Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo

Catarina Roma-Rodrigues,1 Amelie Heuer-Jungemann,2 Alexandra R Fernandes,1 Antonios G Kanaras,2 Pedro V Baptista1 1UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal; 2Institute for Life Sciences, Physics...

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Autores principales:	Roma-Rodrigues C, Heuer-Jungemann A, Fernandes AR, Kanaras AG, Baptista PV
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	angiogenesis activators anti-angiogenic CAM assay gold nanoparticles peptide-coated gold nanoparticles vascular development. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ae741a99287f4c66b15cd2995ab151d5
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spelling	oai:doaj.org-article:ae741a99287f4c66b15cd2995ab151d52021-12-02T01:46:44ZPeptide-coated gold nanoparticles for modulation of angiogenesis in vivo1178-2013https://doaj.org/article/ae741a99287f4c66b15cd2995ab151d52016-06-01T00:00:00Zhttps://www.dovepress.com/peptide-coated-gold-nanoparticles-for-modulation-of-angiogenesis-in-vi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Catarina Roma-Rodrigues,1 Amelie Heuer-Jungemann,2 Alexandra R Fernandes,1 Antonios G Kanaras,2 Pedro V Baptista1 1UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal; 2Institute for Life Sciences, Physics and Astronomy, Faculty of Physical Sciences and Engineering, University of Southampton, Southampton, UK Abstract: In this work, peptides designed to selectively interact with cellular receptors involved in the regulation of angiogenesis were anchored to oligo-ethylene glycol-capped gold nanoparticles (AuNPs) and used to evaluate the modulation of vascular development using an ex ovo chick chorioallantoic membrane assay. These nanoparticles alter the balance between naturally secreted pro- and antiangiogenic factors, under various biological conditions, without causing toxicity. Exposure of chorioallantoic membranes to AuNP–peptide activators of angiogenesis accelerated the formation of new arterioles when compared to scrambled peptide-coated nanoparticles. On the other hand, antiangiogenic AuNP–peptide conjugates were able to selectively inhibit angiogenesis in vivo. We demonstrated that AuNP vectorization is crucial for enhancing the effect of active peptides. Our data showed for the first time the effective control of activation or inhibition of blood vessel formation in chick embryo via AuNP-based formulations suitable for the selective modulation of angiogenesis, which is of paramount importance in applications where promotion of vascular growth is desirable (eg, wound healing) or ought to be contravened, as in cancer development. Keywords: angiogenesis activators, antiangiogenic, CAM assay, gold nanoparticles, peptide-coated gold nanoparticles, vascular developmentRoma-Rodrigues CHeuer-Jungemann AFernandes ARKanaras AGBaptista PVDove Medical Pressarticleangiogenesis activatorsanti-angiogenicCAM assaygold nanoparticlespeptide-coated gold nanoparticlesvascular development.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2633-2639 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	angiogenesis activators anti-angiogenic CAM assay gold nanoparticles peptide-coated gold nanoparticles vascular development. Medicine (General) R5-920
spellingShingle	angiogenesis activators anti-angiogenic CAM assay gold nanoparticles peptide-coated gold nanoparticles vascular development. Medicine (General) R5-920 Roma-Rodrigues C Heuer-Jungemann A Fernandes AR Kanaras AG Baptista PV Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo
description	Catarina Roma-Rodrigues,1 Amelie Heuer-Jungemann,2 Alexandra R Fernandes,1 Antonios G Kanaras,2 Pedro V Baptista1 1UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal; 2Institute for Life Sciences, Physics and Astronomy, Faculty of Physical Sciences and Engineering, University of Southampton, Southampton, UK Abstract: In this work, peptides designed to selectively interact with cellular receptors involved in the regulation of angiogenesis were anchored to oligo-ethylene glycol-capped gold nanoparticles (AuNPs) and used to evaluate the modulation of vascular development using an ex ovo chick chorioallantoic membrane assay. These nanoparticles alter the balance between naturally secreted pro- and antiangiogenic factors, under various biological conditions, without causing toxicity. Exposure of chorioallantoic membranes to AuNP–peptide activators of angiogenesis accelerated the formation of new arterioles when compared to scrambled peptide-coated nanoparticles. On the other hand, antiangiogenic AuNP–peptide conjugates were able to selectively inhibit angiogenesis in vivo. We demonstrated that AuNP vectorization is crucial for enhancing the effect of active peptides. Our data showed for the first time the effective control of activation or inhibition of blood vessel formation in chick embryo via AuNP-based formulations suitable for the selective modulation of angiogenesis, which is of paramount importance in applications where promotion of vascular growth is desirable (eg, wound healing) or ought to be contravened, as in cancer development. Keywords: angiogenesis activators, antiangiogenic, CAM assay, gold nanoparticles, peptide-coated gold nanoparticles, vascular development
format	article
author	Roma-Rodrigues C Heuer-Jungemann A Fernandes AR Kanaras AG Baptista PV
author_facet	Roma-Rodrigues C Heuer-Jungemann A Fernandes AR Kanaras AG Baptista PV
author_sort	Roma-Rodrigues C
title	Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo
title_short	Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo
title_full	Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo
title_fullStr	Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo
title_full_unstemmed	Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo
title_sort	peptide-coated gold nanoparticles for modulation of angiogenesis in vivo
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/ae741a99287f4c66b15cd2995ab151d5
work_keys_str_mv	AT romarodriguesc peptidecoatedgoldnanoparticlesformodulationofangiogenesisinvivo AT heuerjungemanna peptidecoatedgoldnanoparticlesformodulationofangiogenesisinvivo AT fernandesar peptidecoatedgoldnanoparticlesformodulationofangiogenesisinvivo AT kanarasag peptidecoatedgoldnanoparticlesformodulationofangiogenesisinvivo AT baptistapv peptidecoatedgoldnanoparticlesformodulationofangiogenesisinvivo
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Peptide-coated gold nanoparticles for modulation of angiogenesis in vivo

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