Alteration of the gut microbiota in Chinese population with chronic kidney disease
Abstract We evaluated differences in the compositions of faecal microbiota between 52 end stage renal disease (ESRD) patients and 60 healthy controls in southern China using quantitative real-time polymerase chain reaction (qPCR) and high-throughput sequencing (16S ribosomal RNA V4-6 region) methods...
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Nature Portfolio
2017
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oai:doaj.org-article:aedb9f1e9b1c4b01b35c5708d031d8182021-12-02T11:51:13ZAlteration of the gut microbiota in Chinese population with chronic kidney disease10.1038/s41598-017-02989-22045-2322https://doaj.org/article/aedb9f1e9b1c4b01b35c5708d031d8182017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02989-2https://doaj.org/toc/2045-2322Abstract We evaluated differences in the compositions of faecal microbiota between 52 end stage renal disease (ESRD) patients and 60 healthy controls in southern China using quantitative real-time polymerase chain reaction (qPCR) and high-throughput sequencing (16S ribosomal RNA V4-6 region) methods. The absolute quantification of total bacteria was significantly reduced in ESRD patients (p < 0.01). In three enterotypes, Prevotella was enriched in the healthy group whereas Bacteroides were prevalent in the ESRD group (LDA score > 4.5). 11 bacterial taxa were significantly overrepresented in samples from ESRD and 22 bacterial taxa were overrepresented in samples from healthy controls. The butyrate producing bacteria, Roseburia, Faecalibacterium, Clostridium, Coprococcus and Prevotella were reduced in the ESRD group (LDA values > 2.0). Canonical correspondence analysis (CCA) indicated that Cystatin C (CysC), creatinine and eGFR appeared to be the most important environmental parameters to influence the overall microbial communities. In qPCR analysis, The butyrate producing species Roseburia spp., Faecalibacterium prausnitzii, Prevotella and Universal bacteria, were negatively related to CRP and CysC. Total bacteria in faeces were reduced in patients with ESRD compared to that in healthy individuals. The enterotypes change from Prevotella to Bacteroides in ESRD patients. The gut microbiota was associated with the inflammatory state and renal function of chronic kidney disease.Shuanghong JiangShan XieDan LvPu WangHanchang HeTing ZhangYoulian ZhouQianyun LinHongwei ZhouJianping JiangJing NieFanfan HouYe ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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Medicine R Science Q Shuanghong Jiang Shan Xie Dan Lv Pu Wang Hanchang He Ting Zhang Youlian Zhou Qianyun Lin Hongwei Zhou Jianping Jiang Jing Nie Fanfan Hou Ye Chen Alteration of the gut microbiota in Chinese population with chronic kidney disease |
| description |
Abstract We evaluated differences in the compositions of faecal microbiota between 52 end stage renal disease (ESRD) patients and 60 healthy controls in southern China using quantitative real-time polymerase chain reaction (qPCR) and high-throughput sequencing (16S ribosomal RNA V4-6 region) methods. The absolute quantification of total bacteria was significantly reduced in ESRD patients (p < 0.01). In three enterotypes, Prevotella was enriched in the healthy group whereas Bacteroides were prevalent in the ESRD group (LDA score > 4.5). 11 bacterial taxa were significantly overrepresented in samples from ESRD and 22 bacterial taxa were overrepresented in samples from healthy controls. The butyrate producing bacteria, Roseburia, Faecalibacterium, Clostridium, Coprococcus and Prevotella were reduced in the ESRD group (LDA values > 2.0). Canonical correspondence analysis (CCA) indicated that Cystatin C (CysC), creatinine and eGFR appeared to be the most important environmental parameters to influence the overall microbial communities. In qPCR analysis, The butyrate producing species Roseburia spp., Faecalibacterium prausnitzii, Prevotella and Universal bacteria, were negatively related to CRP and CysC. Total bacteria in faeces were reduced in patients with ESRD compared to that in healthy individuals. The enterotypes change from Prevotella to Bacteroides in ESRD patients. The gut microbiota was associated with the inflammatory state and renal function of chronic kidney disease. |
| format |
article |
| author |
Shuanghong Jiang Shan Xie Dan Lv Pu Wang Hanchang He Ting Zhang Youlian Zhou Qianyun Lin Hongwei Zhou Jianping Jiang Jing Nie Fanfan Hou Ye Chen |
| author_facet |
Shuanghong Jiang Shan Xie Dan Lv Pu Wang Hanchang He Ting Zhang Youlian Zhou Qianyun Lin Hongwei Zhou Jianping Jiang Jing Nie Fanfan Hou Ye Chen |
| author_sort |
Shuanghong Jiang |
| title |
Alteration of the gut microbiota in Chinese population with chronic kidney disease |
| title_short |
Alteration of the gut microbiota in Chinese population with chronic kidney disease |
| title_full |
Alteration of the gut microbiota in Chinese population with chronic kidney disease |
| title_fullStr |
Alteration of the gut microbiota in Chinese population with chronic kidney disease |
| title_full_unstemmed |
Alteration of the gut microbiota in Chinese population with chronic kidney disease |
| title_sort |
alteration of the gut microbiota in chinese population with chronic kidney disease |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/aedb9f1e9b1c4b01b35c5708d031d818 |
| work_keys_str_mv |
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1718395185657806848 |