ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.

<h4>Objective</h4>To develop a new ImmunoFISH technique for the study of oligodendrogliomas by combining a standard immunohistochemical stain using MIB-1 antibody with a standard FISH technique using commercial 1p36 and 19q13 chromosomal probes.<h4>Methods</h4>Validation was...

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Autores principales:	Céline Duval, Marie de Tayrac, François Sanschagrin, Karine Michaud, Peter Vincent Gould, Stéphan Saikali
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aec968e196264c38ba61ba94ec06b517
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spelling	oai:doaj.org-article:aec968e196264c38ba61ba94ec06b5172021-11-18T08:14:50ZImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.1932-620310.1371/journal.pone.0100342https://doaj.org/article/aec968e196264c38ba61ba94ec06b5172014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24949947/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>To develop a new ImmunoFISH technique for the study of oligodendrogliomas by combining a standard immunohistochemical stain using MIB-1 antibody with a standard FISH technique using commercial 1p36 and 19q13 chromosomal probes.<h4>Methods</h4>Validation was performed by two observers on a series of 36 pre-selected oligodendrogliomas and compared to the results previously determined by FISH alone.<h4>Results</h4>The ImFISH technique is easy to perform and to analyze and is no more time-consuming than the usual FISH technique. Our results show that the inter-observer reliability of ImFISH is high (κ = 0.86 and 0.95 respectively for 1p and 19q). Compared to FISH, the ImFISH exhibits a very high sensitivity (∼100%) and specificity (∼90%) for 1p and/or 19q deleted cases. The sensitivity is high for normal cases (∼85%) and imbalanced cases (∼90%) with a specificity ranging between 50 and 85%. Finally, there were no significant differences between FISH and ImFISH results calculated on 60, 40 or 20 cells.<h4>Conclusion</h4>Our study demonstrates the reliability of the ImFISH technique in oligodendrogliomas and emphasizes its advantage in poorly cellular tumoral specimen.Céline DuvalMarie de TayracFrançois SanschagrinKarine MichaudPeter Vincent GouldStéphan SaikaliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100342 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Céline Duval Marie de Tayrac François Sanschagrin Karine Michaud Peter Vincent Gould Stéphan Saikali ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.
description	<h4>Objective</h4>To develop a new ImmunoFISH technique for the study of oligodendrogliomas by combining a standard immunohistochemical stain using MIB-1 antibody with a standard FISH technique using commercial 1p36 and 19q13 chromosomal probes.<h4>Methods</h4>Validation was performed by two observers on a series of 36 pre-selected oligodendrogliomas and compared to the results previously determined by FISH alone.<h4>Results</h4>The ImFISH technique is easy to perform and to analyze and is no more time-consuming than the usual FISH technique. Our results show that the inter-observer reliability of ImFISH is high (κ = 0.86 and 0.95 respectively for 1p and 19q). Compared to FISH, the ImFISH exhibits a very high sensitivity (∼100%) and specificity (∼90%) for 1p and/or 19q deleted cases. The sensitivity is high for normal cases (∼85%) and imbalanced cases (∼90%) with a specificity ranging between 50 and 85%. Finally, there were no significant differences between FISH and ImFISH results calculated on 60, 40 or 20 cells.<h4>Conclusion</h4>Our study demonstrates the reliability of the ImFISH technique in oligodendrogliomas and emphasizes its advantage in poorly cellular tumoral specimen.
format	article
author	Céline Duval Marie de Tayrac François Sanschagrin Karine Michaud Peter Vincent Gould Stéphan Saikali
author_facet	Céline Duval Marie de Tayrac François Sanschagrin Karine Michaud Peter Vincent Gould Stéphan Saikali
author_sort	Céline Duval
title	ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.
title_short	ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.
title_full	ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.
title_fullStr	ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.
title_full_unstemmed	ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.
title_sort	immunofish is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/aec968e196264c38ba61ba94ec06b517
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ImmunoFISH is a reliable technique for the assessment of 1p and 19q status in oligodendrogliomas.

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