Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

Yanhua Hou, Kaiyong Cai, Jinghua Li, Xiuyong Chen, Min Lai, Yan Hu, Zhong Luo, Xingwei Ding, Dawei Xu Key Laboratory of Biorheological Science and Technology and Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, People’s Republic of China Background: The pu...

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Autores principales:	Hou Y, Cai K, Li J, Chen X, Lai M, Hu Y, Luo Z, Ding X, Xu D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ae9d21824a6f40909f89f886db0bbeba
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spelling	oai:doaj.org-article:ae9d21824a6f40909f89f886db0bbeba2021-12-02T03:52:04ZEffects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells1176-91141178-2013https://doaj.org/article/ae9d21824a6f40909f89f886db0bbeba2013-09-01T00:00:00Zhttp://www.dovepress.com/effects-of-titanium-nanoparticles-on-adhesion-migration-proliferation--a14446https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yanhua Hou, Kaiyong Cai, Jinghua Li, Xiuyong Chen, Min Lai, Yan Hu, Zhong Luo, Xingwei Ding, Dawei Xu Key Laboratory of Biorheological Science and Technology and Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, People’s Republic of China Background: The purpose of this study was to investigate the influences of nanoscale wear particles derived from titanium/titanium alloy-based implants on integration of bone. Here we report the potential impact of titanium oxide (TiO2) nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells (MSC) from the cellular level to the molecular level in the Wistar rat. Methods: A series of TiO2 nanoparticles (14 nm, 108 nm, and 196 nm) were synthesized and characterized by scanning electron microscopy and transmission electron microscopy, respectively. Results: The TiO2 nanoparticles had negative effects on cell viability, proliferation, and the cell cycle of MSC in a dose-dependent and size-dependent manner. Confocal laser scanning microscopy was used to investigate the effects of particle internalization on adhesion, spreading, and morphology of MSC. The integrity of the cell membrane, cytoskeleton, and vinculin of MSC were negatively influenced by large TiO2 nanoparticles. Conclusion: The Transwell migration assay and a wound healing model suggested that TiO2 nanoparticles had a strong adverse impact on cell migration as particle size increased (P < 0.01). Furthermore, alkaline phosphatase, gene expression of osteocalcin (OC) and osteopontin (OPN), and mineralization measurements indicate that the size of the TiO2 nanoparticles negatively affected osteogenic differentiation of MSC. Keywords: mesenchymal stem cells, titanium dioxide, nanoparticles, cytotoxicity, adhesion, migrationHou YCai KLi JChen XLai MHu YLuo ZDing XXu DDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 3619-3630 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Hou Y Cai K Li J Chen X Lai M Hu Y Luo Z Ding X Xu D Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells
description	Yanhua Hou, Kaiyong Cai, Jinghua Li, Xiuyong Chen, Min Lai, Yan Hu, Zhong Luo, Xingwei Ding, Dawei Xu Key Laboratory of Biorheological Science and Technology and Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, People’s Republic of China Background: The purpose of this study was to investigate the influences of nanoscale wear particles derived from titanium/titanium alloy-based implants on integration of bone. Here we report the potential impact of titanium oxide (TiO2) nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells (MSC) from the cellular level to the molecular level in the Wistar rat. Methods: A series of TiO2 nanoparticles (14 nm, 108 nm, and 196 nm) were synthesized and characterized by scanning electron microscopy and transmission electron microscopy, respectively. Results: The TiO2 nanoparticles had negative effects on cell viability, proliferation, and the cell cycle of MSC in a dose-dependent and size-dependent manner. Confocal laser scanning microscopy was used to investigate the effects of particle internalization on adhesion, spreading, and morphology of MSC. The integrity of the cell membrane, cytoskeleton, and vinculin of MSC were negatively influenced by large TiO2 nanoparticles. Conclusion: The Transwell migration assay and a wound healing model suggested that TiO2 nanoparticles had a strong adverse impact on cell migration as particle size increased (P < 0.01). Furthermore, alkaline phosphatase, gene expression of osteocalcin (OC) and osteopontin (OPN), and mineralization measurements indicate that the size of the TiO2 nanoparticles negatively affected osteogenic differentiation of MSC. Keywords: mesenchymal stem cells, titanium dioxide, nanoparticles, cytotoxicity, adhesion, migration
format	article
author	Hou Y Cai K Li J Chen X Lai M Hu Y Luo Z Ding X Xu D
author_facet	Hou Y Cai K Li J Chen X Lai M Hu Y Luo Z Ding X Xu D
author_sort	Hou Y
title	Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells
title_short	Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells
title_full	Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells
title_fullStr	Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells
title_full_unstemmed	Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells
title_sort	effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/ae9d21824a6f40909f89f886db0bbeba
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_version_	1718401610877501440

Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells

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