Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia

Abstract. Hidayaturrahmah, Mabrur, Santoso HB, Sasmita R, Rahmy USA, Badruzsaufari. 2019. Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia. Biodiversitas 20: 745-753. Giant mudskipper (Pe...

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Autores principales:	HIDAYATURRAHMAH HIDAYATURRAHMAH, MABRUR MABRUR, HERI BUDI SANTOSO, RANI SASMITA, UMMY SHALIHA AULIA RAHMY, BADRUZSAUFARI BADRUZSAUFARI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/aee8870101134a47b4ad410e389800d8
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spelling	oai:doaj.org-article:aee8870101134a47b4ad410e389800d82021-11-16T14:12:15ZShort Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia1412-033X2085-472210.13057/biodiv/d200319https://doaj.org/article/aee8870101134a47b4ad410e389800d82019-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3338https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Hidayaturrahmah, Mabrur, Santoso HB, Sasmita R, Rahmy USA, Badruzsaufari. 2019. Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia. Biodiversitas 20: 745-753. Giant mudskipper (Periphthalmodon schlosseri, Pallas 1770) is one of important biological diversity that potential as bio-indicator in environment assessment which is heavy metal contamination. These fish are susceptible to ambient environment, that effect to their physiological mechanism including protein synthesis and expression. This research aims to identify protein profiles of giant mudskipper muscle and review its potential application as a biomarker for heavy metal contamination in Barito Estuary of South Kalimantan. Total proteins were isolated from fish muscle using Tris EDTA buffer and then precipitated using Ammonium sulfate salt. The protein concentration was measured using Bradford assay and then separated based on molecular weight using the SDS-PAGE method. The result showed muscle proteins of giant mudskipper could be precipitated on optimum condition at Ammonium sulfate saturation 60-70% which protein concentration 5.106 mg.mL-1. The protein separated into 20 bands for raw and precipitated proteins which molecular weight ranging from 33 into 184 kDa. Further study is needed for protein identification based on amino acids sequence of protein bands for biomarker discovery and validation.HIDAYATURRAHMAH HIDAYATURRAHMAHMABRUR MABRURHERI BUDI SANTOSORANI SASMITAUMMY SHALIHA AULIA RAHMYBADRUZSAUFARI BADRUZSAUFARIMBI & UNS SoloarticleBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 3, Pp 745-753 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 HIDAYATURRAHMAH HIDAYATURRAHMAH MABRUR MABRUR HERI BUDI SANTOSO RANI SASMITA UMMY SHALIHA AULIA RAHMY BADRUZSAUFARI BADRUZSAUFARI Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia
description	Abstract. Hidayaturrahmah, Mabrur, Santoso HB, Sasmita R, Rahmy USA, Badruzsaufari. 2019. Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia. Biodiversitas 20: 745-753. Giant mudskipper (Periphthalmodon schlosseri, Pallas 1770) is one of important biological diversity that potential as bio-indicator in environment assessment which is heavy metal contamination. These fish are susceptible to ambient environment, that effect to their physiological mechanism including protein synthesis and expression. This research aims to identify protein profiles of giant mudskipper muscle and review its potential application as a biomarker for heavy metal contamination in Barito Estuary of South Kalimantan. Total proteins were isolated from fish muscle using Tris EDTA buffer and then precipitated using Ammonium sulfate salt. The protein concentration was measured using Bradford assay and then separated based on molecular weight using the SDS-PAGE method. The result showed muscle proteins of giant mudskipper could be precipitated on optimum condition at Ammonium sulfate saturation 60-70% which protein concentration 5.106 mg.mL-1. The protein separated into 20 bands for raw and precipitated proteins which molecular weight ranging from 33 into 184 kDa. Further study is needed for protein identification based on amino acids sequence of protein bands for biomarker discovery and validation.
format	article
author	HIDAYATURRAHMAH HIDAYATURRAHMAH MABRUR MABRUR HERI BUDI SANTOSO RANI SASMITA UMMY SHALIHA AULIA RAHMY BADRUZSAUFARI BADRUZSAUFARI
author_facet	HIDAYATURRAHMAH HIDAYATURRAHMAH MABRUR MABRUR HERI BUDI SANTOSO RANI SASMITA UMMY SHALIHA AULIA RAHMY BADRUZSAUFARI BADRUZSAUFARI
author_sort	HIDAYATURRAHMAH HIDAYATURRAHMAH
title	Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia
title_short	Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia
title_full	Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia
title_fullStr	Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia
title_full_unstemmed	Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia
title_sort	short communication: protein profiles of giant mudskipper and its potential use as biomarker candidate for heavy metal contamination in barito estuary, indonesia
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/aee8870101134a47b4ad410e389800d8
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Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia

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