Gene transfection in primary stem-like cells of giant cell tumor of bone

Shalini Singh1, Isabella Mak1, Patricia Power1, Melissa Cunnigham2, Robert Turcotte3, Michelle Ghert11Departments of Surgery, 2Biology, McMaster University, Hamilton, Ontario; 3Department of Orthopaedic Surgery, McGill University Medical Centre, Montreal, Quebec, CanadaAbstract: The neoplastic stem-...

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Autores principales:	Shalini Singh, Isabella Mak, Patricia Power, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/aeac0f95b7c54ce9ac7906e088c6fece
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spelling	oai:doaj.org-article:aeac0f95b7c54ce9ac7906e088c6fece2021-12-02T05:10:17ZGene transfection in primary stem-like cells of giant cell tumor of bone1178-6957https://doaj.org/article/aeac0f95b7c54ce9ac7906e088c6fece2010-09-01T00:00:00Zhttp://www.dovepress.com/gene-transfection-in-primary-stem-like-cells-of-giant-cell-tumor-of-bo-a5387https://doaj.org/toc/1178-6957Shalini Singh1, Isabella Mak1, Patricia Power1, Melissa Cunnigham2, Robert Turcotte3, Michelle Ghert11Departments of Surgery, 2Biology, McMaster University, Hamilton, Ontario; 3Department of Orthopaedic Surgery, McGill University Medical Centre, Montreal, Quebec, CanadaAbstract: The neoplastic stem-like stromal cell of giant cell tumor of bone (GCT) survives for multiple passages in primary culture with a stable phenotype, and exhibits multipotent characteristics. The pathophysiology of this tumor has been studied through the primary culture of these cells. However, successful gene transfer of these cells has not been reported to date. In this short report, we describe the development of the first reported technique that results in efficient gene transfection in primary stem-like cells of GCT.Keywords: gene, transfection, primary cells, TWIST, giant cell tumor Shalini SinghIsabella MakPatricia Poweret alDove Medical PressarticleCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol 2010, Iss default, Pp 129-134 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Shalini Singh Isabella Mak Patricia Power et al Gene transfection in primary stem-like cells of giant cell tumor of bone
description	Shalini Singh1, Isabella Mak1, Patricia Power1, Melissa Cunnigham2, Robert Turcotte3, Michelle Ghert11Departments of Surgery, 2Biology, McMaster University, Hamilton, Ontario; 3Department of Orthopaedic Surgery, McGill University Medical Centre, Montreal, Quebec, CanadaAbstract: The neoplastic stem-like stromal cell of giant cell tumor of bone (GCT) survives for multiple passages in primary culture with a stable phenotype, and exhibits multipotent characteristics. The pathophysiology of this tumor has been studied through the primary culture of these cells. However, successful gene transfer of these cells has not been reported to date. In this short report, we describe the development of the first reported technique that results in efficient gene transfection in primary stem-like cells of GCT.Keywords: gene, transfection, primary cells, TWIST, giant cell tumor
format	article
author	Shalini Singh Isabella Mak Patricia Power et al
author_facet	Shalini Singh Isabella Mak Patricia Power et al
author_sort	Shalini Singh
title	Gene transfection in primary stem-like cells of giant cell tumor of bone
title_short	Gene transfection in primary stem-like cells of giant cell tumor of bone
title_full	Gene transfection in primary stem-like cells of giant cell tumor of bone
title_fullStr	Gene transfection in primary stem-like cells of giant cell tumor of bone
title_full_unstemmed	Gene transfection in primary stem-like cells of giant cell tumor of bone
title_sort	gene transfection in primary stem-like cells of giant cell tumor of bone
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/aeac0f95b7c54ce9ac7906e088c6fece
work_keys_str_mv	AT shalinisingh genetransfectioninprimarystemlikecellsofgiantcelltumorofbone AT isabellamak genetransfectioninprimarystemlikecellsofgiantcelltumorofbone AT patriciapower genetransfectioninprimarystemlikecellsofgiantcelltumorofbone AT etal genetransfectioninprimarystemlikecellsofgiantcelltumorofbone
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Gene transfection in primary stem-like cells of giant cell tumor of bone

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