Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering

Abstract The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues. In this study, polypyrrole (PPy)–alginate (Alg) composites were prepared using different percentages of alginate a...

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Autores principales:	Asma Manzari-Tavakoli, Roghayeh Tarasi, Roya Sedghi, Ali Moghimi, Hassan Niknejad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aeb9990f16ec478491cc1e11dec1ae28
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spelling	oai:doaj.org-article:aeb9990f16ec478491cc1e11dec1ae282021-12-02T12:42:18ZFabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering10.1038/s41598-020-78650-22045-2322https://doaj.org/article/aeb9990f16ec478491cc1e11dec1ae282020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78650-2https://doaj.org/toc/2045-2322Abstract The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues. In this study, polypyrrole (PPy)–alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method. In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy–Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold. Physiochemical characterization of nanochitosan/PPy–Alg scaffold such as electrical conductivity, porosity, swelling and degradation was investigated. Moreover, cytotoxicity and proliferation were examined by culturing OLN-93 neural and human dermal fibroblasts cells on the Nanochitosan/PPy–Alg scaffold. Due to the high conductivity, the film with ratio 2:10 (PPy–Alg) was recognized more suitable for fabrication of the final scaffold. Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy–Alg scaffold. Based on the features of the constructed scaffold, Nanochitosan/PPy–Alg scaffold can be a proper candidate for neural tissue engineering.Asma Manzari-TavakoliRoghayeh TarasiRoya SedghiAli MoghimiHassan NiknejadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Asma Manzari-Tavakoli Roghayeh Tarasi Roya Sedghi Ali Moghimi Hassan Niknejad Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
description	Abstract The utilization of conductive polymers for fabrication of neural scaffolds have attracted much interest because of providing a microenvironment which can imitate nerve tissues. In this study, polypyrrole (PPy)–alginate (Alg) composites were prepared using different percentages of alginate and pyrrole by oxidative polymerization method using FeCl3 as an oxidant and electrical conductivity of composites were measured by four probe method. In addition, chitosan-based nanoparticles were synthesized by ionic gelation method and after characterization merged into PPy–Alg composite in order to fabricate a conductive, hydrophilic, processable and stable scaffold. Physiochemical characterization of nanochitosan/PPy–Alg scaffold such as electrical conductivity, porosity, swelling and degradation was investigated. Moreover, cytotoxicity and proliferation were examined by culturing OLN-93 neural and human dermal fibroblasts cells on the Nanochitosan/PPy–Alg scaffold. Due to the high conductivity, the film with ratio 2:10 (PPy–Alg) was recognized more suitable for fabrication of the final scaffold. Results from FT-IR and SEM, evaluation of porosity, swelling and degradation, as well as viability and proliferation of OLN-93 neural and fibroblast cells confirmed cytocompatiblity of the Nanochitosan/PPy–Alg scaffold. Based on the features of the constructed scaffold, Nanochitosan/PPy–Alg scaffold can be a proper candidate for neural tissue engineering.
format	article
author	Asma Manzari-Tavakoli Roghayeh Tarasi Roya Sedghi Ali Moghimi Hassan Niknejad
author_facet	Asma Manzari-Tavakoli Roghayeh Tarasi Roya Sedghi Ali Moghimi Hassan Niknejad
author_sort	Asma Manzari-Tavakoli
title	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_short	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_full	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_fullStr	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_full_unstemmed	Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
title_sort	fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/aeb9990f16ec478491cc1e11dec1ae28
work_keys_str_mv	AT asmamanzaritavakoli fabricationofnanochitosanincorporatedpolypyrrolealginateconductingscaffoldforneuraltissueengineering AT roghayehtarasi fabricationofnanochitosanincorporatedpolypyrrolealginateconductingscaffoldforneuraltissueengineering AT royasedghi fabricationofnanochitosanincorporatedpolypyrrolealginateconductingscaffoldforneuraltissueengineering AT alimoghimi fabricationofnanochitosanincorporatedpolypyrrolealginateconductingscaffoldforneuraltissueengineering AT hassanniknejad fabricationofnanochitosanincorporatedpolypyrrolealginateconductingscaffoldforneuraltissueengineering
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Fabrication of nanochitosan incorporated polypyrrole/alginate conducting scaffold for neural tissue engineering

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