Screening of long non-coding RNAs markers in plasma of children with chronic gastritis

Objective: The study aimed to detect and analyze long non-coding RNAs (lncRNAs) in plasma of children diagnosed with chronic gastritis, and to explore its biological functions and involved signaling pathways. Methods: The plasma samples were collected from six children that were diagnosed with chron...

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Autores principales:	Zhen Gu, Hua-Qin Shen, Pei-Hua Fu, Mei Chen
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2020
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ae81e6beb4b84adf8df2b29ac0bb9d35
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spelling	oai:doaj.org-article:ae81e6beb4b84adf8df2b29ac0bb9d352021-12-02T13:39:21ZScreening of long non-coding RNAs markers in plasma of children with chronic gastritis2095-882X10.1016/j.cdtm.2020.01.001https://doaj.org/article/ae81e6beb4b84adf8df2b29ac0bb9d352020-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2095882X20300013https://doaj.org/toc/2095-882XObjective: The study aimed to detect and analyze long non-coding RNAs (lncRNAs) in plasma of children diagnosed with chronic gastritis, and to explore its biological functions and involved signaling pathways. Methods: The plasma samples were collected from six children that were diagnosed with chronic gastritis by physical examination, gastroscopy, and pathological examination and six healthy children. The plasma samples were assayed for determining the expression profiles of lncRNA based upon the gen chip detection. The specific expression of lcnRNA in plasma of children with chronic gastritis was analyzed and its biological functions were speculated. Results: Five lncRNAs (RP11-697M17.1, RP11-388M20.9, AFAP1-AS1, BC062758, and XLOC001406) were significantly up-regulated, and five lncRNAs (UNQ697, BX571672.5, CYP4F35P, ANKRD20A5P, and AL832737) were observed to be significantly down-regulated. The lncRNAs RP11-697M17.1, and UNQ697 were detected with the highest up-regulation and down-regulation, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the up-regulated lncRNAs were significantly enriched in 20 signaling pathways such as phosphoinositide-3-kinase–protein kinase B (PI3K-Akt) pathway, and the down-regulated lncRNAs target genes were significantly enriched in 20 signaling pathways such as the metabolic pathway. Conclusion: The analysis of the lncRNA expression profiles in plasma of children with chronic gastritis revealed that the lncRNA RP11-697M17.1, and lncRNA UNQ697 may act as plasma markers for predicting chronic gastritis in children. Keywords: RP11-697M17.1, UNQ697, Chronic gastritis, Plasma markersZhen GuHua-Qin ShenPei-Hua FuMei ChenKeAi Communications Co., Ltd.articleMedicine (General)R5-920ENChronic Diseases and Translational Medicine, Vol 6, Iss 1, Pp 62-68 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhen Gu Hua-Qin Shen Pei-Hua Fu Mei Chen Screening of long non-coding RNAs markers in plasma of children with chronic gastritis
description	Objective: The study aimed to detect and analyze long non-coding RNAs (lncRNAs) in plasma of children diagnosed with chronic gastritis, and to explore its biological functions and involved signaling pathways. Methods: The plasma samples were collected from six children that were diagnosed with chronic gastritis by physical examination, gastroscopy, and pathological examination and six healthy children. The plasma samples were assayed for determining the expression profiles of lncRNA based upon the gen chip detection. The specific expression of lcnRNA in plasma of children with chronic gastritis was analyzed and its biological functions were speculated. Results: Five lncRNAs (RP11-697M17.1, RP11-388M20.9, AFAP1-AS1, BC062758, and XLOC001406) were significantly up-regulated, and five lncRNAs (UNQ697, BX571672.5, CYP4F35P, ANKRD20A5P, and AL832737) were observed to be significantly down-regulated. The lncRNAs RP11-697M17.1, and UNQ697 were detected with the highest up-regulation and down-regulation, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that the up-regulated lncRNAs were significantly enriched in 20 signaling pathways such as phosphoinositide-3-kinase–protein kinase B (PI3K-Akt) pathway, and the down-regulated lncRNAs target genes were significantly enriched in 20 signaling pathways such as the metabolic pathway. Conclusion: The analysis of the lncRNA expression profiles in plasma of children with chronic gastritis revealed that the lncRNA RP11-697M17.1, and lncRNA UNQ697 may act as plasma markers for predicting chronic gastritis in children. Keywords: RP11-697M17.1, UNQ697, Chronic gastritis, Plasma markers
format	article
author	Zhen Gu Hua-Qin Shen Pei-Hua Fu Mei Chen
author_facet	Zhen Gu Hua-Qin Shen Pei-Hua Fu Mei Chen
author_sort	Zhen Gu
title	Screening of long non-coding RNAs markers in plasma of children with chronic gastritis
title_short	Screening of long non-coding RNAs markers in plasma of children with chronic gastritis
title_full	Screening of long non-coding RNAs markers in plasma of children with chronic gastritis
title_fullStr	Screening of long non-coding RNAs markers in plasma of children with chronic gastritis
title_full_unstemmed	Screening of long non-coding RNAs markers in plasma of children with chronic gastritis
title_sort	screening of long non-coding rnas markers in plasma of children with chronic gastritis
publisher	KeAi Communications Co., Ltd.
publishDate	2020
url	https://doaj.org/article/ae81e6beb4b84adf8df2b29ac0bb9d35
work_keys_str_mv	AT zhengu screeningoflongnoncodingrnasmarkersinplasmaofchildrenwithchronicgastritis AT huaqinshen screeningoflongnoncodingrnasmarkersinplasmaofchildrenwithchronicgastritis AT peihuafu screeningoflongnoncodingrnasmarkersinplasmaofchildrenwithchronicgastritis AT meichen screeningoflongnoncodingrnasmarkersinplasmaofchildrenwithchronicgastritis
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Screening of long non-coding RNAs markers in plasma of children with chronic gastritis

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