Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array

In the past years, Esomeprazole (EMP) was analyzed in human plasma samples, which still has stability issues; thus, the new biosampling technique known as Dried Blood Spot (DBS) might solve the issue. This research aims to evaluate the incurred sample stability of esomeprazole in dried blood spot us...

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Autores principales:	Yahdiana Harahap, Anja Tamabri, Vicha Vicha, Herman Suryadi, Sunarsih Sunarsih, Callista Andinie Mulyadi
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2020
Materias:	dried blood spot esomeprazole lansoprazole hplc incurred sample Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/ae721bdad236431cbdbb0a69498bd03d
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spelling	oai:doaj.org-article:ae721bdad236431cbdbb0a69498bd03d2021-12-02T12:06:18ZPharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array1411-94202460-157810.22146/ijc.42404https://doaj.org/article/ae721bdad236431cbdbb0a69498bd03d2020-07-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/42404https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578In the past years, Esomeprazole (EMP) was analyzed in human plasma samples, which still has stability issues; thus, the new biosampling technique known as Dried Blood Spot (DBS) might solve the issue. This research aims to evaluate the incurred sample stability of esomeprazole in dried blood spot using high performance liquid chromatography-photodiode array with lansoprazole as an internal standard. The analytical separation was performed on a C-18 column (Waters, Sunfire™ 5 μm; 250 × 4.6 mm) at 40 °C. The mobile phase used was acetonitrile–phosphate buffer pH 7.6 (40:60% v/v) with a flow rate of 1.00 mL/min; and was detected at 300 nm. The analyte was extracted from dried blood spot by methanol. Incurred sample stability was evaluated from 6 healthy subjects on day 0, 7, 14, and 28, respectively. This method was linear in the range concentration of 70–1400 ng/mL with r > 0.98. Pharmacokinetic study shows that the average of AUC0–t of EMP in the DBS sample was 1765.41 ngh/mL. The highest percent difference value of esomeprazole’s incurred samples stability on day 7, 14, and 28 from 6 healthy subjects were 9.81%. This result fulfilled the acceptance criteria, which is the percent difference should not be greater than 20%, and 67% of total samples have to fulfill the criteria. The incurred sample stability result showed that esomeprazole was stable in the DBS sample at least until 28 days with the highest value of percent difference is 9.81%.Yahdiana HarahapAnja TamabriVicha VichaHerman SuryadiSunarsih SunarsihCallista Andinie MulyadiDepartment of Chemistry, Universitas Gadjah Madaarticledried blood spotesomeprazolelansoprazolehplcincurred sampleChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 20, Iss 5, Pp 979-986 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	dried blood spot esomeprazole lansoprazole hplc incurred sample Chemistry QD1-999
spellingShingle	dried blood spot esomeprazole lansoprazole hplc incurred sample Chemistry QD1-999 Yahdiana Harahap Anja Tamabri Vicha Vicha Herman Suryadi Sunarsih Sunarsih Callista Andinie Mulyadi Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array
description	In the past years, Esomeprazole (EMP) was analyzed in human plasma samples, which still has stability issues; thus, the new biosampling technique known as Dried Blood Spot (DBS) might solve the issue. This research aims to evaluate the incurred sample stability of esomeprazole in dried blood spot using high performance liquid chromatography-photodiode array with lansoprazole as an internal standard. The analytical separation was performed on a C-18 column (Waters, Sunfire™ 5 μm; 250 × 4.6 mm) at 40 °C. The mobile phase used was acetonitrile–phosphate buffer pH 7.6 (40:60% v/v) with a flow rate of 1.00 mL/min; and was detected at 300 nm. The analyte was extracted from dried blood spot by methanol. Incurred sample stability was evaluated from 6 healthy subjects on day 0, 7, 14, and 28, respectively. This method was linear in the range concentration of 70–1400 ng/mL with r > 0.98. Pharmacokinetic study shows that the average of AUC0–t of EMP in the DBS sample was 1765.41 ngh/mL. The highest percent difference value of esomeprazole’s incurred samples stability on day 7, 14, and 28 from 6 healthy subjects were 9.81%. This result fulfilled the acceptance criteria, which is the percent difference should not be greater than 20%, and 67% of total samples have to fulfill the criteria. The incurred sample stability result showed that esomeprazole was stable in the DBS sample at least until 28 days with the highest value of percent difference is 9.81%.
format	article
author	Yahdiana Harahap Anja Tamabri Vicha Vicha Herman Suryadi Sunarsih Sunarsih Callista Andinie Mulyadi
author_facet	Yahdiana Harahap Anja Tamabri Vicha Vicha Herman Suryadi Sunarsih Sunarsih Callista Andinie Mulyadi
author_sort	Yahdiana Harahap
title	Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array
title_short	Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array
title_full	Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array
title_fullStr	Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array
title_full_unstemmed	Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array
title_sort	pharmacokinetic study and incurred sample stability of esomeprazole in dried blood spot sample using high performance liquid chromatography-photodiode array
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/ae721bdad236431cbdbb0a69498bd03d
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Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array

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