Developmental and environmental regulation of Aquaporin gene expression across Populus species: divergence or redundancy?

Aquaporins (AQPs) are membrane channels belonging to the major intrinsic proteins family and are known for their ability to facilitate water movement. While in Populus trichocarpa, AQP proteins form a large family encompassing fifty-five genes, most of the experimental work focused on a few genes or...

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Autores principales:	David Cohen, Marie-Béatrice Bogeat-Triboulot, Silvère Vialet-Chabrand, Rémy Merret, Pierre-Emmanuel Courty, Sébastien Moretti, François Bizet, Agnès Guilliot, Irène Hummel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ae650576791b4e358f32fccb169e98cd
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spelling	oai:doaj.org-article:ae650576791b4e358f32fccb169e98cd2021-11-18T07:58:47ZDevelopmental and environmental regulation of Aquaporin gene expression across Populus species: divergence or redundancy?1932-620310.1371/journal.pone.0055506https://doaj.org/article/ae650576791b4e358f32fccb169e98cd2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23393587/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Aquaporins (AQPs) are membrane channels belonging to the major intrinsic proteins family and are known for their ability to facilitate water movement. While in Populus trichocarpa, AQP proteins form a large family encompassing fifty-five genes, most of the experimental work focused on a few genes or subfamilies. The current work was undertaken to develop a comprehensive picture of the whole AQP gene family in Populus species by delineating gene expression domain and distinguishing responsiveness to developmental and environmental cues. Since duplication events amplified the poplar AQP family, we addressed the question of expression redundancy between gene duplicates. On these purposes, we carried a meta-analysis of all publicly available Affymetrix experiments. Our in-silico strategy controlled for previously identified biases in cross-species transcriptomics, a necessary step for any comparative transcriptomics based on multispecies design chips. Three poplar AQPs were not supported by any expression data, even in a large collection of situations (abiotic and biotic constraints, temporal oscillations and mutants). The expression of 11 AQPs was never or poorly regulated whatever the wideness of their expression domain and their expression level. Our work highlighted that PtTIP1;4 was the most responsive gene of the AQP family. A high functional divergence between gene duplicates was detected across species and in response to tested cues, except for the root-expressed PtTIP2;3/PtTIP2;4 pair exhibiting 80% convergent responses. Our meta-analysis assessed key features of aquaporin expression which had remained hidden in single experiments, such as expression wideness, response specificity and genotype and environment interactions. By consolidating expression profiles using independent experimental series, we showed that the large expansion of AQP family in poplar was accompanied with a strong divergence of gene expression, even if some cases of functional redundancy could be suspected.David CohenMarie-Béatrice Bogeat-TriboulotSilvère Vialet-ChabrandRémy MerretPierre-Emmanuel CourtySébastien MorettiFrançois BizetAgnès GuilliotIrène HummelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55506 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Cohen Marie-Béatrice Bogeat-Triboulot Silvère Vialet-Chabrand Rémy Merret Pierre-Emmanuel Courty Sébastien Moretti François Bizet Agnès Guilliot Irène Hummel Developmental and environmental regulation of Aquaporin gene expression across Populus species: divergence or redundancy?
description	Aquaporins (AQPs) are membrane channels belonging to the major intrinsic proteins family and are known for their ability to facilitate water movement. While in Populus trichocarpa, AQP proteins form a large family encompassing fifty-five genes, most of the experimental work focused on a few genes or subfamilies. The current work was undertaken to develop a comprehensive picture of the whole AQP gene family in Populus species by delineating gene expression domain and distinguishing responsiveness to developmental and environmental cues. Since duplication events amplified the poplar AQP family, we addressed the question of expression redundancy between gene duplicates. On these purposes, we carried a meta-analysis of all publicly available Affymetrix experiments. Our in-silico strategy controlled for previously identified biases in cross-species transcriptomics, a necessary step for any comparative transcriptomics based on multispecies design chips. Three poplar AQPs were not supported by any expression data, even in a large collection of situations (abiotic and biotic constraints, temporal oscillations and mutants). The expression of 11 AQPs was never or poorly regulated whatever the wideness of their expression domain and their expression level. Our work highlighted that PtTIP1;4 was the most responsive gene of the AQP family. A high functional divergence between gene duplicates was detected across species and in response to tested cues, except for the root-expressed PtTIP2;3/PtTIP2;4 pair exhibiting 80% convergent responses. Our meta-analysis assessed key features of aquaporin expression which had remained hidden in single experiments, such as expression wideness, response specificity and genotype and environment interactions. By consolidating expression profiles using independent experimental series, we showed that the large expansion of AQP family in poplar was accompanied with a strong divergence of gene expression, even if some cases of functional redundancy could be suspected.
format	article
author	David Cohen Marie-Béatrice Bogeat-Triboulot Silvère Vialet-Chabrand Rémy Merret Pierre-Emmanuel Courty Sébastien Moretti François Bizet Agnès Guilliot Irène Hummel
author_facet	David Cohen Marie-Béatrice Bogeat-Triboulot Silvère Vialet-Chabrand Rémy Merret Pierre-Emmanuel Courty Sébastien Moretti François Bizet Agnès Guilliot Irène Hummel
author_sort	David Cohen
title	Developmental and environmental regulation of Aquaporin gene expression across Populus species: divergence or redundancy?
title_short	Developmental and environmental regulation of Aquaporin gene expression across Populus species: divergence or redundancy?
title_full	Developmental and environmental regulation of Aquaporin gene expression across Populus species: divergence or redundancy?
title_fullStr	Developmental and environmental regulation of Aquaporin gene expression across Populus species: divergence or redundancy?
title_full_unstemmed	Developmental and environmental regulation of Aquaporin gene expression across Populus species: divergence or redundancy?
title_sort	developmental and environmental regulation of aquaporin gene expression across populus species: divergence or redundancy?
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ae650576791b4e358f32fccb169e98cd
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Developmental and environmental regulation of Aquaporin gene expression across Populus species: divergence or redundancy?

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