Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis

Steven D Newby,1,2 Tom Masi,3 Christopher D Griffin,4 William J King,4 Anna Chipman,1 Stacy Stephenson,3 David E Anderson,1 Alexandru S Biris,4 Shawn E Bourdo,4 Madhu Dhar1 1College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA; 2Comparative and Experimental Medicine, Uni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Newby SD, Masi T, Griffin CD, King WJ, Chipman A, Stephenson S, Anderson DE, Biris AS, Bourdo SE, Dhar M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	graphene nanoparticles functionalized graphene human mesenchymal stem cells extracellular matrix fibronectin collagen i osteogenic niche Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/aec55ccdcc964c9caac36b5faa07adbf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:aec55ccdcc964c9caac36b5faa07adbf
record_format	dspace
spelling	oai:doaj.org-article:aec55ccdcc964c9caac36b5faa07adbf2021-12-02T09:56:20ZFunctionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis1178-2013https://doaj.org/article/aec55ccdcc964c9caac36b5faa07adbf2020-04-01T00:00:00Zhttps://www.dovepress.com/functionalized-graphene-nanoparticles-induce-human-mesenchymal-stem-ce-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Steven D Newby,1,2 Tom Masi,3 Christopher D Griffin,4 William J King,4 Anna Chipman,1 Stacy Stephenson,3 David E Anderson,1 Alexandru S Biris,4 Shawn E Bourdo,4 Madhu Dhar1 1College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA; 2Comparative and Experimental Medicine, University of Tennessee, Knoxville, TN 37996, USA; 3University of Tennessee Graduate School of Medicine, Knoxville, TN 37996, USA; 4Center for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USACorrespondence: Madhu DharCollege of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USATel +1865-974-5703Fax + 1865-974-5773Email mdhar@utk.eduShawn E BourdoCenter for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USATel +1501-683-7222Fax +1501-683-7601Email sxbourdo@ualr.eduPurpose: The extracellular matrix (ECM) labyrinthine network secreted by mesenchymal stem cells (MSCs) provides a microenvironment that enhances cell adherence, proliferation, viability, and differentiation. The potential of graphene-based nanomaterials to mimic a tissue-specific ECM has been recognized in designing bone tissue engineering scaffolds. In this study, we investigated the expression of specific ECM proteins when human fat-derived adult MSCs adhered and underwent osteogenic differentiation in the presence of functionalized graphene nanoparticles.Methods: Graphene nanoparticles with 6– 10% oxygen content were prepared and characterized by XPS, FTIR, AFM and Raman spectroscopy. Calcein-am and crystal violet staining were performed to evaluate viability and proliferation of human fat-derived MSCs on graphene nanoparticles. Alizarin red staining and quantitation were used to determine the effect of graphene nanoparticles on osteogenic differentiation. Finally, immunofluorescence assays were used to investigate the expression of ECM proteins during cell adhesion and osteogenic differentiation.Results: Our data show that in the presence of graphene, MSCs express specific integrin heterodimers and exhibit a distinct pattern of the corresponding bone-specific ECM proteins, primarily fibronectin, collagen I and vitronectin. Furthermore, MSCs undergo osteogenic differentiation spontaneously without any chemical induction, suggesting that the physicochemical properties of graphene nanoparticles might trigger the expression of bone-specific ECM.Conclusion: Understanding the cell–graphene interactions resulting in an osteogenic niche for MSCs will significantly improve the application of graphene nanoparticles in bone repair and regeneration.Keywords: graphene nanoparticles, functionalized graphene, human mesenchymal stem cells, extracellular matrix, fibronectin, collagen I, osteogenic niche  Newby SDMasi TGriffin CDKing WJChipman AStephenson SAnderson DEBiris ASBourdo SEDhar MDove Medical Pressarticlegraphene nanoparticlesfunctionalized graphenehuman mesenchymal stem cellsextracellular matrixfibronectincollagen iosteogenic nicheMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2501-2513 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	graphene nanoparticles functionalized graphene human mesenchymal stem cells extracellular matrix fibronectin collagen i osteogenic niche Medicine (General) R5-920
spellingShingle	graphene nanoparticles functionalized graphene human mesenchymal stem cells extracellular matrix fibronectin collagen i osteogenic niche Medicine (General) R5-920 Newby SD Masi T Griffin CD King WJ Chipman A Stephenson S Anderson DE Biris AS Bourdo SE Dhar M Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis
description	Steven D Newby,1,2 Tom Masi,3 Christopher D Griffin,4 William J King,4 Anna Chipman,1 Stacy Stephenson,3 David E Anderson,1 Alexandru S Biris,4 Shawn E Bourdo,4 Madhu Dhar1 1College of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USA; 2Comparative and Experimental Medicine, University of Tennessee, Knoxville, TN 37996, USA; 3University of Tennessee Graduate School of Medicine, Knoxville, TN 37996, USA; 4Center for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USACorrespondence: Madhu DharCollege of Veterinary Medicine, University of Tennessee, Knoxville, TN 37996, USATel +1865-974-5703Fax + 1865-974-5773Email mdhar@utk.eduShawn E BourdoCenter for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USATel +1501-683-7222Fax +1501-683-7601Email sxbourdo@ualr.eduPurpose: The extracellular matrix (ECM) labyrinthine network secreted by mesenchymal stem cells (MSCs) provides a microenvironment that enhances cell adherence, proliferation, viability, and differentiation. The potential of graphene-based nanomaterials to mimic a tissue-specific ECM has been recognized in designing bone tissue engineering scaffolds. In this study, we investigated the expression of specific ECM proteins when human fat-derived adult MSCs adhered and underwent osteogenic differentiation in the presence of functionalized graphene nanoparticles.Methods: Graphene nanoparticles with 6– 10% oxygen content were prepared and characterized by XPS, FTIR, AFM and Raman spectroscopy. Calcein-am and crystal violet staining were performed to evaluate viability and proliferation of human fat-derived MSCs on graphene nanoparticles. Alizarin red staining and quantitation were used to determine the effect of graphene nanoparticles on osteogenic differentiation. Finally, immunofluorescence assays were used to investigate the expression of ECM proteins during cell adhesion and osteogenic differentiation.Results: Our data show that in the presence of graphene, MSCs express specific integrin heterodimers and exhibit a distinct pattern of the corresponding bone-specific ECM proteins, primarily fibronectin, collagen I and vitronectin. Furthermore, MSCs undergo osteogenic differentiation spontaneously without any chemical induction, suggesting that the physicochemical properties of graphene nanoparticles might trigger the expression of bone-specific ECM.Conclusion: Understanding the cell–graphene interactions resulting in an osteogenic niche for MSCs will significantly improve the application of graphene nanoparticles in bone repair and regeneration.Keywords: graphene nanoparticles, functionalized graphene, human mesenchymal stem cells, extracellular matrix, fibronectin, collagen I, osteogenic niche
format	article
author	Newby SD Masi T Griffin CD King WJ Chipman A Stephenson S Anderson DE Biris AS Bourdo SE Dhar M
author_facet	Newby SD Masi T Griffin CD King WJ Chipman A Stephenson S Anderson DE Biris AS Bourdo SE Dhar M
author_sort	Newby SD
title	Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis
title_short	Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis
title_full	Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis
title_fullStr	Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis
title_full_unstemmed	Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis
title_sort	functionalized graphene nanoparticles induce human mesenchymal stem cells to express distinct extracellular matrix proteins mediating osteogenesis
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/aec55ccdcc964c9caac36b5faa07adbf
work_keys_str_mv	AT newbysd functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis AT masit functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis AT griffincd functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis AT kingwj functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis AT chipmana functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis AT stephensons functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis AT andersonde functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis AT birisas functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis AT bourdose functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis AT dharm functionalizedgraphenenanoparticlesinducehumanmesenchymalstemcellstoexpressdistinctextracellularmatrixproteinsmediatingosteogenesis
_version_	1718397897800679424

Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis

Ejemplares similares