Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy

DNA covers the genetic information in all living organisms. Numerous intrinsic and extrinsic factors may influence the local structure of the DNA molecule or compromise its integrity. Detailed understanding of structural modifications of DNA resulting from interactions with other molecules and surro...

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Autores principales:	Ewelina Lipiec, Kamila Sofińska, Sara Seweryn, Natalia Wilkosz, Marek Szymonski
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	high-resolution Atomic Force Microscopy (AFM) DNA DNA structure molecular spectroscopy Tip-Enhanced Raman Spectroscopy (TERS) Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/ae9ea0f333ea4ed78e73ca84945eea17
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spelling	oai:doaj.org-article:ae9ea0f333ea4ed78e73ca84945eea172021-11-11T18:29:21ZRevealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy10.3390/molecules262164761420-3049https://doaj.org/article/ae9ea0f333ea4ed78e73ca84945eea172021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6476https://doaj.org/toc/1420-3049DNA covers the genetic information in all living organisms. Numerous intrinsic and extrinsic factors may influence the local structure of the DNA molecule or compromise its integrity. Detailed understanding of structural modifications of DNA resulting from interactions with other molecules and surrounding environment is of central importance for the future development of medicine and pharmacology. In this paper, we review the recent achievements in research on DNA structure at nanoscale. In particular, we focused on the molecular structure of DNA revealed by high-resolution AFM (Atomic Force Microscopy) imaging at liquid/solid interfaces. Such detailed structural studies were driven by the technical developments made in SPM (Scanning Probe Microscopy) techniques. Therefore, we describe here the working principles of AFM modes allowing high-resolution visualization of DNA structure under native (liquid) environment. While AFM provides well-resolved structure of molecules at nanoscale, it does not reveal the chemical structure and composition of studied samples. The simultaneous information combining the structural and chemical details of studied analyte allows achieve a comprehensive picture of investigated phenomenon. Therefore, we also summarize recent molecular spectroscopy studies, including Tip-Enhanced Raman Spectroscopy (TERS), on the DNA structure and its structural rearrangements.Ewelina LipiecKamila SofińskaSara SewerynNatalia WilkoszMarek SzymonskiMDPI AGarticlehigh-resolutionAtomic Force Microscopy (AFM)DNADNA structuremolecular spectroscopyTip-Enhanced Raman Spectroscopy (TERS)Organic chemistryQD241-441ENMolecules, Vol 26, Iss 6476, p 6476 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	high-resolution Atomic Force Microscopy (AFM) DNA DNA structure molecular spectroscopy Tip-Enhanced Raman Spectroscopy (TERS) Organic chemistry QD241-441
spellingShingle	high-resolution Atomic Force Microscopy (AFM) DNA DNA structure molecular spectroscopy Tip-Enhanced Raman Spectroscopy (TERS) Organic chemistry QD241-441 Ewelina Lipiec Kamila Sofińska Sara Seweryn Natalia Wilkosz Marek Szymonski Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy
description	DNA covers the genetic information in all living organisms. Numerous intrinsic and extrinsic factors may influence the local structure of the DNA molecule or compromise its integrity. Detailed understanding of structural modifications of DNA resulting from interactions with other molecules and surrounding environment is of central importance for the future development of medicine and pharmacology. In this paper, we review the recent achievements in research on DNA structure at nanoscale. In particular, we focused on the molecular structure of DNA revealed by high-resolution AFM (Atomic Force Microscopy) imaging at liquid/solid interfaces. Such detailed structural studies were driven by the technical developments made in SPM (Scanning Probe Microscopy) techniques. Therefore, we describe here the working principles of AFM modes allowing high-resolution visualization of DNA structure under native (liquid) environment. While AFM provides well-resolved structure of molecules at nanoscale, it does not reveal the chemical structure and composition of studied samples. The simultaneous information combining the structural and chemical details of studied analyte allows achieve a comprehensive picture of investigated phenomenon. Therefore, we also summarize recent molecular spectroscopy studies, including Tip-Enhanced Raman Spectroscopy (TERS), on the DNA structure and its structural rearrangements.
format	article
author	Ewelina Lipiec Kamila Sofińska Sara Seweryn Natalia Wilkosz Marek Szymonski
author_facet	Ewelina Lipiec Kamila Sofińska Sara Seweryn Natalia Wilkosz Marek Szymonski
author_sort	Ewelina Lipiec
title	Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy
title_short	Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy
title_full	Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy
title_fullStr	Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy
title_full_unstemmed	Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy
title_sort	revealing dna structure at liquid/solid interfaces by afm-based high-resolution imaging and molecular spectroscopy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ae9ea0f333ea4ed78e73ca84945eea17
work_keys_str_mv	AT ewelinalipiec revealingdnastructureatliquidsolidinterfacesbyafmbasedhighresolutionimagingandmolecularspectroscopy AT kamilasofinska revealingdnastructureatliquidsolidinterfacesbyafmbasedhighresolutionimagingandmolecularspectroscopy AT saraseweryn revealingdnastructureatliquidsolidinterfacesbyafmbasedhighresolutionimagingandmolecularspectroscopy AT nataliawilkosz revealingdnastructureatliquidsolidinterfacesbyafmbasedhighresolutionimagingandmolecularspectroscopy AT marekszymonski revealingdnastructureatliquidsolidinterfacesbyafmbasedhighresolutionimagingandmolecularspectroscopy
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Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy

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