Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.

The present study aimed to identify and characterize plasmids in a national collection of oral Enterococcus faecalis (n = 106) isolated from patients with marginal periodontitis. Plasmid replicon typing was performed by multiplex-PCR and sequencing with specific primers for 18 rep-families and 1 uni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaobo Song, Jinglu Sun, Theresa Mikalsen, Adam P Roberts, Arnfinn Sundsfjord
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aedef48709624154a64945146f5838a8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:aedef48709624154a64945146f5838a8
record_format	dspace
spelling	oai:doaj.org-article:aedef48709624154a64945146f5838a82021-11-18T07:47:25ZCharacterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.1932-620310.1371/journal.pone.0062248https://doaj.org/article/aedef48709624154a64945146f5838a82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23646122/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The present study aimed to identify and characterize plasmids in a national collection of oral Enterococcus faecalis (n = 106) isolated from patients with marginal periodontitis. Plasmid replicon typing was performed by multiplex-PCR and sequencing with specific primers for 18 rep-families and 1 unique sequence. Additional plasmid analysis by S1-PFGE was performed for comparison. Totally 120 plasmid replicon amplicons of seven rep-families were identified in 93 E. faecalis strains, e.g. rep9 (prototype pCF10), rep6 (prototype pS86), rep2 (prototype pRE25/pEF1), and rep8 (prototype pAM373). Rep9 was the most predominant rep-family being detected in 81 (76.4%) strains. Forty of these strains were tetracycline resistant and three were erythromycin resistant. Rep6 was the second predominant rep-family being detected in 22 (20.8%) strains. Rep2 was detected in eight (7.5%) strains. All rep2-positive strains were resistant to tetracycline and/or erythromycin and six of them contained Tn916/Tn1545 genes. The rep-positive E. faecalis exhibited divergence in multilocus sequence types (STs). There was a significant correlation between rep9 and ST21, while multiple rep-families appeared in ST40. Totally 145 plasmid bands were identified in 95 E. faecalis strains by S1-PFGE, 59 strains carrying one plasmid, 27 carrying two, five carrying three, three carrying four, and one strain carrying five plasmids. Plasmid sizes varied between 5-150 kbp. There was a significant correlation between the number of plasmids identified by PCR rep-typing and by S1-PFGE. The results indicate that the majority of E. faecalis of marginal periodontitis are likely to be a reservoir for diverse mobile genetic elements and associated antimicrobial resistance determinants.Xiaobo SongJinglu SunTheresa MikalsenAdam P RobertsArnfinn SundsfjordPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62248 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaobo Song Jinglu Sun Theresa Mikalsen Adam P Roberts Arnfinn Sundsfjord Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.
description	The present study aimed to identify and characterize plasmids in a national collection of oral Enterococcus faecalis (n = 106) isolated from patients with marginal periodontitis. Plasmid replicon typing was performed by multiplex-PCR and sequencing with specific primers for 18 rep-families and 1 unique sequence. Additional plasmid analysis by S1-PFGE was performed for comparison. Totally 120 plasmid replicon amplicons of seven rep-families were identified in 93 E. faecalis strains, e.g. rep9 (prototype pCF10), rep6 (prototype pS86), rep2 (prototype pRE25/pEF1), and rep8 (prototype pAM373). Rep9 was the most predominant rep-family being detected in 81 (76.4%) strains. Forty of these strains were tetracycline resistant and three were erythromycin resistant. Rep6 was the second predominant rep-family being detected in 22 (20.8%) strains. Rep2 was detected in eight (7.5%) strains. All rep2-positive strains were resistant to tetracycline and/or erythromycin and six of them contained Tn916/Tn1545 genes. The rep-positive E. faecalis exhibited divergence in multilocus sequence types (STs). There was a significant correlation between rep9 and ST21, while multiple rep-families appeared in ST40. Totally 145 plasmid bands were identified in 95 E. faecalis strains by S1-PFGE, 59 strains carrying one plasmid, 27 carrying two, five carrying three, three carrying four, and one strain carrying five plasmids. Plasmid sizes varied between 5-150 kbp. There was a significant correlation between the number of plasmids identified by PCR rep-typing and by S1-PFGE. The results indicate that the majority of E. faecalis of marginal periodontitis are likely to be a reservoir for diverse mobile genetic elements and associated antimicrobial resistance determinants.
format	article
author	Xiaobo Song Jinglu Sun Theresa Mikalsen Adam P Roberts Arnfinn Sundsfjord
author_facet	Xiaobo Song Jinglu Sun Theresa Mikalsen Adam P Roberts Arnfinn Sundsfjord
author_sort	Xiaobo Song
title	Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.
title_short	Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.
title_full	Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.
title_fullStr	Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.
title_full_unstemmed	Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.
title_sort	characterisation of the plasmidome within enterococcus faecalis isolated from marginal periodontitis patients in norway.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/aedef48709624154a64945146f5838a8
work_keys_str_mv	AT xiaobosong characterisationoftheplasmidomewithinenterococcusfaecalisisolatedfrommarginalperiodontitispatientsinnorway AT jinglusun characterisationoftheplasmidomewithinenterococcusfaecalisisolatedfrommarginalperiodontitispatientsinnorway AT theresamikalsen characterisationoftheplasmidomewithinenterococcusfaecalisisolatedfrommarginalperiodontitispatientsinnorway AT adamproberts characterisationoftheplasmidomewithinenterococcusfaecalisisolatedfrommarginalperiodontitispatientsinnorway AT arnfinnsundsfjord characterisationoftheplasmidomewithinenterococcusfaecalisisolatedfrommarginalperiodontitispatientsinnorway
_version_	1718422994486820864

Characterisation of the plasmidome within Enterococcus faecalis isolated from marginal periodontitis patients in Norway.

Ejemplares similares