Circulating miR-15a and miR-222 as Potential Biomarkers of Type 2 Diabetes

Salman Sadeghzadeh,1 Mahmood Dehghani Ashkezari,1 Seyed Morteza Seifati,1 Mohammad Yahya Vahidi Mehrjardi,2,3 Masoud Dehghan Tezerjani,4 Sara Sadeghzadeh,5 Seyed Amir Behtash Ladan1 1Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran; 2Diabetes Rese...

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Autores principales:	Sadeghzadeh S, Dehghani Ashkezari M, Seifati SM, Vahidi Mehrjardi MY, Dehghan Tezerjani M, Ladan SAB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	type 2 diabetes mir-15a mir-222 biomarker gene expression Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/aee9ace69b804bd1a6808cec9382cb71
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spelling	oai:doaj.org-article:aee9ace69b804bd1a6808cec9382cb712021-12-02T12:56:58ZCirculating miR-15a and miR-222 as Potential Biomarkers of Type 2 Diabetes1178-7007https://doaj.org/article/aee9ace69b804bd1a6808cec9382cb712020-10-01T00:00:00Zhttps://www.dovepress.com/circulating-mir-15a-and-mir-222-as-potential-biomarkers-of-type-2-diab-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Salman Sadeghzadeh,1 Mahmood Dehghani Ashkezari,1 Seyed Morteza Seifati,1 Mohammad Yahya Vahidi Mehrjardi,2,3 Masoud Dehghan Tezerjani,4 Sara Sadeghzadeh,5 Seyed Amir Behtash Ladan1 1Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran; 2Diabetes Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Genetics, Medical School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 4Abortion Research Center, Yazd Institute of Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 5Department of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranCorrespondence: Mahmood Dehghani AshkezariMedical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, IranEmail mdashkezary@gmail.comBackground: In recent years, considerable attention has been paid to the role of microRNAs (miRs) as biomarkers in type 2 diabetes (T2D). The aim of the study was to evaluate the expression levels of miR-15a and miR-222 in diabetic, pre-diabetic, and healthy individuals.Materials and Methods: Ninety individuals, who were referred to the Yazd diabetic center, were enrolled in this study and then classified into three groups as healthy, pre-T2D, and diabetic based on the clinical manifestations. Real-time PCR was performed to explore miRs expression in the plasma samples of the studied population. The correlation between the biochemical characteristic and the expression of these miRs as well as specificity and sensitivity of different clinical markers in healthy and pre-diabetic groups was evaluated.Results: miR-222 expression was significantly upregulated in the pre-T2D cases compared to the control subjects (P< 0.001), while no significant difference was found between the pre-T2D and T2D groups (P< 0.05). The expression of miR-15a was statistically downregulated in the pre-T2D and T2D subjects (P< 0.05). The receiver operating characteristic (ROC) curve analysis of miR-15a expression with a cutoff point of 1.12 resulted in the area under the curve (AUC) of 85% (95% CI 0.865– 0.912; P< 0.001) with 84% and 85% sensitivity and specificity, respectively. Similarly, for miR-222, the cutoff point of 4.03 and AUC of 86% (95% CI 0.875– 0.943; P< 0.001) discriminated against the pre-T2D and control subjects via the sensitivity and specificity of 86% and 87%, respectively. Moreover, miR-15a values showed a negative correlation with FG (R=− 0.32, P=0.005); however, miR-222 values were positively correlated with FG (R=0.25, P=0.03) in the pre-T2D group. Furthermore, miR-222 values were correlated with OGTT in the pre-T2D group (R=0.27, P=0.001). In addition, LDL-C had a negative correlation with miR-222 values in the pre-T2D group (R=− 0.23, P=0.02).Conclusion: This study indicated that the plasma expression levels of miR-222 and miR-15a can be considered as non-invasive, fast tools to separate the pre-T2D individuals from their healthy counterparts. Accordingly, this information could be used to predict the development of the disease as well as a direction for optimal therapy, thus refining outcomes in patients with diabetes.Keywords: type 2 diabetes, miR-15a, miR-222, biomarker, gene expressionSadeghzadeh SDehghani Ashkezari MSeifati SMVahidi Mehrjardi MYDehghan Tezerjani MSadeghzadeh SLadan SABDove Medical Pressarticletype 2 diabetesmir-15amir-222biomarkergene expressionSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 3461-3469 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	type 2 diabetes mir-15a mir-222 biomarker gene expression Specialties of internal medicine RC581-951
spellingShingle	type 2 diabetes mir-15a mir-222 biomarker gene expression Specialties of internal medicine RC581-951 Sadeghzadeh S Dehghani Ashkezari M Seifati SM Vahidi Mehrjardi MY Dehghan Tezerjani M Sadeghzadeh S Ladan SAB Circulating miR-15a and miR-222 as Potential Biomarkers of Type 2 Diabetes
description	Salman Sadeghzadeh,1 Mahmood Dehghani Ashkezari,1 Seyed Morteza Seifati,1 Mohammad Yahya Vahidi Mehrjardi,2,3 Masoud Dehghan Tezerjani,4 Sara Sadeghzadeh,5 Seyed Amir Behtash Ladan1 1Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran; 2Diabetes Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 3Department of Genetics, Medical School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 4Abortion Research Center, Yazd Institute of Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran; 5Department of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranCorrespondence: Mahmood Dehghani AshkezariMedical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, IranEmail mdashkezary@gmail.comBackground: In recent years, considerable attention has been paid to the role of microRNAs (miRs) as biomarkers in type 2 diabetes (T2D). The aim of the study was to evaluate the expression levels of miR-15a and miR-222 in diabetic, pre-diabetic, and healthy individuals.Materials and Methods: Ninety individuals, who were referred to the Yazd diabetic center, were enrolled in this study and then classified into three groups as healthy, pre-T2D, and diabetic based on the clinical manifestations. Real-time PCR was performed to explore miRs expression in the plasma samples of the studied population. The correlation between the biochemical characteristic and the expression of these miRs as well as specificity and sensitivity of different clinical markers in healthy and pre-diabetic groups was evaluated.Results: miR-222 expression was significantly upregulated in the pre-T2D cases compared to the control subjects (P< 0.001), while no significant difference was found between the pre-T2D and T2D groups (P< 0.05). The expression of miR-15a was statistically downregulated in the pre-T2D and T2D subjects (P< 0.05). The receiver operating characteristic (ROC) curve analysis of miR-15a expression with a cutoff point of 1.12 resulted in the area under the curve (AUC) of 85% (95% CI 0.865– 0.912; P< 0.001) with 84% and 85% sensitivity and specificity, respectively. Similarly, for miR-222, the cutoff point of 4.03 and AUC of 86% (95% CI 0.875– 0.943; P< 0.001) discriminated against the pre-T2D and control subjects via the sensitivity and specificity of 86% and 87%, respectively. Moreover, miR-15a values showed a negative correlation with FG (R=− 0.32, P=0.005); however, miR-222 values were positively correlated with FG (R=0.25, P=0.03) in the pre-T2D group. Furthermore, miR-222 values were correlated with OGTT in the pre-T2D group (R=0.27, P=0.001). In addition, LDL-C had a negative correlation with miR-222 values in the pre-T2D group (R=− 0.23, P=0.02).Conclusion: This study indicated that the plasma expression levels of miR-222 and miR-15a can be considered as non-invasive, fast tools to separate the pre-T2D individuals from their healthy counterparts. Accordingly, this information could be used to predict the development of the disease as well as a direction for optimal therapy, thus refining outcomes in patients with diabetes.Keywords: type 2 diabetes, miR-15a, miR-222, biomarker, gene expression
format	article
author	Sadeghzadeh S Dehghani Ashkezari M Seifati SM Vahidi Mehrjardi MY Dehghan Tezerjani M Sadeghzadeh S Ladan SAB
author_facet	Sadeghzadeh S Dehghani Ashkezari M Seifati SM Vahidi Mehrjardi MY Dehghan Tezerjani M Sadeghzadeh S Ladan SAB
author_sort	Sadeghzadeh S
title	Circulating miR-15a and miR-222 as Potential Biomarkers of Type 2 Diabetes
title_short	Circulating miR-15a and miR-222 as Potential Biomarkers of Type 2 Diabetes
title_full	Circulating miR-15a and miR-222 as Potential Biomarkers of Type 2 Diabetes
title_fullStr	Circulating miR-15a and miR-222 as Potential Biomarkers of Type 2 Diabetes
title_full_unstemmed	Circulating miR-15a and miR-222 as Potential Biomarkers of Type 2 Diabetes
title_sort	circulating mir-15a and mir-222 as potential biomarkers of type 2 diabetes
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/aee9ace69b804bd1a6808cec9382cb71
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Circulating miR-15a and miR-222 as Potential Biomarkers of Type 2 Diabetes

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