Homologous ELISA for detection of prednisolone in human serum
An enzyme-linked immunosorbent assay to measure prednisolone have been developed. An antibody was raised in rabbits using prednisolone-21-hemisuccinate (PSL-21-HS) conjugated to bovine serum albumin. Similarly, PSL-21-HS was conjugated to horseradish peroxidase to prepare enzyme conjugate. The devel...
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Taylor & Francis Group
2018
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oai:doaj.org-article:aef8e9e898674a6bbdf24a1c24e89de92021-11-26T11:19:46ZHomologous ELISA for detection of prednisolone in human serum0954-01051465-344310.1080/09540105.2017.1376184https://doaj.org/article/aef8e9e898674a6bbdf24a1c24e89de92018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1376184https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443An enzyme-linked immunosorbent assay to measure prednisolone have been developed. An antibody was raised in rabbits using prednisolone-21-hemisuccinate (PSL-21-HS) conjugated to bovine serum albumin. Similarly, PSL-21-HS was conjugated to horseradish peroxidase to prepare enzyme conjugate. The developed assay has been validated for sensitivity and effective displacement at 50% of the assay were 0.078 and 2.64 ng/mL, respectively. This assay showed cross-reaction with only 4 steroids – i.e. progesterone 1.76%, 17α-OH progesterone 5.89%, cortisol 7.69% and prednisone 1.13%, out of 55 analogous steroids. The percent recovery of prednisolone from the exogenously spiked human serum pools was in the range of 94.84–100.17%. The intra-assay and inter-assay coefficients of variation ranged from 5.79% to 8.00% and from 3.23% to 8.63%, respectively. The serum prednisolone values obtained by this method correlated well with the commercially available kit and found to be 0.93.Dinesh KumarHarpal SinghT. G. ShrivastavTaylor & Francis Grouparticleprednisoloneimmunogenantibodyhorseradish peroxidaseelisaAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 369-385 (2018) |
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prednisolone immunogen antibody horseradish peroxidase elisa Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 |
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prednisolone immunogen antibody horseradish peroxidase elisa Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Dinesh Kumar Harpal Singh T. G. Shrivastav Homologous ELISA for detection of prednisolone in human serum |
| description |
An enzyme-linked immunosorbent assay to measure prednisolone have been developed. An antibody was raised in rabbits using prednisolone-21-hemisuccinate (PSL-21-HS) conjugated to bovine serum albumin. Similarly, PSL-21-HS was conjugated to horseradish peroxidase to prepare enzyme conjugate. The developed assay has been validated for sensitivity and effective displacement at 50% of the assay were 0.078 and 2.64 ng/mL, respectively. This assay showed cross-reaction with only 4 steroids – i.e. progesterone 1.76%, 17α-OH progesterone 5.89%, cortisol 7.69% and prednisone 1.13%, out of 55 analogous steroids. The percent recovery of prednisolone from the exogenously spiked human serum pools was in the range of 94.84–100.17%. The intra-assay and inter-assay coefficients of variation ranged from 5.79% to 8.00% and from 3.23% to 8.63%, respectively. The serum prednisolone values obtained by this method correlated well with the commercially available kit and found to be 0.93. |
| format |
article |
| author |
Dinesh Kumar Harpal Singh T. G. Shrivastav |
| author_facet |
Dinesh Kumar Harpal Singh T. G. Shrivastav |
| author_sort |
Dinesh Kumar |
| title |
Homologous ELISA for detection of prednisolone in human serum |
| title_short |
Homologous ELISA for detection of prednisolone in human serum |
| title_full |
Homologous ELISA for detection of prednisolone in human serum |
| title_fullStr |
Homologous ELISA for detection of prednisolone in human serum |
| title_full_unstemmed |
Homologous ELISA for detection of prednisolone in human serum |
| title_sort |
homologous elisa for detection of prednisolone in human serum |
| publisher |
Taylor & Francis Group |
| publishDate |
2018 |
| url |
https://doaj.org/article/aef8e9e898674a6bbdf24a1c24e89de9 |
| work_keys_str_mv |
AT dineshkumar homologouselisafordetectionofprednisoloneinhumanserum AT harpalsingh homologouselisafordetectionofprednisoloneinhumanserum AT tgshrivastav homologouselisafordetectionofprednisoloneinhumanserum |
| _version_ |
1718409555079069696 |