Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells

Detecting proteins and post-translational modifications is important for drug screens, but the number of proteins measurable simultaneously is limited. Here the authors use antibodies tagged with DNA barcodes and high-throughput sequencing to detect up to 70 (phospho-)proteins in stem cells.

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Autores principales:	Jessie A. G. van Buggenum, Jan P. Gerlach, Sabine E. J. Tanis, Mark Hogeweg, Pascal W. T. C. Jansen, Jesse Middelwijk, Ruud van der Steen, Michiel Vermeulen, Hendrik G. Stunnenberg, Cornelis A. Albers, Klaas W. Mulder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/aee29dc4444b4d96b9ebc997bd57935e
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spelling	oai:doaj.org-article:aee29dc4444b4d96b9ebc997bd57935e2021-12-02T15:34:40ZImmuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells10.1038/s41467-018-04761-02041-1723https://doaj.org/article/aee29dc4444b4d96b9ebc997bd57935e2018-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04761-0https://doaj.org/toc/2041-1723Detecting proteins and post-translational modifications is important for drug screens, but the number of proteins measurable simultaneously is limited. Here the authors use antibodies tagged with DNA barcodes and high-throughput sequencing to detect up to 70 (phospho-)proteins in stem cells.Jessie A. G. van BuggenumJan P. GerlachSabine E. J. TanisMark HogewegPascal W. T. C. JansenJesse MiddelwijkRuud van der SteenMichiel VermeulenHendrik G. StunnenbergCornelis A. AlbersKlaas W. MulderNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jessie A. G. van Buggenum Jan P. Gerlach Sabine E. J. Tanis Mark Hogeweg Pascal W. T. C. Jansen Jesse Middelwijk Ruud van der Steen Michiel Vermeulen Hendrik G. Stunnenberg Cornelis A. Albers Klaas W. Mulder Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells
description	Detecting proteins and post-translational modifications is important for drug screens, but the number of proteins measurable simultaneously is limited. Here the authors use antibodies tagged with DNA barcodes and high-throughput sequencing to detect up to 70 (phospho-)proteins in stem cells.
format	article
author	Jessie A. G. van Buggenum Jan P. Gerlach Sabine E. J. Tanis Mark Hogeweg Pascal W. T. C. Jansen Jesse Middelwijk Ruud van der Steen Michiel Vermeulen Hendrik G. Stunnenberg Cornelis A. Albers Klaas W. Mulder
author_facet	Jessie A. G. van Buggenum Jan P. Gerlach Sabine E. J. Tanis Mark Hogeweg Pascal W. T. C. Jansen Jesse Middelwijk Ruud van der Steen Michiel Vermeulen Hendrik G. Stunnenberg Cornelis A. Albers Klaas W. Mulder
author_sort	Jessie A. G. van Buggenum
title	Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells
title_short	Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells
title_full	Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells
title_fullStr	Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells
title_full_unstemmed	Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells
title_sort	immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/aee29dc4444b4d96b9ebc997bd57935e
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Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells

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