Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants

Rajendran Nithya, Nachiappan Meenakshi Sundaram Department of Biomedical Engineering, PSG College of Technology, Coimbatore, India Introduction: In recent years there has been a steep increase in the number of orthopedic patients for many reasons. One major reason is osteomyelitis, caused by pyrog...

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Autores principales:	Nithya R, Meenakshi Sundaram N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/aec88d1f56c749168efe104954647cb0
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spelling	oai:doaj.org-article:aec88d1f56c749168efe104954647cb02021-12-02T01:50:38ZBiodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants1178-2013https://doaj.org/article/aec88d1f56c749168efe104954647cb02015-10-01T00:00:00Zhttps://www.dovepress.com/biodegradation-and-cytotoxicity-of-ciprofloxacin-loaded-hydroxyapatite-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rajendran Nithya, Nachiappan Meenakshi Sundaram Department of Biomedical Engineering, PSG College of Technology, Coimbatore, India Introduction: In recent years there has been a steep increase in the number of orthopedic patients for many reasons. One major reason is osteomyelitis, caused by pyrogenic bacteria, with progressive infection of the bone or bone marrow and surrounding tissues. So antibiotics must be introduced during bone implantation to avoid prolonged infection.Aim: The objective of the study reported here was to prepare a composite film of nanocrystalline hydroxyapatite (HAp) and polycaprolactone (PCL) polymer loaded with ciprofloxacin, a frequently used antibiotic agent for bone infections.Methods: Nanocrystalline HAp was synthesized by precipitation method using the precursor obtained from eggshell. The nanocomposite film (HAp-PCL-ciprofloxacin) was prepared by solvent evaporation. Drug-release and biodegradation studies were undertaken by immersing the composite film in phosphate-buffered saline solution, while a cytotoxicity test was performed using the fibroblast cell line NIH-3T3 and osteoblast cell line MG-63.Results: The pure PCL film had quite a low dissolution rate after an initial sharp weight loss, whereas the ciprofloxacin-loaded HAp-PCL nanocomposite film had a large weight loss due to its fast drug release. The composite film had higher water absorption than the pure PCL, and increasing the concentration of the HAp increased the water absorption. The in vitro cell-line study showed a good biocompatibility and bioactivity of the developed nanocomposite film.Conclusion: The prepared film will act as a sustainable bone implant in addition to controlled drug delivery. Keywords: osteomyelitis, antibiotics, nanocrystalline, cytotoxicity, fibroblast, in vitroNithya RMeenakshi Sundaram NDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Supplement 1 Challenges in biomaterials research, Pp 119-127 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Nithya R Meenakshi Sundaram N Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants
description	Rajendran Nithya, Nachiappan Meenakshi Sundaram Department of Biomedical Engineering, PSG College of Technology, Coimbatore, India Introduction: In recent years there has been a steep increase in the number of orthopedic patients for many reasons. One major reason is osteomyelitis, caused by pyrogenic bacteria, with progressive infection of the bone or bone marrow and surrounding tissues. So antibiotics must be introduced during bone implantation to avoid prolonged infection.Aim: The objective of the study reported here was to prepare a composite film of nanocrystalline hydroxyapatite (HAp) and polycaprolactone (PCL) polymer loaded with ciprofloxacin, a frequently used antibiotic agent for bone infections.Methods: Nanocrystalline HAp was synthesized by precipitation method using the precursor obtained from eggshell. The nanocomposite film (HAp-PCL-ciprofloxacin) was prepared by solvent evaporation. Drug-release and biodegradation studies were undertaken by immersing the composite film in phosphate-buffered saline solution, while a cytotoxicity test was performed using the fibroblast cell line NIH-3T3 and osteoblast cell line MG-63.Results: The pure PCL film had quite a low dissolution rate after an initial sharp weight loss, whereas the ciprofloxacin-loaded HAp-PCL nanocomposite film had a large weight loss due to its fast drug release. The composite film had higher water absorption than the pure PCL, and increasing the concentration of the HAp increased the water absorption. The in vitro cell-line study showed a good biocompatibility and bioactivity of the developed nanocomposite film.Conclusion: The prepared film will act as a sustainable bone implant in addition to controlled drug delivery. Keywords: osteomyelitis, antibiotics, nanocrystalline, cytotoxicity, fibroblast, in vitro
format	article
author	Nithya R Meenakshi Sundaram N
author_facet	Nithya R Meenakshi Sundaram N
author_sort	Nithya R
title	Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants
title_short	Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants
title_full	Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants
title_fullStr	Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants
title_full_unstemmed	Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants
title_sort	biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/aec88d1f56c749168efe104954647cb0
work_keys_str_mv	AT nithyar biodegradationandcytotoxicityofciprofloxacinloadedhydroxyapatitepolycaprolactonenanocompositefilmforsustainableboneimplants AT meenakshisundaramn biodegradationandcytotoxicityofciprofloxacinloadedhydroxyapatitepolycaprolactonenanocompositefilmforsustainableboneimplants
_version_	1718402861315915776

Biodegradation and cytotoxicity of ciprofloxacin-loaded hydroxyapatite-polycaprolactone nanocomposite film for sustainable bone implants

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