Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine

Abstract In Europe, Ixodes ricinus is the most important vector of human infectious diseases, most notably Lyme borreliosis and tick-borne encephalitis virus. Multiple non-natural hosts of I. ricinus have shown to develop immunity after repeated tick bites. Tick immunity has also been shown to impai...

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Autores principales:	Jos J. A. Trentelman, Julen Tomás-Cortázar, Sarah Knorr, Diego Barriales, Ondrej Hajdusek, Radek Sima, Jasmin I. Ersoz, Sukanya Narasimhan, Erol Fikrig, Ard M. Nijhof, Juan Anguita, Joppe W. Hovius
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aecc1fe1ce21426d9843e8b3495ae2c8
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spelling	oai:doaj.org-article:aecc1fe1ce21426d9843e8b3495ae2c82021-12-02T16:36:36ZProbing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine10.1038/s41598-021-92538-92045-2322https://doaj.org/article/aecc1fe1ce21426d9843e8b3495ae2c82021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92538-9https://doaj.org/toc/2045-2322Abstract In Europe, Ixodes ricinus is the most important vector of human infectious diseases, most notably Lyme borreliosis and tick-borne encephalitis virus. Multiple non-natural hosts of I. ricinus have shown to develop immunity after repeated tick bites. Tick immunity has also been shown to impair B. burgdorferi transmission. Most interestingly, multiple tick bites reduced the likelihood of contracting Lyme borreliosis in humans. A vaccine that mimics tick immunity could therefore potentially prevent Lyme borreliosis in humans. A yeast surface display library (YSD) of nymphal I. ricinus salivary gland genes expressed at 24, 48 and 72 h into tick feeding was constructed and probed with antibodies from humans repeatedly bitten by ticks, identifying twelve immunoreactive tick salivary gland proteins (TSGPs). From these, three proteins were selected for vaccination studies. An exploratory vaccination study in cattle showed an anti-tick effect when all three antigens were combined. However, immunization of rabbits did not provide equivalent levels of protection. Our results show that YSD is a powerful tool to identify immunodominant antigens in humans exposed to tick bites, yet vaccination with the three selected TSGPs did not provide protection in the present form. Future efforts will focus on exploring the biological functions of these proteins, consider alternative systems for recombinant protein generation and vaccination platforms and assess the potential of the other identified immunogenic TSGPs.Jos J. A. TrentelmanJulen Tomás-CortázarSarah KnorrDiego BarrialesOndrej HajdusekRadek SimaJasmin I. ErsozSukanya NarasimhanErol FikrigArd M. NijhofJuan AnguitaJoppe W. HoviusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jos J. A. Trentelman Julen Tomás-Cortázar Sarah Knorr Diego Barriales Ondrej Hajdusek Radek Sima Jasmin I. Ersoz Sukanya Narasimhan Erol Fikrig Ard M. Nijhof Juan Anguita Joppe W. Hovius Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine
description	Abstract In Europe, Ixodes ricinus is the most important vector of human infectious diseases, most notably Lyme borreliosis and tick-borne encephalitis virus. Multiple non-natural hosts of I. ricinus have shown to develop immunity after repeated tick bites. Tick immunity has also been shown to impair B. burgdorferi transmission. Most interestingly, multiple tick bites reduced the likelihood of contracting Lyme borreliosis in humans. A vaccine that mimics tick immunity could therefore potentially prevent Lyme borreliosis in humans. A yeast surface display library (YSD) of nymphal I. ricinus salivary gland genes expressed at 24, 48 and 72 h into tick feeding was constructed and probed with antibodies from humans repeatedly bitten by ticks, identifying twelve immunoreactive tick salivary gland proteins (TSGPs). From these, three proteins were selected for vaccination studies. An exploratory vaccination study in cattle showed an anti-tick effect when all three antigens were combined. However, immunization of rabbits did not provide equivalent levels of protection. Our results show that YSD is a powerful tool to identify immunodominant antigens in humans exposed to tick bites, yet vaccination with the three selected TSGPs did not provide protection in the present form. Future efforts will focus on exploring the biological functions of these proteins, consider alternative systems for recombinant protein generation and vaccination platforms and assess the potential of the other identified immunogenic TSGPs.
format	article
author	Jos J. A. Trentelman Julen Tomás-Cortázar Sarah Knorr Diego Barriales Ondrej Hajdusek Radek Sima Jasmin I. Ersoz Sukanya Narasimhan Erol Fikrig Ard M. Nijhof Juan Anguita Joppe W. Hovius
author_facet	Jos J. A. Trentelman Julen Tomás-Cortázar Sarah Knorr Diego Barriales Ondrej Hajdusek Radek Sima Jasmin I. Ersoz Sukanya Narasimhan Erol Fikrig Ard M. Nijhof Juan Anguita Joppe W. Hovius
author_sort	Jos J. A. Trentelman
title	Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine
title_short	Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine
title_full	Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine
title_fullStr	Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine
title_full_unstemmed	Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine
title_sort	probing an ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aecc1fe1ce21426d9843e8b3495ae2c8
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Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine

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