Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples

Abstract In biotechnology, medicine, and food processing, simple and reliable methods for cell membrane permeabilization are required for drug/gene delivery into the cells or for the inactivation of undesired microorganisms. Pulsed electric field treatment is among the most promising methods enablin...

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Autores principales:	Maša Kandušer, Aleš Belič, Selma Čorović, Igor Škrjanc
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ae7dc01e9f7c491cb18f25601675fbc1
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spelling	oai:doaj.org-article:ae7dc01e9f7c491cb18f25601675fbc12021-12-02T11:41:19ZModular Serial Flow Through device for pulsed electric field treatment of the liquid samples10.1038/s41598-017-08620-82045-2322https://doaj.org/article/ae7dc01e9f7c491cb18f25601675fbc12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08620-8https://doaj.org/toc/2045-2322Abstract In biotechnology, medicine, and food processing, simple and reliable methods for cell membrane permeabilization are required for drug/gene delivery into the cells or for the inactivation of undesired microorganisms. Pulsed electric field treatment is among the most promising methods enabling both aims. The drawback in current technology is controllable large volume operation. To address this challenge, we have developed an experimental setup for flow through electroporation with online regulation of the flow rate with feedback control. We have designed a modular serial flow-through co-linear chamber with a smooth inner surface, the uniform cross-section geometry through the majority of the system’s length, and the mesh in contact with the electrodes, which provides uniform electric field distribution and fluid velocity equilibration. The cylindrical cross-section of the chamber prevents arching at the active treatment region. We used mathematical modeling for the evaluation of electric field distribution and the flow profile in the active region. The system was tested for the inactivation of Escherichia coli. We compared two flow-through chambers and used a static chamber as a reference. The experiments were performed under identical experimental condition (product and similar process parameters). The data were analyzed in terms of inactivation efficiency and specific energy consumption.Maša KandušerAleš BeličSelma ČorovićIgor ŠkrjancNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maša Kandušer Aleš Belič Selma Čorović Igor Škrjanc Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples
description	Abstract In biotechnology, medicine, and food processing, simple and reliable methods for cell membrane permeabilization are required for drug/gene delivery into the cells or for the inactivation of undesired microorganisms. Pulsed electric field treatment is among the most promising methods enabling both aims. The drawback in current technology is controllable large volume operation. To address this challenge, we have developed an experimental setup for flow through electroporation with online regulation of the flow rate with feedback control. We have designed a modular serial flow-through co-linear chamber with a smooth inner surface, the uniform cross-section geometry through the majority of the system’s length, and the mesh in contact with the electrodes, which provides uniform electric field distribution and fluid velocity equilibration. The cylindrical cross-section of the chamber prevents arching at the active treatment region. We used mathematical modeling for the evaluation of electric field distribution and the flow profile in the active region. The system was tested for the inactivation of Escherichia coli. We compared two flow-through chambers and used a static chamber as a reference. The experiments were performed under identical experimental condition (product and similar process parameters). The data were analyzed in terms of inactivation efficiency and specific energy consumption.
format	article
author	Maša Kandušer Aleš Belič Selma Čorović Igor Škrjanc
author_facet	Maša Kandušer Aleš Belič Selma Čorović Igor Škrjanc
author_sort	Maša Kandušer
title	Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples
title_short	Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples
title_full	Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples
title_fullStr	Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples
title_full_unstemmed	Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples
title_sort	modular serial flow through device for pulsed electric field treatment of the liquid samples
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ae7dc01e9f7c491cb18f25601675fbc1
work_keys_str_mv	AT masakanduser modularserialflowthroughdeviceforpulsedelectricfieldtreatmentoftheliquidsamples AT alesbelic modularserialflowthroughdeviceforpulsedelectricfieldtreatmentoftheliquidsamples AT selmacorovic modularserialflowthroughdeviceforpulsedelectricfieldtreatmentoftheliquidsamples AT igorskrjanc modularserialflowthroughdeviceforpulsedelectricfieldtreatmentoftheliquidsamples
_version_	1718395419129544704

Modular Serial Flow Through device for pulsed electric field treatment of the liquid samples

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