Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha

Abstract. Saptadi D, Heliyanto B, Sudarsono. 2020. Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha. Biodiversitas 21: 5072-5076. The transferability of SSR markers can be used to access the genetic diversity of...

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Autores principales:	Darmawan Saptadi, Bambang Heliyanto, Sudarsono Sudarsono
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	cross-species amplification, ssr marker, interspecific crossing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/aef906d3717d4e97b54bfb02c0edaa3f
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spelling	oai:doaj.org-article:aef906d3717d4e97b54bfb02c0edaa3f2021-11-22T00:44:11ZShort Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha1412-033X2085-472210.13057/biodiv/d211111https://doaj.org/article/aef906d3717d4e97b54bfb02c0edaa3f2020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6739https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Saptadi D, Heliyanto B, Sudarsono. 2020. Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha. Biodiversitas 21: 5072-5076. The transferability of SSR markers can be used to access the genetic diversity of related species. There are four close relatives of Jatropha curcas L in Indonesia, which can be utilized as a new diversity source through the interspecific crossing. This research was conducted to determine the ability of cross-species amplification of SSR markers developed from J. curcas to other Jatropha species (J. integerrima, J. multifida, J. gossypifolia, J. podagrica). It also investigated the relationship between these species. Out of 28 primers checked,11 primers showed cross-species amplification in all the species tested. Primer pairs EU099519, EU099528, and EU099525 have no transferability to other species. The overall percentage of polymorphism (PP) among all species tested was 95%, with the mean genetic similarity (GS) was 0.34. Least PP (17.35%) and highest GS (0.60) was found between pairs of J. podagrica and J. multifida. The correlation between the PP with GS was relatively high (0.75). The farthest and closest genetic distance was found between J. curcas/J. gossypifolia and J. podagrica/J. multifida, respectively. Further, selected primers from this study can be utilized in species differentiation, molecular identification of interspecific hybrids, and exploiting the genetic resource.Darmawan SaptadiBambang HeliyantoSudarsono SudarsonoMBI & UNS Soloarticlecross-species amplification, ssr marker, interspecific crossingBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cross-species amplification, ssr marker, interspecific crossing Biology (General) QH301-705.5
spellingShingle	cross-species amplification, ssr marker, interspecific crossing Biology (General) QH301-705.5 Darmawan Saptadi Bambang Heliyanto Sudarsono Sudarsono Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha
description	Abstract. Saptadi D, Heliyanto B, Sudarsono. 2020. Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha. Biodiversitas 21: 5072-5076. The transferability of SSR markers can be used to access the genetic diversity of related species. There are four close relatives of Jatropha curcas L in Indonesia, which can be utilized as a new diversity source through the interspecific crossing. This research was conducted to determine the ability of cross-species amplification of SSR markers developed from J. curcas to other Jatropha species (J. integerrima, J. multifida, J. gossypifolia, J. podagrica). It also investigated the relationship between these species. Out of 28 primers checked,11 primers showed cross-species amplification in all the species tested. Primer pairs EU099519, EU099528, and EU099525 have no transferability to other species. The overall percentage of polymorphism (PP) among all species tested was 95%, with the mean genetic similarity (GS) was 0.34. Least PP (17.35%) and highest GS (0.60) was found between pairs of J. podagrica and J. multifida. The correlation between the PP with GS was relatively high (0.75). The farthest and closest genetic distance was found between J. curcas/J. gossypifolia and J. podagrica/J. multifida, respectively. Further, selected primers from this study can be utilized in species differentiation, molecular identification of interspecific hybrids, and exploiting the genetic resource.
format	article
author	Darmawan Saptadi Bambang Heliyanto Sudarsono Sudarsono
author_facet	Darmawan Saptadi Bambang Heliyanto Sudarsono Sudarsono
author_sort	Darmawan Saptadi
title	Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha
title_short	Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha
title_full	Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha
title_fullStr	Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha
title_full_unstemmed	Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha
title_sort	short communication: cross-species amplification of microsatellite markers developed for jatropha curcas within five species of jatropha
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/aef906d3717d4e97b54bfb02c0edaa3f
work_keys_str_mv	AT darmawansaptadi shortcommunicationcrossspeciesamplificationofmicrosatellitemarkersdevelopedforjatrophacurcaswithinfivespeciesofjatropha AT bambangheliyanto shortcommunicationcrossspeciesamplificationofmicrosatellitemarkersdevelopedforjatrophacurcaswithinfivespeciesofjatropha AT sudarsonosudarsono shortcommunicationcrossspeciesamplificationofmicrosatellitemarkersdevelopedforjatrophacurcaswithinfivespeciesofjatropha
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Short Communication: Cross-species amplification of microsatellite markers developed for Jatropha curcas within five species of Jatropha

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