China; 3Institute of Ultrasound Imaging, Department of Ultrasound, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 40000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhujun Yi; Chunmu MiaoDepartment of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 40000, People’s Republic of ChinaTel +86-15803630430Email 792937539@qq.com; luckmum@163.comPurpose: To investigate the effect and mechanism of macrophage membrane-coated nanoparticles (M-NPs) on hepatic ischemia-reperfusion injury (I/RI) caused by orthotopic liver transplantation. In addition, the advantages of TLR4+/M-NPs compared to M-NPs are discussed.Materials and Methods: We prepared biomimetic M-NPs and identified their characteristics. M-NPs were injected into an SD rat model of orthotopic liver transplantation, and the anti-inflammatory and anti-I/RI activities of M-NPs were studied in vivo and in vitro. In addition, we overexpressed macrophage membrane Toll-like receptor 4 (TLR4) in vitro and prepared TLR4+/M-NPs. Then, we assessed the characteristics and advantages of TLR4+/M-NPs.Results: The M-NPs neutralized endotoxin, inhibited the overactivation of Kupffer cells (KCs) and suppressed the secretion of inflammatory factors by inhibiting the endotoxin-mediated TLR4/MyD88/IRAK1/NF-κB signaling pathway. In an orthotopic liver transplantation model in SD rats, M-NPs showed significant therapeutic efficacy by neutralizing endotoxin and suppressing the secretion of inflammatory factors. Moreover, overexpression of TLR4 on the macrophage membrane by using a TLR4+-plasmid in vitro effectively reduced the amount of M-NPs needed to neutralize an equivalent dose of endotoxin, reducing the potential risks of NP overuse.Conclusion: This study indicates that M-NPs can effectively alleviate I/RI induced by liver transplantation.Keywords: biomimetic nanoparticle, endotoxin, Kupffer cell, liver transplantation, ischemia reperfusion injuryOu ZZhong HZhang LDeng MZhang WWang JFeng HGong JMiao CYi ZDove Medical Pressarticlebiomimetic nanoparticleendotoxinkupffer cellliver transplantationischemia reperfusion injuryMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 4125-4138 (2020) |
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biomimetic nanoparticle endotoxin kupffer cell liver transplantation ischemia reperfusion injury Medicine (General) R5-920 |
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biomimetic nanoparticle endotoxin kupffer cell liver transplantation ischemia reperfusion injury Medicine (General) R5-920 Ou Z Zhong H Zhang L Deng M Zhang W Wang J Feng H Gong J Miao C Yi Z Macrophage Membrane-Coated Nanoparticles Alleviate Hepatic Ischemia-Reperfusion Injury Caused by Orthotopic Liver Transplantation by Neutralizing Endotoxin |
| description |
Zhibing Ou,1,* Hua Zhong,2,* Liang Zhang,2,3 Minghua Deng,2 Wenfeng Zhang,2 Jingyuan Wang,2 Huaguo Feng,2 Jianping Gong,2 Chunmu Miao,2 Zhujun Yi2 1Department of Hepatobiliary Surgery, Chenzhou No.1 People’s Hospital, Chenzhou, Hunan 410000, People’s Republic of China; 2Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 40000, People’s Republic of China; 3Institute of Ultrasound Imaging, Department of Ultrasound, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 40000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhujun Yi; Chunmu MiaoDepartment of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 40000, People’s Republic of ChinaTel +86-15803630430Email 792937539@qq.com; luckmum@163.comPurpose: To investigate the effect and mechanism of macrophage membrane-coated nanoparticles (M-NPs) on hepatic ischemia-reperfusion injury (I/RI) caused by orthotopic liver transplantation. In addition, the advantages of TLR4+/M-NPs compared to M-NPs are discussed.Materials and Methods: We prepared biomimetic M-NPs and identified their characteristics. M-NPs were injected into an SD rat model of orthotopic liver transplantation, and the anti-inflammatory and anti-I/RI activities of M-NPs were studied in vivo and in vitro. In addition, we overexpressed macrophage membrane Toll-like receptor 4 (TLR4) in vitro and prepared TLR4+/M-NPs. Then, we assessed the characteristics and advantages of TLR4+/M-NPs.Results: The M-NPs neutralized endotoxin, inhibited the overactivation of Kupffer cells (KCs) and suppressed the secretion of inflammatory factors by inhibiting the endotoxin-mediated TLR4/MyD88/IRAK1/NF-κB signaling pathway. In an orthotopic liver transplantation model in SD rats, M-NPs showed significant therapeutic efficacy by neutralizing endotoxin and suppressing the secretion of inflammatory factors. Moreover, overexpression of TLR4 on the macrophage membrane by using a TLR4+-plasmid in vitro effectively reduced the amount of M-NPs needed to neutralize an equivalent dose of endotoxin, reducing the potential risks of NP overuse.Conclusion: This study indicates that M-NPs can effectively alleviate I/RI induced by liver transplantation.Keywords: biomimetic nanoparticle, endotoxin, Kupffer cell, liver transplantation, ischemia reperfusion injury |
| format |
article |
| author |
Ou Z Zhong H Zhang L Deng M Zhang W Wang J Feng H Gong J Miao C Yi Z |
| author_facet |
Ou Z Zhong H Zhang L Deng M Zhang W Wang J Feng H Gong J Miao C Yi Z |
| author_sort |
Ou Z |
| title |
Macrophage Membrane-Coated Nanoparticles Alleviate Hepatic Ischemia-Reperfusion Injury Caused by Orthotopic Liver Transplantation by Neutralizing Endotoxin |
| title_short |
Macrophage Membrane-Coated Nanoparticles Alleviate Hepatic Ischemia-Reperfusion Injury Caused by Orthotopic Liver Transplantation by Neutralizing Endotoxin |
| title_full |
Macrophage Membrane-Coated Nanoparticles Alleviate Hepatic Ischemia-Reperfusion Injury Caused by Orthotopic Liver Transplantation by Neutralizing Endotoxin |
| title_fullStr |
Macrophage Membrane-Coated Nanoparticles Alleviate Hepatic Ischemia-Reperfusion Injury Caused by Orthotopic Liver Transplantation by Neutralizing Endotoxin |
| title_full_unstemmed |
Macrophage Membrane-Coated Nanoparticles Alleviate Hepatic Ischemia-Reperfusion Injury Caused by Orthotopic Liver Transplantation by Neutralizing Endotoxin |
| title_sort |
macrophage membrane-coated nanoparticles alleviate hepatic ischemia-reperfusion injury caused by orthotopic liver transplantation by neutralizing endotoxin |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/ae62e0958746420aa864b322c9c4c612 |
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