ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.

Arfs and Arf GTPase-activating proteins (ArfGAPs) are regulators of membrane trafficking and actin dynamics in mammalian cells. In this study, we identified a primordial Arf, ArfA, and two ArfGAPs (ACAP-A/B) containing BAR, PH, ArfGAP and Ankyrin repeat domains in the eukaryote Dictyostelium discoid...

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Autores principales:	Pei-Wen Chen, Paul A Randazzo, Carole A Parent
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ae69f76c0e6d420ca2475098a7c2a175
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spelling	oai:doaj.org-article:ae69f76c0e6d420ca2475098a7c2a1752021-11-25T06:26:53ZACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.1932-620310.1371/journal.pone.0008624https://doaj.org/article/ae69f76c0e6d420ca2475098a7c2a1752010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20062541/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Arfs and Arf GTPase-activating proteins (ArfGAPs) are regulators of membrane trafficking and actin dynamics in mammalian cells. In this study, we identified a primordial Arf, ArfA, and two ArfGAPs (ACAP-A/B) containing BAR, PH, ArfGAP and Ankyrin repeat domains in the eukaryote Dictyostelium discoideum. In vitro, ArfA has similar nucleotide binding properties as mammalian Arfs and, with GTP bound, is a substrate for ACAP-A and B. We also investigated the physiological functions of ACAP-A/B by characterizing cells lacking both ACAP-A and B. Although ACAP-A/B knockout cells showed no defects in cell growth, migration or chemotaxis, they exhibited abnormal actin protrusions and approximately 50% reduction in spore yield. We conclude that while ACAP-A/B have a conserved biochemical mechanism and effect on actin organization, their role in migration is not conserved. The absence of an effect on Dictyostelium migration may be due to a specific requirement for ACAPs in mesenchymal migration, which is observed in epithelial cancer cells where most studies of mammalian ArfGAPs were performed.Pei-Wen ChenPaul A RandazzoCarole A ParentPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8624 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pei-Wen Chen Paul A Randazzo Carole A Parent ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.
description	Arfs and Arf GTPase-activating proteins (ArfGAPs) are regulators of membrane trafficking and actin dynamics in mammalian cells. In this study, we identified a primordial Arf, ArfA, and two ArfGAPs (ACAP-A/B) containing BAR, PH, ArfGAP and Ankyrin repeat domains in the eukaryote Dictyostelium discoideum. In vitro, ArfA has similar nucleotide binding properties as mammalian Arfs and, with GTP bound, is a substrate for ACAP-A and B. We also investigated the physiological functions of ACAP-A/B by characterizing cells lacking both ACAP-A and B. Although ACAP-A/B knockout cells showed no defects in cell growth, migration or chemotaxis, they exhibited abnormal actin protrusions and approximately 50% reduction in spore yield. We conclude that while ACAP-A/B have a conserved biochemical mechanism and effect on actin organization, their role in migration is not conserved. The absence of an effect on Dictyostelium migration may be due to a specific requirement for ACAPs in mesenchymal migration, which is observed in epithelial cancer cells where most studies of mammalian ArfGAPs were performed.
format	article
author	Pei-Wen Chen Paul A Randazzo Carole A Parent
author_facet	Pei-Wen Chen Paul A Randazzo Carole A Parent
author_sort	Pei-Wen Chen
title	ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.
title_short	ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.
title_full	ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.
title_fullStr	ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.
title_full_unstemmed	ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.
title_sort	acap-a/b are arfgap homologs in dictyostelium involved in sporulation but not in chemotaxis.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/ae69f76c0e6d420ca2475098a7c2a175
work_keys_str_mv	AT peiwenchen acapabarearfgaphomologsindictyosteliuminvolvedinsporulationbutnotinchemotaxis AT paularandazzo acapabarearfgaphomologsindictyosteliuminvolvedinsporulationbutnotinchemotaxis AT caroleaparent acapabarearfgaphomologsindictyosteliuminvolvedinsporulationbutnotinchemotaxis
_version_	1718413702398476288

ACAP-A/B are ArfGAP homologs in dictyostelium involved in sporulation but not in chemotaxis.

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