Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis

Phagocytosis requires rapid actin reorganization and spatially controlled force generation to ingest targets ranging from pathogens to apoptotic cells. How actomyosin activity directs membrane extensions to engulf such diverse targets remains unclear. Here, we combine lattice light-sheet microscopy...

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Autores principales:	Daan Vorselen, Sarah R Barger, Yifan Wang, Wei Cai, Julie A Theriot, Nils C Gauthier, Mira Krendel
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	phagocytosis cytoskeleton actin myosin Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/aef0f4ad09434fbab2364d8bb7e93e64
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spelling	oai:doaj.org-article:aef0f4ad09434fbab2364d8bb7e93e642021-11-24T12:33:37ZPhagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis10.7554/eLife.686272050-084Xe68627https://doaj.org/article/aef0f4ad09434fbab2364d8bb7e93e642021-10-01T00:00:00Zhttps://elifesciences.org/articles/68627https://doaj.org/toc/2050-084XPhagocytosis requires rapid actin reorganization and spatially controlled force generation to ingest targets ranging from pathogens to apoptotic cells. How actomyosin activity directs membrane extensions to engulf such diverse targets remains unclear. Here, we combine lattice light-sheet microscopy (LLSM) with microparticle traction force microscopy (MP-TFM) to quantify actin dynamics and subcellular forces during macrophage phagocytosis. We show that spatially localized forces leading to target constriction are prominent during phagocytosis of antibody-opsonized targets. This constriction is largely driven by Arp2/3-mediated assembly of discrete actin protrusions containing myosin 1e and 1f (‘teeth’) that appear to be interconnected in a ring-like organization. Contractile myosin-II activity contributes to late-stage phagocytic force generation and progression, supporting a specific role in phagocytic cup closure. Observations of partial target eating attempts and sudden target release via a popping mechanism suggest that constriction may be critical for resolving complex in vivo target encounters. Overall, our findings present a phagocytic cup shaping mechanism that is distinct from cytoskeletal remodeling in 2D cell motility and may contribute to mechanosensing and phagocytic plasticity.Daan VorselenSarah R BargerYifan WangWei CaiJulie A TheriotNils C GauthierMira KrendeleLife Sciences Publications LtdarticlephagocytosiscytoskeletonactinmyosinMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	phagocytosis cytoskeleton actin myosin Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	phagocytosis cytoskeleton actin myosin Medicine R Science Q Biology (General) QH301-705.5 Daan Vorselen Sarah R Barger Yifan Wang Wei Cai Julie A Theriot Nils C Gauthier Mira Krendel Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis
description	Phagocytosis requires rapid actin reorganization and spatially controlled force generation to ingest targets ranging from pathogens to apoptotic cells. How actomyosin activity directs membrane extensions to engulf such diverse targets remains unclear. Here, we combine lattice light-sheet microscopy (LLSM) with microparticle traction force microscopy (MP-TFM) to quantify actin dynamics and subcellular forces during macrophage phagocytosis. We show that spatially localized forces leading to target constriction are prominent during phagocytosis of antibody-opsonized targets. This constriction is largely driven by Arp2/3-mediated assembly of discrete actin protrusions containing myosin 1e and 1f (‘teeth’) that appear to be interconnected in a ring-like organization. Contractile myosin-II activity contributes to late-stage phagocytic force generation and progression, supporting a specific role in phagocytic cup closure. Observations of partial target eating attempts and sudden target release via a popping mechanism suggest that constriction may be critical for resolving complex in vivo target encounters. Overall, our findings present a phagocytic cup shaping mechanism that is distinct from cytoskeletal remodeling in 2D cell motility and may contribute to mechanosensing and phagocytic plasticity.
format	article
author	Daan Vorselen Sarah R Barger Yifan Wang Wei Cai Julie A Theriot Nils C Gauthier Mira Krendel
author_facet	Daan Vorselen Sarah R Barger Yifan Wang Wei Cai Julie A Theriot Nils C Gauthier Mira Krendel
author_sort	Daan Vorselen
title	Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis
title_short	Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis
title_full	Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis
title_fullStr	Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis
title_full_unstemmed	Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis
title_sort	phagocytic ‘teeth’ and myosin-ii ‘jaw’ power target constriction during phagocytosis
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/aef0f4ad09434fbab2364d8bb7e93e64
work_keys_str_mv	AT daanvorselen phagocyticteethandmyosiniijawpowertargetconstrictionduringphagocytosis AT sarahrbarger phagocyticteethandmyosiniijawpowertargetconstrictionduringphagocytosis AT yifanwang phagocyticteethandmyosiniijawpowertargetconstrictionduringphagocytosis AT weicai phagocyticteethandmyosiniijawpowertargetconstrictionduringphagocytosis AT julieatheriot phagocyticteethandmyosiniijawpowertargetconstrictionduringphagocytosis AT nilscgauthier phagocyticteethandmyosiniijawpowertargetconstrictionduringphagocytosis AT mirakrendel phagocyticteethandmyosiniijawpowertargetconstrictionduringphagocytosis
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Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis

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