Enhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration

Abstract Interstitial fluid (ISF) is a body fluid that fills, surrounds cells and contains various biomarkers, but it has been challenging to extract ISF in a reliable and sufficient amount with high speed. To address the issues, we developed the tilted microneedle ISF collecting system (TMICS) fabr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sanha Kim, Min Suk Lee, Hee Seok Yang, Jae Hwan Jung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aec1daa6d2bd4398a75259e5a81748b8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:aec1daa6d2bd4398a75259e5a81748b8
record_format	dspace
spelling	oai:doaj.org-article:aec1daa6d2bd4398a75259e5a81748b82021-12-02T16:14:47ZEnhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration10.1038/s41598-021-93235-32045-2322https://doaj.org/article/aec1daa6d2bd4398a75259e5a81748b82021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93235-3https://doaj.org/toc/2045-2322Abstract Interstitial fluid (ISF) is a body fluid that fills, surrounds cells and contains various biomarkers, but it has been challenging to extract ISF in a reliable and sufficient amount with high speed. To address the issues, we developed the tilted microneedle ISF collecting system (TMICS) fabricated by 3D printing. In this system, the microneedle (MN) was inserted at 66° to the skin by TMICS so that the MN length could be extended within a safe range of skin penetration. Moreover, TMICS incorporating three MN patches created reliable ISF collecting conditions by penetrating the skin at consistent angle and force, 4.9 N. Due to the MN length increase and the patch number expansion, the surface area of the penetrated tissue was increased, thereby confirming that ISF extraction efficiency was improved. Skin ISF was collected into the paper reservoir on the patch, and the absorbed area was converted into a volume. ISF extraction from the rat skin in vivo by TMICS was well tolerated, and the 2.9 μL of ISF was obtained within 30 s. Therefore, TMICS is promising to apply in the diagnosis of multiple biomarkers in ISF with high speed and stability.Sanha KimMin Suk LeeHee Seok YangJae Hwan JungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sanha Kim Min Suk Lee Hee Seok Yang Jae Hwan Jung Enhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration
description	Abstract Interstitial fluid (ISF) is a body fluid that fills, surrounds cells and contains various biomarkers, but it has been challenging to extract ISF in a reliable and sufficient amount with high speed. To address the issues, we developed the tilted microneedle ISF collecting system (TMICS) fabricated by 3D printing. In this system, the microneedle (MN) was inserted at 66° to the skin by TMICS so that the MN length could be extended within a safe range of skin penetration. Moreover, TMICS incorporating three MN patches created reliable ISF collecting conditions by penetrating the skin at consistent angle and force, 4.9 N. Due to the MN length increase and the patch number expansion, the surface area of the penetrated tissue was increased, thereby confirming that ISF extraction efficiency was improved. Skin ISF was collected into the paper reservoir on the patch, and the absorbed area was converted into a volume. ISF extraction from the rat skin in vivo by TMICS was well tolerated, and the 2.9 μL of ISF was obtained within 30 s. Therefore, TMICS is promising to apply in the diagnosis of multiple biomarkers in ISF with high speed and stability.
format	article
author	Sanha Kim Min Suk Lee Hee Seok Yang Jae Hwan Jung
author_facet	Sanha Kim Min Suk Lee Hee Seok Yang Jae Hwan Jung
author_sort	Sanha Kim
title	Enhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration
title_short	Enhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration
title_full	Enhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration
title_fullStr	Enhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration
title_full_unstemmed	Enhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration
title_sort	enhanced extraction of skin interstitial fluid using a 3d printed device enabling tilted microneedle penetration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aec1daa6d2bd4398a75259e5a81748b8
work_keys_str_mv	AT sanhakim enhancedextractionofskininterstitialfluidusinga3dprinteddeviceenablingtiltedmicroneedlepenetration AT minsuklee enhancedextractionofskininterstitialfluidusinga3dprinteddeviceenablingtiltedmicroneedlepenetration AT heeseokyang enhancedextractionofskininterstitialfluidusinga3dprinteddeviceenablingtiltedmicroneedlepenetration AT jaehwanjung enhancedextractionofskininterstitialfluidusinga3dprinteddeviceenablingtiltedmicroneedlepenetration
_version_	1718384312262328320

Enhanced extraction of skin interstitial fluid using a 3D printed device enabling tilted microneedle penetration

Ejemplares similares