Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic

Abstract The PspC and Hic proteins of Streptococcus pneumoniae are some of the most variable microbial immune evasion proteins identified to date. Due to structural similarities and conserved binding profiles, it was assumed for a long time that these pneumococcal surface proteins represent a protei...

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Autores principales:	Shanshan Du, Cláudia Vilhena, Samantha King, Alfredo Sahagún-Ruiz, Sven Hammerschmidt, Christine Skerka, Peter F. Zipfel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/aef54875738542ec8764c9a9ee762fee
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spelling	oai:doaj.org-article:aef54875738542ec8764c9a9ee762fee2021-12-02T11:50:40ZMolecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic10.1038/s41598-020-79362-32045-2322https://doaj.org/article/aef54875738542ec8764c9a9ee762fee2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79362-3https://doaj.org/toc/2045-2322Abstract The PspC and Hic proteins of Streptococcus pneumoniae are some of the most variable microbial immune evasion proteins identified to date. Due to structural similarities and conserved binding profiles, it was assumed for a long time that these pneumococcal surface proteins represent a protein family comprised of eleven subgroups. Recently, however, the evaluation of more proteins revealed a greater diversity of individual proteins. In contrast to previous assumptions a pattern evaluation of six PspC and five Hic variants, each representing one of the previously defined subgroups, revealed distinct structural and likely functionally regions of the proteins, and identified nine new domains and new domain alternates. Several domains are unique to PspC and Hic variants, while other domains are also present in other virulence factors encoded by pneumococci and other bacterial pathogens. This knowledge improved pattern evaluation at the level of full-length proteins, allowed a sequence comparison at the domain level and identified domains with a modular composition. This novel strategy increased understanding of individual proteins variability and modular domain composition, enabled a structural and functional characterization at the domain level and furthermore revealed substantial structural differences between PspC and Hic proteins. Given the exceptional genomic diversity of the multifunctional PspC and Hic proteins a detailed structural and functional evaluation need to be performed at the strain level. Such knowledge will also be useful for molecular strain typing and characterizing PspC and Hic proteins from new clinical S. pneumoniae strains.Shanshan DuCláudia VilhenaSamantha KingAlfredo Sahagún-RuizSven HammerschmidtChristine SkerkaPeter F. ZipfelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shanshan Du Cláudia Vilhena Samantha King Alfredo Sahagún-Ruiz Sven Hammerschmidt Christine Skerka Peter F. Zipfel Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic
description	Abstract The PspC and Hic proteins of Streptococcus pneumoniae are some of the most variable microbial immune evasion proteins identified to date. Due to structural similarities and conserved binding profiles, it was assumed for a long time that these pneumococcal surface proteins represent a protein family comprised of eleven subgroups. Recently, however, the evaluation of more proteins revealed a greater diversity of individual proteins. In contrast to previous assumptions a pattern evaluation of six PspC and five Hic variants, each representing one of the previously defined subgroups, revealed distinct structural and likely functionally regions of the proteins, and identified nine new domains and new domain alternates. Several domains are unique to PspC and Hic variants, while other domains are also present in other virulence factors encoded by pneumococci and other bacterial pathogens. This knowledge improved pattern evaluation at the level of full-length proteins, allowed a sequence comparison at the domain level and identified domains with a modular composition. This novel strategy increased understanding of individual proteins variability and modular domain composition, enabled a structural and functional characterization at the domain level and furthermore revealed substantial structural differences between PspC and Hic proteins. Given the exceptional genomic diversity of the multifunctional PspC and Hic proteins a detailed structural and functional evaluation need to be performed at the strain level. Such knowledge will also be useful for molecular strain typing and characterizing PspC and Hic proteins from new clinical S. pneumoniae strains.
format	article
author	Shanshan Du Cláudia Vilhena Samantha King Alfredo Sahagún-Ruiz Sven Hammerschmidt Christine Skerka Peter F. Zipfel
author_facet	Shanshan Du Cláudia Vilhena Samantha King Alfredo Sahagún-Ruiz Sven Hammerschmidt Christine Skerka Peter F. Zipfel
author_sort	Shanshan Du
title	Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic
title_short	Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic
title_full	Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic
title_fullStr	Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic
title_full_unstemmed	Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic
title_sort	molecular analyses identifies new domains and structural differences among streptococcus pneumoniae immune evasion proteins pspc and hic
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/aef54875738542ec8764c9a9ee762fee
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Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic

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