Proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality

Abstract The regulation of wheat protein quality is a highly complex biological process involving multiple metabolic pathways. To reveal new insights into the regulatory pathways of wheat glutenin synthesis, we used the grain-filling period wheat grains of the near-isogenic lines NIL-723 and NIL-101...

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Autores principales:	Liangjie Lv, Aiju Zhao, Yelun Zhang, Hui Li, Xiyong Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aeaa82f48ba84e4086eb6137ad48832f
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spelling	oai:doaj.org-article:aeaa82f48ba84e4086eb6137ad48832f2021-12-02T17:02:13ZProteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality10.1038/s41598-021-89140-42045-2322https://doaj.org/article/aeaa82f48ba84e4086eb6137ad48832f2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89140-4https://doaj.org/toc/2045-2322Abstract The regulation of wheat protein quality is a highly complex biological process involving multiple metabolic pathways. To reveal new insights into the regulatory pathways of wheat glutenin synthesis, we used the grain-filling period wheat grains of the near-isogenic lines NIL-723 and NIL-1010, which have large differences in quality, to perform a combined transcriptome and proteome analysis. Compared with NIL-1010, NIL-723 had 1287 transcripts and 355 proteins with significantly different abundances. Certain key significantly enriched pathway were identified, and wheat quality was associated with alanine, aspartate and glutamate metabolism, nitrogen metabolism and alpha-linolenic acid metabolism. Differentially expressed proteins (DEPs) or Differentially expressed genes (DEGs) in amino acid synthesis pathways were upregulated primarily in the glycine (Gly), methionine (Met), threonine (Thr), glutamic acid (Glu), proline (proC), cysteine (Cys), and arginine (Arg) synthesis and downregulated in the tryptophan (trpE), leucine (leuC), citrulline (argE), and ornithine (argE) synthesis. Furthermore, to elucidate changes in glutenin in the grain synthesis pathway, we plotted a regulatory pathway map and found that DEGs and DEPs in ribosomes (RPL5) and the ER (HSPA5, HYOU1, PDIA3, PDIA1, Sec24, and Sec31) may play key roles in regulating glutenin synthesis. The transcriptional validation of some of the differentially expressed proteins through real-time quantitative PCR analysis further validated the transcriptome and proteomic results.Liangjie LvAiju ZhaoYelun ZhangHui LiXiyong ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Liangjie Lv Aiju Zhao Yelun Zhang Hui Li Xiyong Chen Proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality
description	Abstract The regulation of wheat protein quality is a highly complex biological process involving multiple metabolic pathways. To reveal new insights into the regulatory pathways of wheat glutenin synthesis, we used the grain-filling period wheat grains of the near-isogenic lines NIL-723 and NIL-1010, which have large differences in quality, to perform a combined transcriptome and proteome analysis. Compared with NIL-1010, NIL-723 had 1287 transcripts and 355 proteins with significantly different abundances. Certain key significantly enriched pathway were identified, and wheat quality was associated with alanine, aspartate and glutamate metabolism, nitrogen metabolism and alpha-linolenic acid metabolism. Differentially expressed proteins (DEPs) or Differentially expressed genes (DEGs) in amino acid synthesis pathways were upregulated primarily in the glycine (Gly), methionine (Met), threonine (Thr), glutamic acid (Glu), proline (proC), cysteine (Cys), and arginine (Arg) synthesis and downregulated in the tryptophan (trpE), leucine (leuC), citrulline (argE), and ornithine (argE) synthesis. Furthermore, to elucidate changes in glutenin in the grain synthesis pathway, we plotted a regulatory pathway map and found that DEGs and DEPs in ribosomes (RPL5) and the ER (HSPA5, HYOU1, PDIA3, PDIA1, Sec24, and Sec31) may play key roles in regulating glutenin synthesis. The transcriptional validation of some of the differentially expressed proteins through real-time quantitative PCR analysis further validated the transcriptome and proteomic results.
format	article
author	Liangjie Lv Aiju Zhao Yelun Zhang Hui Li Xiyong Chen
author_facet	Liangjie Lv Aiju Zhao Yelun Zhang Hui Li Xiyong Chen
author_sort	Liangjie Lv
title	Proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality
title_short	Proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality
title_full	Proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality
title_fullStr	Proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality
title_full_unstemmed	Proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality
title_sort	proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aeaa82f48ba84e4086eb6137ad48832f
work_keys_str_mv	AT liangjielv proteomeandtranscriptomeanalysesofwheatnearisogeniclinesidentifieskeyproteinsandgenesofwheatbreadquality AT aijuzhao proteomeandtranscriptomeanalysesofwheatnearisogeniclinesidentifieskeyproteinsandgenesofwheatbreadquality AT yelunzhang proteomeandtranscriptomeanalysesofwheatnearisogeniclinesidentifieskeyproteinsandgenesofwheatbreadquality AT huili proteomeandtranscriptomeanalysesofwheatnearisogeniclinesidentifieskeyproteinsandgenesofwheatbreadquality AT xiyongchen proteomeandtranscriptomeanalysesofwheatnearisogeniclinesidentifieskeyproteinsandgenesofwheatbreadquality
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Proteome and transcriptome analyses of wheat near isogenic lines identifies key proteins and genes of wheat bread quality

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