Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells

Abstract Background Human plasmacytoid dendritic cells (pDC) have a dual role as interferon-producing and antigen-presenting cells. Their relevance for allergic diseases is controversial. and the impact of pDC on allergic immune responses is poorly understood. Methods This in vitro study on human pD...

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Autores principales:	Noelle Zurmühl, Anna Schmitt, Ulrike Formentini, Johannes Weiss, Heike Appel, Klaus-Michael Debatin, Dorit Fabricius
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Plasmacytoid Dendritic cell Allergen uptake Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/aed766a16467466bbbcae64ff51f0bc6
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spelling	oai:doaj.org-article:aed766a16467466bbbcae64ff51f0bc62021-11-21T12:25:37ZDifferential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells10.1186/s12948-021-00163-81476-7961https://doaj.org/article/aed766a16467466bbbcae64ff51f0bc62021-11-01T00:00:00Zhttps://doi.org/10.1186/s12948-021-00163-8https://doaj.org/toc/1476-7961Abstract Background Human plasmacytoid dendritic cells (pDC) have a dual role as interferon-producing and antigen-presenting cells. Their relevance for allergic diseases is controversial. and the impact of pDC on allergic immune responses is poorly understood. Methods This in vitro study on human pDC isolated from peripheral blood was designed to compare side by side the uptake of three clinically relevant representative allergens: fluorochrome-labeled house dust mite Der p 1, Bee venom extract from Apis mellifera (Api) and the food allergen OVA analyzed flow cytometry and confocal microscopy. Results We found that the internalization and its regulation by TLR9 ligation was significantly different between allergens in terms of time course and strength of uptake. Api and OVA uptake in pDC of healthy subjects was faster and reached higher levels than Der p 1 uptake. CpG ODN 2006 suppressed OVA uptake and to a lesser extent Der p 1, while Api internalization was not affected. All allergens colocalized with LAMP1 and EEA1, with Api being internalized particularly fast and reaching highest intracellular levels in pDC. Of note, we could not determine any specific differences in antigen uptake in allergic compared with healthy subjects. Conclusions To our knowledge this is the first study that directly compares uptake regulation of clinically relevant inhalative, injective and food allergens in pDC. Our findings may help to explain differences in the onset and severity of allergic reactions as well as in the efficiency of AIT.Noelle ZurmühlAnna SchmittUlrike FormentiniJohannes WeissHeike AppelKlaus-Michael DebatinDorit FabriciusBMCarticlePlasmacytoidDendritic cellAllergen uptakeImmunologic diseases. AllergyRC581-607ENClinical and Molecular Allergy, Vol 19, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Plasmacytoid Dendritic cell Allergen uptake Immunologic diseases. Allergy RC581-607
spellingShingle	Plasmacytoid Dendritic cell Allergen uptake Immunologic diseases. Allergy RC581-607 Noelle Zurmühl Anna Schmitt Ulrike Formentini Johannes Weiss Heike Appel Klaus-Michael Debatin Dorit Fabricius Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells
description	Abstract Background Human plasmacytoid dendritic cells (pDC) have a dual role as interferon-producing and antigen-presenting cells. Their relevance for allergic diseases is controversial. and the impact of pDC on allergic immune responses is poorly understood. Methods This in vitro study on human pDC isolated from peripheral blood was designed to compare side by side the uptake of three clinically relevant representative allergens: fluorochrome-labeled house dust mite Der p 1, Bee venom extract from Apis mellifera (Api) and the food allergen OVA analyzed flow cytometry and confocal microscopy. Results We found that the internalization and its regulation by TLR9 ligation was significantly different between allergens in terms of time course and strength of uptake. Api and OVA uptake in pDC of healthy subjects was faster and reached higher levels than Der p 1 uptake. CpG ODN 2006 suppressed OVA uptake and to a lesser extent Der p 1, while Api internalization was not affected. All allergens colocalized with LAMP1 and EEA1, with Api being internalized particularly fast and reaching highest intracellular levels in pDC. Of note, we could not determine any specific differences in antigen uptake in allergic compared with healthy subjects. Conclusions To our knowledge this is the first study that directly compares uptake regulation of clinically relevant inhalative, injective and food allergens in pDC. Our findings may help to explain differences in the onset and severity of allergic reactions as well as in the efficiency of AIT.
format	article
author	Noelle Zurmühl Anna Schmitt Ulrike Formentini Johannes Weiss Heike Appel Klaus-Michael Debatin Dorit Fabricius
author_facet	Noelle Zurmühl Anna Schmitt Ulrike Formentini Johannes Weiss Heike Appel Klaus-Michael Debatin Dorit Fabricius
author_sort	Noelle Zurmühl
title	Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells
title_short	Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells
title_full	Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells
title_fullStr	Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells
title_full_unstemmed	Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells
title_sort	differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells
publisher	BMC
publishDate	2021
url	https://doaj.org/article/aed766a16467466bbbcae64ff51f0bc6
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Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells

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