The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State

Macrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition, macrophages undergo activation toward specific functional states, consisting not only in the acquisition of specific features but also of peculia...

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Autores principales:	Iolanda Spera, Ricardo Sánchez-Rodríguez, Maria Favia, Alessio Menga, Francisca C. Venegas, Roberta Angioni, Fabio Munari, Martina Lanza, Annalisa Campanella, Ciro L. Pierri, Marcella Canton, Alessandra Castegna
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	macrophage immortalized macrophages metabolism inflammation immune model cell line Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/aeeb579382e4484f841d3b19c190ea7e
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spelling	oai:doaj.org-article:aeeb579382e4484f841d3b19c190ea7e2021-11-11T15:33:39ZThe J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State10.3390/cancers132154782072-6694https://doaj.org/article/aeeb579382e4484f841d3b19c190ea7e2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5478https://doaj.org/toc/2072-6694Macrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition, macrophages undergo activation toward specific functional states, consisting not only in the acquisition of specific features but also of peculiar metabolic programs associated with each function. For these reasons, macrophages are often isolated from mice to perform cellular assays to study the mechanisms mediating immune cell activation. This requires expensive and time-consuming breeding and housing of mice strains. To overcome this issue, we analyzed an in-house J2-generated immortalized macrophage cell line from BMDMs, both from a functional and metabolic point of view. By assaying the intracellular and extracellular metabolism coupled with the phenotypic features of immortalized versus primary BMDMs, we concluded that classically and alternatively immortalized macrophages display similar phenotypical, metabolic and functional features compared to primary cells polarized in the same way. Our study validates the use of this immortalized cell line as a suitable model with which to evaluate in vitro how perturbations can influence the phenotypical and functional features of murine macrophages.Iolanda SperaRicardo Sánchez-RodríguezMaria FaviaAlessio MengaFrancisca C. VenegasRoberta AngioniFabio MunariMartina LanzaAnnalisa CampanellaCiro L. PierriMarcella CantonAlessandra CastegnaMDPI AGarticlemacrophageimmortalized macrophagesmetabolisminflammationimmune modelcell lineNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5478, p 5478 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	macrophage immortalized macrophages metabolism inflammation immune model cell line Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	macrophage immortalized macrophages metabolism inflammation immune model cell line Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Iolanda Spera Ricardo Sánchez-Rodríguez Maria Favia Alessio Menga Francisca C. Venegas Roberta Angioni Fabio Munari Martina Lanza Annalisa Campanella Ciro L. Pierri Marcella Canton Alessandra Castegna The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State
description	Macrophages are immune cells that are important for the development of the defensive front line of the innate immune system. Following signal recognition, macrophages undergo activation toward specific functional states, consisting not only in the acquisition of specific features but also of peculiar metabolic programs associated with each function. For these reasons, macrophages are often isolated from mice to perform cellular assays to study the mechanisms mediating immune cell activation. This requires expensive and time-consuming breeding and housing of mice strains. To overcome this issue, we analyzed an in-house J2-generated immortalized macrophage cell line from BMDMs, both from a functional and metabolic point of view. By assaying the intracellular and extracellular metabolism coupled with the phenotypic features of immortalized versus primary BMDMs, we concluded that classically and alternatively immortalized macrophages display similar phenotypical, metabolic and functional features compared to primary cells polarized in the same way. Our study validates the use of this immortalized cell line as a suitable model with which to evaluate in vitro how perturbations can influence the phenotypical and functional features of murine macrophages.
format	article
author	Iolanda Spera Ricardo Sánchez-Rodríguez Maria Favia Alessio Menga Francisca C. Venegas Roberta Angioni Fabio Munari Martina Lanza Annalisa Campanella Ciro L. Pierri Marcella Canton Alessandra Castegna
author_facet	Iolanda Spera Ricardo Sánchez-Rodríguez Maria Favia Alessio Menga Francisca C. Venegas Roberta Angioni Fabio Munari Martina Lanza Annalisa Campanella Ciro L. Pierri Marcella Canton Alessandra Castegna
author_sort	Iolanda Spera
title	The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State
title_short	The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State
title_full	The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State
title_fullStr	The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State
title_full_unstemmed	The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State
title_sort	j2-immortalized murine macrophage cell line displays phenotypical and metabolic features of primary bmdms in their m1 and m2 polarization state
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/aeeb579382e4484f841d3b19c190ea7e
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The J2-Immortalized Murine Macrophage Cell Line Displays Phenotypical and Metabolic Features of Primary BMDMs in Their M1 and M2 Polarization State

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