Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.

Electrical stimulation is one of the candidates for elongation-driven regeneration of damaged peripheral nerves. Different organs and tissues have an inherent cell structure and size. This leads to variation in the tissue-specific electrical properties of the frequency of interfacial polarization. A...

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Autores principales:	Risa Otagiri, Hideki Kawai, Masanobu Takatsuka, Naoki Shinyashiki, Akira Ito, Ryosuke Ikeguchi, Tomoki Aoyama
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aee56febd9834245b022eedd6759b9c7
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spelling	oai:doaj.org-article:aee56febd9834245b022eedd6759b9c72021-12-02T20:11:09ZInterfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.1932-620310.1371/journal.pone.0252589https://doaj.org/article/aee56febd9834245b022eedd6759b9c72021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252589https://doaj.org/toc/1932-6203Electrical stimulation is one of the candidates for elongation-driven regeneration of damaged peripheral nerves. Different organs and tissues have an inherent cell structure and size. This leads to variation in the tissue-specific electrical properties of the frequency of interfacial polarization. Although nervous tissues have a membrane potential, the electrical reaction inside these tissues following electrical stimulation from outside remains unexplored. Furthermore, the pathophysiological reaction of an injured nerve is unclear. Here, we investigated the electrical reaction of injured and non-injured rat sciatic nerves via broadband dielectric spectroscopy. Crush injured and non-injured sciatic nerves of six 12-week-old male Lewis rats were used, 6 days after infliction of the injury. Both sides of the nerves (with and without injury) were exposed, and impedance measurements were performed at room temperature (approximately 25°C) at frequencies ranging from 100 mHz to 5.5 MHz and electric potential ranging from 0.100 to 1.00 V. The measured interfacial polarization potentially originated from the polarization by ion transport around nerve membranes at frequencies between 3.2 kHz and 1.6 MHz. The polarization strength of the injured nerves was smaller than that of non-injured nerves. However, the difference in polarization between injured and non-injured nerves might be caused by inflammation and edema. The suitable frequency range of the interfacial polarization can be expected to be critical for electrical stimulation of injured peripheral nerves.Risa OtagiriHideki KawaiMasanobu TakatsukaNaoki ShinyashikiAkira ItoRyosuke IkeguchiTomoki AoyamaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252589 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Risa Otagiri Hideki Kawai Masanobu Takatsuka Naoki Shinyashiki Akira Ito Ryosuke Ikeguchi Tomoki Aoyama Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.
description	Electrical stimulation is one of the candidates for elongation-driven regeneration of damaged peripheral nerves. Different organs and tissues have an inherent cell structure and size. This leads to variation in the tissue-specific electrical properties of the frequency of interfacial polarization. Although nervous tissues have a membrane potential, the electrical reaction inside these tissues following electrical stimulation from outside remains unexplored. Furthermore, the pathophysiological reaction of an injured nerve is unclear. Here, we investigated the electrical reaction of injured and non-injured rat sciatic nerves via broadband dielectric spectroscopy. Crush injured and non-injured sciatic nerves of six 12-week-old male Lewis rats were used, 6 days after infliction of the injury. Both sides of the nerves (with and without injury) were exposed, and impedance measurements were performed at room temperature (approximately 25°C) at frequencies ranging from 100 mHz to 5.5 MHz and electric potential ranging from 0.100 to 1.00 V. The measured interfacial polarization potentially originated from the polarization by ion transport around nerve membranes at frequencies between 3.2 kHz and 1.6 MHz. The polarization strength of the injured nerves was smaller than that of non-injured nerves. However, the difference in polarization between injured and non-injured nerves might be caused by inflammation and edema. The suitable frequency range of the interfacial polarization can be expected to be critical for electrical stimulation of injured peripheral nerves.
format	article
author	Risa Otagiri Hideki Kawai Masanobu Takatsuka Naoki Shinyashiki Akira Ito Ryosuke Ikeguchi Tomoki Aoyama
author_facet	Risa Otagiri Hideki Kawai Masanobu Takatsuka Naoki Shinyashiki Akira Ito Ryosuke Ikeguchi Tomoki Aoyama
author_sort	Risa Otagiri
title	Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.
title_short	Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.
title_full	Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.
title_fullStr	Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.
title_full_unstemmed	Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.
title_sort	interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/aee56febd9834245b022eedd6759b9c7
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Interfacial polarization of in vivo rat sciatic nerve with crush injury studied via broadband dielectric spectroscopy.

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