Vaginal microbiome topic modeling of laboring Ugandan women with and without fever
Abstract The composition of the maternal vaginal microbiome influences the duration of pregnancy, onset of labor, and even neonatal outcomes. Maternal microbiome research in sub-Saharan Africa has focused on non-pregnant and postpartum composition of the vaginal microbiome. Here we aimed to illustra...
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Nature Portfolio
2021
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oai:doaj.org-article:aec5a945eb0b423e9ee84dede5fe278d2021-12-02T17:41:18ZVaginal microbiome topic modeling of laboring Ugandan women with and without fever10.1038/s41522-021-00244-12055-5008https://doaj.org/article/aec5a945eb0b423e9ee84dede5fe278d2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00244-1https://doaj.org/toc/2055-5008Abstract The composition of the maternal vaginal microbiome influences the duration of pregnancy, onset of labor, and even neonatal outcomes. Maternal microbiome research in sub-Saharan Africa has focused on non-pregnant and postpartum composition of the vaginal microbiome. Here we aimed to illustrate the relationship between the vaginal microbiome of 99 laboring Ugandan women and intrapartum fever using routine microbiology and 16S ribosomal RNA gene sequencing from two hypervariable regions (V1–V2 and V3–V4). To describe the vaginal microbes associated with vaginal microbial communities, we pursued two approaches: hierarchical clustering methods and a novel Grades of Membership (GoM) modeling approach for vaginal microbiome characterization. Leveraging GoM models, we created a basis composed of a preassigned number of microbial topics whose linear combination optimally represents each patient yielding more comprehensive associations and characterization between maternal clinical features and the microbial communities. Using a random forest model, we showed that by including microbial topic models we improved upon clinical variables to predict maternal fever. Overall, we found a higher prevalence of Granulicatella, Streptococcus, Fusobacterium, Anaerococcus, Sneathia, Clostridium, Gemella, Mobiluncus, and Veillonella genera in febrile mothers, and higher prevalence of Lactobacillus genera (in particular L. crispatus and L. jensenii), Acinobacter, Aerococcus, and Prevotella species in afebrile mothers. By including clinical variables with microbial topics in this model, we observed young maternal age, fever reported earlier in the pregnancy, longer labor duration, and microbial communities with reduced Lactobacillus diversity were associated with intrapartum fever. These results better defined relationships between the presence or absence of intrapartum fever, demographics, peripartum course, and vaginal microbial topics, and expanded our understanding of the impact of the microbiome on maternal and potentially neonatal outcome risk.Mercedeh MovassaghLisa M. BebellKathy BurgoineChristine HehnlyLijun ZhangKim MoranKathryn SheldonShamim A. SinnarEdith Mbabazi-KabachelorElias KumbakumbaJoel BaziraMoses OchoraRonnie MulondoBrian Kaaya NsubugaAndrew D. WeeksMelissa GladstonePeter Olupot-OlupotJoseph NgonziDrucilla J. RobertsFrederick A. MeierRafael A. IrizarryJames R. BroachSteven J. SchiffJoseph N. PaulsonNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-10 (2021) |
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| topic |
Microbial ecology QR100-130 |
| spellingShingle |
Microbial ecology QR100-130 Mercedeh Movassagh Lisa M. Bebell Kathy Burgoine Christine Hehnly Lijun Zhang Kim Moran Kathryn Sheldon Shamim A. Sinnar Edith Mbabazi-Kabachelor Elias Kumbakumba Joel Bazira Moses Ochora Ronnie Mulondo Brian Kaaya Nsubuga Andrew D. Weeks Melissa Gladstone Peter Olupot-Olupot Joseph Ngonzi Drucilla J. Roberts Frederick A. Meier Rafael A. Irizarry James R. Broach Steven J. Schiff Joseph N. Paulson Vaginal microbiome topic modeling of laboring Ugandan women with and without fever |
| description |
Abstract The composition of the maternal vaginal microbiome influences the duration of pregnancy, onset of labor, and even neonatal outcomes. Maternal microbiome research in sub-Saharan Africa has focused on non-pregnant and postpartum composition of the vaginal microbiome. Here we aimed to illustrate the relationship between the vaginal microbiome of 99 laboring Ugandan women and intrapartum fever using routine microbiology and 16S ribosomal RNA gene sequencing from two hypervariable regions (V1–V2 and V3–V4). To describe the vaginal microbes associated with vaginal microbial communities, we pursued two approaches: hierarchical clustering methods and a novel Grades of Membership (GoM) modeling approach for vaginal microbiome characterization. Leveraging GoM models, we created a basis composed of a preassigned number of microbial topics whose linear combination optimally represents each patient yielding more comprehensive associations and characterization between maternal clinical features and the microbial communities. Using a random forest model, we showed that by including microbial topic models we improved upon clinical variables to predict maternal fever. Overall, we found a higher prevalence of Granulicatella, Streptococcus, Fusobacterium, Anaerococcus, Sneathia, Clostridium, Gemella, Mobiluncus, and Veillonella genera in febrile mothers, and higher prevalence of Lactobacillus genera (in particular L. crispatus and L. jensenii), Acinobacter, Aerococcus, and Prevotella species in afebrile mothers. By including clinical variables with microbial topics in this model, we observed young maternal age, fever reported earlier in the pregnancy, longer labor duration, and microbial communities with reduced Lactobacillus diversity were associated with intrapartum fever. These results better defined relationships between the presence or absence of intrapartum fever, demographics, peripartum course, and vaginal microbial topics, and expanded our understanding of the impact of the microbiome on maternal and potentially neonatal outcome risk. |
| format |
article |
| author |
Mercedeh Movassagh Lisa M. Bebell Kathy Burgoine Christine Hehnly Lijun Zhang Kim Moran Kathryn Sheldon Shamim A. Sinnar Edith Mbabazi-Kabachelor Elias Kumbakumba Joel Bazira Moses Ochora Ronnie Mulondo Brian Kaaya Nsubuga Andrew D. Weeks Melissa Gladstone Peter Olupot-Olupot Joseph Ngonzi Drucilla J. Roberts Frederick A. Meier Rafael A. Irizarry James R. Broach Steven J. Schiff Joseph N. Paulson |
| author_facet |
Mercedeh Movassagh Lisa M. Bebell Kathy Burgoine Christine Hehnly Lijun Zhang Kim Moran Kathryn Sheldon Shamim A. Sinnar Edith Mbabazi-Kabachelor Elias Kumbakumba Joel Bazira Moses Ochora Ronnie Mulondo Brian Kaaya Nsubuga Andrew D. Weeks Melissa Gladstone Peter Olupot-Olupot Joseph Ngonzi Drucilla J. Roberts Frederick A. Meier Rafael A. Irizarry James R. Broach Steven J. Schiff Joseph N. Paulson |
| author_sort |
Mercedeh Movassagh |
| title |
Vaginal microbiome topic modeling of laboring Ugandan women with and without fever |
| title_short |
Vaginal microbiome topic modeling of laboring Ugandan women with and without fever |
| title_full |
Vaginal microbiome topic modeling of laboring Ugandan women with and without fever |
| title_fullStr |
Vaginal microbiome topic modeling of laboring Ugandan women with and without fever |
| title_full_unstemmed |
Vaginal microbiome topic modeling of laboring Ugandan women with and without fever |
| title_sort |
vaginal microbiome topic modeling of laboring ugandan women with and without fever |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/aec5a945eb0b423e9ee84dede5fe278d |
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