Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b

Backgrounds Acute myocardial infarction (MI) is the common clinical manifestation of coronary heart disease. Circular RNAs (circRNAs) act key roles in cardiomyocytes growth and angiogenesis. However, their functions in MI are not entirely clear. This research intended to investigate the role and und...

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Autores principales:	Yuquan Zhang, Shuzhu Liu, Limin Ding, Dawei Wang, Qiangqiang Li, Dongdong Li
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Myocardial infarction Circ_0030235 miR-526b PI3K/AKT and MEK/ERK pathways Medicine R
Acceso en línea:	https://doaj.org/article/ae88b68909f946c096797f1ba3c28465
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spelling	oai:doaj.org-article:ae88b68909f946c096797f1ba3c284652021-11-18T15:05:06ZCirc_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b10.7717/peerj.114822167-8359https://doaj.org/article/ae88b68909f946c096797f1ba3c284652021-11-01T00:00:00Zhttps://peerj.com/articles/11482.pdfhttps://peerj.com/articles/11482/https://doaj.org/toc/2167-8359Backgrounds Acute myocardial infarction (MI) is the common clinical manifestation of coronary heart disease. Circular RNAs (circRNAs) act key roles in cardiomyocytes growth and angiogenesis. However, their functions in MI are not entirely clear. This research intended to investigate the role and underlying mechanisms of circ_0030235 in H9c2 cells. Methods H9c2 cells were conducted to oxygen glucose deprivation/reperfusion (OGD/R) inducement to establish the MI model. Circ_0030235 and miR-526b expression was tested and altered by qRT-PCR and transfection. Cell viability, apoptosis and reactive oxygen species (ROS) injury were tested by CCK-8 assay, TUNEL assay kit, and ROS Detection Assay Kit, respectively. Assessment of cell injury-related factors was performed by employing ELISA, Mitochondrial Viability Staining and the JC-1-Mitochondrial Membrane Potential Assay Kit. The relationship between circ_0030235 and miR-526b was analyzed by dual luciferase reporter assay. The expression of key proteins was analyzed by western blot. Results Circ_0030235 was highly expressed in OGD/R-induced H9c2 cells. OGD/R inducement cell viability, while accelerated apoptosis. Besides, the level ROS, cell injury-related factors, mitochondrial membrane potential were notably elevated by OGD/R inducement, while mitochondrial viability was remarkably declined. Whereas, these impacts were all noticeably remitted by circ_0030235 knockdown. miR-526b was a target of circ_0030235. Circ_0030235 knockdown-induced impacts were all notably abrogated by miR-526b inhibition, including the activating impacts on PI3K/AKT and MEK/ERK pathways. Conclusions This research implied that circ_0030235 knockdown might remit OGD/R-induced impacts via activation of PI3K/AKT and MEK/ERK pathways and regulation of miR-526b.Yuquan ZhangShuzhu LiuLimin DingDawei WangQiangqiang LiDongdong LiPeerJ Inc.articleMyocardial infarctionCirc_0030235miR-526bPI3K/AKT and MEK/ERK pathwaysMedicineRENPeerJ, Vol 9, p e11482 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Myocardial infarction Circ_0030235 miR-526b PI3K/AKT and MEK/ERK pathways Medicine R
spellingShingle	Myocardial infarction Circ_0030235 miR-526b PI3K/AKT and MEK/ERK pathways Medicine R Yuquan Zhang Shuzhu Liu Limin Ding Dawei Wang Qiangqiang Li Dongdong Li Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b
description	Backgrounds Acute myocardial infarction (MI) is the common clinical manifestation of coronary heart disease. Circular RNAs (circRNAs) act key roles in cardiomyocytes growth and angiogenesis. However, their functions in MI are not entirely clear. This research intended to investigate the role and underlying mechanisms of circ_0030235 in H9c2 cells. Methods H9c2 cells were conducted to oxygen glucose deprivation/reperfusion (OGD/R) inducement to establish the MI model. Circ_0030235 and miR-526b expression was tested and altered by qRT-PCR and transfection. Cell viability, apoptosis and reactive oxygen species (ROS) injury were tested by CCK-8 assay, TUNEL assay kit, and ROS Detection Assay Kit, respectively. Assessment of cell injury-related factors was performed by employing ELISA, Mitochondrial Viability Staining and the JC-1-Mitochondrial Membrane Potential Assay Kit. The relationship between circ_0030235 and miR-526b was analyzed by dual luciferase reporter assay. The expression of key proteins was analyzed by western blot. Results Circ_0030235 was highly expressed in OGD/R-induced H9c2 cells. OGD/R inducement cell viability, while accelerated apoptosis. Besides, the level ROS, cell injury-related factors, mitochondrial membrane potential were notably elevated by OGD/R inducement, while mitochondrial viability was remarkably declined. Whereas, these impacts were all noticeably remitted by circ_0030235 knockdown. miR-526b was a target of circ_0030235. Circ_0030235 knockdown-induced impacts were all notably abrogated by miR-526b inhibition, including the activating impacts on PI3K/AKT and MEK/ERK pathways. Conclusions This research implied that circ_0030235 knockdown might remit OGD/R-induced impacts via activation of PI3K/AKT and MEK/ERK pathways and regulation of miR-526b.
format	article
author	Yuquan Zhang Shuzhu Liu Limin Ding Dawei Wang Qiangqiang Li Dongdong Li
author_facet	Yuquan Zhang Shuzhu Liu Limin Ding Dawei Wang Qiangqiang Li Dongdong Li
author_sort	Yuquan Zhang
title	Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b
title_short	Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b
title_full	Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b
title_fullStr	Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b
title_full_unstemmed	Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b
title_sort	circ_0030235 knockdown protects h9c2 cells against ogd/r-induced injury via regulation of mir-526b
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/ae88b68909f946c096797f1ba3c28465
work_keys_str_mv	AT yuquanzhang circ0030235knockdownprotectsh9c2cellsagainstogdrinducedinjuryviaregulationofmir526b AT shuzhuliu circ0030235knockdownprotectsh9c2cellsagainstogdrinducedinjuryviaregulationofmir526b AT liminding circ0030235knockdownprotectsh9c2cellsagainstogdrinducedinjuryviaregulationofmir526b AT daweiwang circ0030235knockdownprotectsh9c2cellsagainstogdrinducedinjuryviaregulationofmir526b AT qiangqiangli circ0030235knockdownprotectsh9c2cellsagainstogdrinducedinjuryviaregulationofmir526b AT dongdongli circ0030235knockdownprotectsh9c2cellsagainstogdrinducedinjuryviaregulationofmir526b
_version_	1718420804125851648

Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b

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