A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.

Beyond their canonical function in nucleocytoplasmic exchanges, nuclear pore complexes (NPCs) regulate the expression of protein-coding genes. Here, we have implemented transcriptomic and molecular methods to specifically address the impact of the NPC on retroelements, which are present in multiple...

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Autores principales:	Amandine Bonnet, Carole Chaput, Noé Palmic, Benoit Palancade, Pascale Lesage
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/aeb92a8672c041b4a5c06e88792127ba
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spelling	oai:doaj.org-article:aeb92a8672c041b4a5c06e88792127ba2021-12-02T20:03:17ZA nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.1553-73901553-740410.1371/journal.pgen.1009889https://doaj.org/article/aeb92a8672c041b4a5c06e88792127ba2021-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009889https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Beyond their canonical function in nucleocytoplasmic exchanges, nuclear pore complexes (NPCs) regulate the expression of protein-coding genes. Here, we have implemented transcriptomic and molecular methods to specifically address the impact of the NPC on retroelements, which are present in multiple copies in genomes. We report a novel function for the Nup84 complex, a core NPC building block, in specifically restricting the transcription of LTR-retrotransposons in yeast. Nup84 complex-dependent repression impacts both Copia and Gypsy Ty LTR-retrotransposons, all over the S. cerevisiae genome. Mechanistically, the Nup84 complex restricts the transcription of Ty1, the most active yeast retrotransposon, through the tethering of the SUMO-deconjugating enzyme Ulp1 to NPCs. Strikingly, the modest accumulation of Ty1 RNAs caused by Nup84 complex loss-of-function is sufficient to trigger an important increase of Ty1 cDNA levels, resulting in massive Ty1 retrotransposition. Altogether, our study expands our understanding of the complex interactions between retrotransposons and the NPC, and highlights the importance for the cells to keep retrotransposons under tight transcriptional control.Amandine BonnetCarole ChaputNoé PalmicBenoit PalancadePascale LesagePublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 11, p e1009889 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Amandine Bonnet Carole Chaput Noé Palmic Benoit Palancade Pascale Lesage A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.
description	Beyond their canonical function in nucleocytoplasmic exchanges, nuclear pore complexes (NPCs) regulate the expression of protein-coding genes. Here, we have implemented transcriptomic and molecular methods to specifically address the impact of the NPC on retroelements, which are present in multiple copies in genomes. We report a novel function for the Nup84 complex, a core NPC building block, in specifically restricting the transcription of LTR-retrotransposons in yeast. Nup84 complex-dependent repression impacts both Copia and Gypsy Ty LTR-retrotransposons, all over the S. cerevisiae genome. Mechanistically, the Nup84 complex restricts the transcription of Ty1, the most active yeast retrotransposon, through the tethering of the SUMO-deconjugating enzyme Ulp1 to NPCs. Strikingly, the modest accumulation of Ty1 RNAs caused by Nup84 complex loss-of-function is sufficient to trigger an important increase of Ty1 cDNA levels, resulting in massive Ty1 retrotransposition. Altogether, our study expands our understanding of the complex interactions between retrotransposons and the NPC, and highlights the importance for the cells to keep retrotransposons under tight transcriptional control.
format	article
author	Amandine Bonnet Carole Chaput Noé Palmic Benoit Palancade Pascale Lesage
author_facet	Amandine Bonnet Carole Chaput Noé Palmic Benoit Palancade Pascale Lesage
author_sort	Amandine Bonnet
title	A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.
title_short	A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.
title_full	A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.
title_fullStr	A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.
title_full_unstemmed	A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.
title_sort	nuclear pore sub-complex restricts the propagation of ty retrotransposons by limiting their transcription.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/aeb92a8672c041b4a5c06e88792127ba
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A nuclear pore sub-complex restricts the propagation of Ty retrotransposons by limiting their transcription.

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