Validation of a Feed Protocol in a Mouse Model That Mimics Marasmic Malnutrition

Host nutritional status directly interferes with immunity and/or susceptibility to infectious diseases. To understand the mechanisms behind this relationship, the use of animal models and feeding protocols is necessary. In the literature, studies reporting marasmic malnutrition in mice are not commo...

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Autores principales:	Taiana Ferreira-Paes, Paula Seixas-Costa, Elmo Eduardo Almeida-Amaral
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	malnutrition refeeding mice phenotypic markers marasmic malnutrition Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/ae881053145e4671adcdce9ffc4ef3fd
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spelling	oai:doaj.org-article:ae881053145e4671adcdce9ffc4ef3fd2021-12-01T13:08:00ZValidation of a Feed Protocol in a Mouse Model That Mimics Marasmic Malnutrition2297-176910.3389/fvets.2021.757136https://doaj.org/article/ae881053145e4671adcdce9ffc4ef3fd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.757136/fullhttps://doaj.org/toc/2297-1769Host nutritional status directly interferes with immunity and/or susceptibility to infectious diseases. To understand the mechanisms behind this relationship, the use of animal models and feeding protocols is necessary. In the literature, studies reporting marasmic malnutrition in mice are not common. In this context, the objective of this study was to validate a feed methodology that mimics marasmic malnutrition, examining the nutritional, biochemical, and hematological status in BALB/c mice. Weaned BALB/c mice were or were not fed a Restricted diet (36.26% carbohydrate, 8.79% protein, 4.95% fat, and 7.62 kJ/100 g). Some malnourished mice underwent a refed process with a Control diet (65.93% carbohydrate, 24.18% protein, 9.89% fat, and 15.24 kJ/100 g). The nutritional status of the mice was evaluated through phenotypic markers and hematological and biochemical parameters. Our results showed that the Restricted diet was able to induce mild malnutrition in mice, resulting in mouse weight loss of 12%, which could be reversed after refeeding. Malnourished mice demonstrated slow body growth and low body mass index (BMI) values. Malnourished mice also showed physical and behavioral changes, a reduction of 47.5% in leukocyte counts and a 2-fold increase in cholesterol levels. In conclusion, our feeding protocol was able to generate mild malnutrition and cause changes in the nutritional status of mice that could be similar to those observed in marasmic malnutrition.Taiana Ferreira-PaesPaula Seixas-CostaElmo Eduardo Almeida-AmaralFrontiers Media S.A.articlemalnutritionrefeedingmicephenotypic markersmarasmic malnutritionVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	malnutrition refeeding mice phenotypic markers marasmic malnutrition Veterinary medicine SF600-1100
spellingShingle	malnutrition refeeding mice phenotypic markers marasmic malnutrition Veterinary medicine SF600-1100 Taiana Ferreira-Paes Paula Seixas-Costa Elmo Eduardo Almeida-Amaral Validation of a Feed Protocol in a Mouse Model That Mimics Marasmic Malnutrition
description	Host nutritional status directly interferes with immunity and/or susceptibility to infectious diseases. To understand the mechanisms behind this relationship, the use of animal models and feeding protocols is necessary. In the literature, studies reporting marasmic malnutrition in mice are not common. In this context, the objective of this study was to validate a feed methodology that mimics marasmic malnutrition, examining the nutritional, biochemical, and hematological status in BALB/c mice. Weaned BALB/c mice were or were not fed a Restricted diet (36.26% carbohydrate, 8.79% protein, 4.95% fat, and 7.62 kJ/100 g). Some malnourished mice underwent a refed process with a Control diet (65.93% carbohydrate, 24.18% protein, 9.89% fat, and 15.24 kJ/100 g). The nutritional status of the mice was evaluated through phenotypic markers and hematological and biochemical parameters. Our results showed that the Restricted diet was able to induce mild malnutrition in mice, resulting in mouse weight loss of 12%, which could be reversed after refeeding. Malnourished mice demonstrated slow body growth and low body mass index (BMI) values. Malnourished mice also showed physical and behavioral changes, a reduction of 47.5% in leukocyte counts and a 2-fold increase in cholesterol levels. In conclusion, our feeding protocol was able to generate mild malnutrition and cause changes in the nutritional status of mice that could be similar to those observed in marasmic malnutrition.
format	article
author	Taiana Ferreira-Paes Paula Seixas-Costa Elmo Eduardo Almeida-Amaral
author_facet	Taiana Ferreira-Paes Paula Seixas-Costa Elmo Eduardo Almeida-Amaral
author_sort	Taiana Ferreira-Paes
title	Validation of a Feed Protocol in a Mouse Model That Mimics Marasmic Malnutrition
title_short	Validation of a Feed Protocol in a Mouse Model That Mimics Marasmic Malnutrition
title_full	Validation of a Feed Protocol in a Mouse Model That Mimics Marasmic Malnutrition
title_fullStr	Validation of a Feed Protocol in a Mouse Model That Mimics Marasmic Malnutrition
title_full_unstemmed	Validation of a Feed Protocol in a Mouse Model That Mimics Marasmic Malnutrition
title_sort	validation of a feed protocol in a mouse model that mimics marasmic malnutrition
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ae881053145e4671adcdce9ffc4ef3fd
work_keys_str_mv	AT taianaferreirapaes validationofafeedprotocolinamousemodelthatmimicsmarasmicmalnutrition AT paulaseixascosta validationofafeedprotocolinamousemodelthatmimicsmarasmicmalnutrition AT elmoeduardoalmeidaamaral validationofafeedprotocolinamousemodelthatmimicsmarasmicmalnutrition
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Validation of a Feed Protocol in a Mouse Model That Mimics Marasmic Malnutrition

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