Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes

Hamid Reza Samadikhah1,*, Asia Majidi2,*, Maryam Nikkhah2, Saman Hosseinkhani11Department of Biochemistry, 2Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran *These authors contributed equally to this work Purpose: Cationic liposomes (CLs) are...

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Autores principales:	Samadikhah HR, Majidi A, Nikkhah M, Hosseinkhani S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ae6682f00a404f7b9ac6d01ec3aecd35
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spelling	oai:doaj.org-article:ae6682f00a404f7b9ac6d01ec3aecd352021-12-02T05:49:42ZPreparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes1176-91141178-2013https://doaj.org/article/ae6682f00a404f7b9ac6d01ec3aecd352011-10-01T00:00:00Zhttp://www.dovepress.com/preparation-characterization-and-efficient-transfection-of-cationic-li-a8456https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hamid Reza Samadikhah1,, Asia Majidi2,, Maryam Nikkhah2, Saman Hosseinkhani11Department of Biochemistry, 2Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran *These authors contributed equally to this work Purpose: Cationic liposomes (CLs) are composed of phospholipid bilayers. One of the most important applications of these particles is in drug and gene delivery. However, using CLs to deliver therapeutic nucleic acids and drugs to target organs has some problems, including low transfection efficiency in vivo. The aim of this study was to develop novel CLs containing magnetite to overcome the deficiencies. Patients and methods: CLs and magnetic cationic liposomes (MCLs) were prepared using the freeze-dried empty liposome method. Luciferase-harboring vectors (pGL3) were transferred into liposomes and the transfection efficiencies were determined by luciferase assay. Firefly luciferase is one of most popular reporter genes often used to measure the efficiency of gene transfer in vivo and in vitro. Different formulations of liposomes have been used for delivery of different kinds of gene reporters. Lipoplex (liposome&ndash;plasmid DNA complexes) formation was monitored by gel retardation assay. Size and charge of lipoplexes were determined using particle size analysis. Chinese hamster ovary cells were transfected by lipoplexes (liposome-pGL3); transfection efficiency and gene expression level was evaluated by luciferase assay. Results: High transfection efficiency of plasmid by CLs and novel nanomagnetic CLs was achieved. Moreover, lipoplexes showed less cytotoxicity than polyethyleneimine and Lipofectamine&trade;. Conclusion: Novel liposome compositions (1,2-dipalmitoyl-sn-glycero-3-phosphocholine [DPPC]/dioctadecyldimethylammonium bromide [DOAB] and DPPC/cholesterol/DOAB) with high transfection efficiency can be useful in gene delivery in vitro. MCLs can also be used for targeted gene delivery, due to magnetic characteristic for conduction of genes or drugs to target organs. Keywords: transfection efficiency, magnetic nanoparticles, luciferase, cationic liposomeSamadikhah HRMajidi ANikkhah MHosseinkhani SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2275-2283 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Samadikhah HR Majidi A Nikkhah M Hosseinkhani S Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes
description	Hamid Reza Samadikhah1,, Asia Majidi2,, Maryam Nikkhah2, Saman Hosseinkhani11Department of Biochemistry, 2Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran *These authors contributed equally to this work Purpose: Cationic liposomes (CLs) are composed of phospholipid bilayers. One of the most important applications of these particles is in drug and gene delivery. However, using CLs to deliver therapeutic nucleic acids and drugs to target organs has some problems, including low transfection efficiency in vivo. The aim of this study was to develop novel CLs containing magnetite to overcome the deficiencies. Patients and methods: CLs and magnetic cationic liposomes (MCLs) were prepared using the freeze-dried empty liposome method. Luciferase-harboring vectors (pGL3) were transferred into liposomes and the transfection efficiencies were determined by luciferase assay. Firefly luciferase is one of most popular reporter genes often used to measure the efficiency of gene transfer in vivo and in vitro. Different formulations of liposomes have been used for delivery of different kinds of gene reporters. Lipoplex (liposome&ndash;plasmid DNA complexes) formation was monitored by gel retardation assay. Size and charge of lipoplexes were determined using particle size analysis. Chinese hamster ovary cells were transfected by lipoplexes (liposome-pGL3); transfection efficiency and gene expression level was evaluated by luciferase assay. Results: High transfection efficiency of plasmid by CLs and novel nanomagnetic CLs was achieved. Moreover, lipoplexes showed less cytotoxicity than polyethyleneimine and Lipofectamine&trade;. Conclusion: Novel liposome compositions (1,2-dipalmitoyl-sn-glycero-3-phosphocholine [DPPC]/dioctadecyldimethylammonium bromide [DOAB] and DPPC/cholesterol/DOAB) with high transfection efficiency can be useful in gene delivery in vitro. MCLs can also be used for targeted gene delivery, due to magnetic characteristic for conduction of genes or drugs to target organs. Keywords: transfection efficiency, magnetic nanoparticles, luciferase, cationic liposome
format	article
author	Samadikhah HR Majidi A Nikkhah M Hosseinkhani S
author_facet	Samadikhah HR Majidi A Nikkhah M Hosseinkhani S
author_sort	Samadikhah HR
title	Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes
title_short	Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes
title_full	Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes
title_fullStr	Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes
title_full_unstemmed	Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes
title_sort	preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/ae6682f00a404f7b9ac6d01ec3aecd35
work_keys_str_mv	AT samadikhahhr preparationcharacterizationandefficienttransfectionofcationicliposomesandnanomagneticcationicliposomes AT majidia preparationcharacterizationandefficienttransfectionofcationicliposomesandnanomagneticcationicliposomes AT nikkhahm preparationcharacterizationandefficienttransfectionofcationicliposomesandnanomagneticcationicliposomes AT hosseinkhanis preparationcharacterizationandefficienttransfectionofcationicliposomesandnanomagneticcationicliposomes
_version_	1718400251498332160

Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes

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