A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression

Abstract The study of miRNAs and their roles as non-invasive biomarkers has been intensely conducted in cancer diseases over the past decade. Various platforms, ranging from conventional qPCRs to Next Generation Sequencers (NGS), have been widely used to analyze miRNA expression. Here we introduced...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chia-Hsun Hsieh, Wei-Ming Chen, Yi-Shan Hsieh, Ya-Chun Fan, Pok Eric Yang, Shih-Ting Kang, Chun-Ta Liao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af01652706e54c5caca177a1684e19c5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:af01652706e54c5caca177a1684e19c5
record_format	dspace
spelling	oai:doaj.org-article:af01652706e54c5caca177a1684e19c52021-12-02T16:08:25ZA Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression10.1038/s41598-018-29146-72045-2322https://doaj.org/article/af01652706e54c5caca177a1684e19c52018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29146-7https://doaj.org/toc/2045-2322Abstract The study of miRNAs and their roles as non-invasive biomarkers has been intensely conducted in cancer diseases over the past decade. Various platforms, ranging from conventional qPCRs to Next Generation Sequencers (NGS), have been widely used to analyze miRNA expression. Here we introduced a novel platform, PanelChip™ Analysis System, which provides a sensitive solution for the analysis of miRNA levels in blood. After conducting miRQC analysis, the system’s analytical performance compared favorably against similar nanoscale qPCR-based array technologies. Because PanelChip™ requires only a minimal amount of miRNA for analysis, we used it to screen for potential diagnostic biomarkers in the plasma of patients with oral cavity squamous cell carcinoma (OSCC). Combining the platform with a machine learning algorithm, we were able to discover miRNA expression patterns capable of separating healthy subjects from patients with OSCC.Chia-Hsun HsiehWei-Ming ChenYi-Shan HsiehYa-Chun FanPok Eric YangShih-Ting KangChun-Ta LiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chia-Hsun Hsieh Wei-Ming Chen Yi-Shan Hsieh Ya-Chun Fan Pok Eric Yang Shih-Ting Kang Chun-Ta Liao A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression
description	Abstract The study of miRNAs and their roles as non-invasive biomarkers has been intensely conducted in cancer diseases over the past decade. Various platforms, ranging from conventional qPCRs to Next Generation Sequencers (NGS), have been widely used to analyze miRNA expression. Here we introduced a novel platform, PanelChip™ Analysis System, which provides a sensitive solution for the analysis of miRNA levels in blood. After conducting miRQC analysis, the system’s analytical performance compared favorably against similar nanoscale qPCR-based array technologies. Because PanelChip™ requires only a minimal amount of miRNA for analysis, we used it to screen for potential diagnostic biomarkers in the plasma of patients with oral cavity squamous cell carcinoma (OSCC). Combining the platform with a machine learning algorithm, we were able to discover miRNA expression patterns capable of separating healthy subjects from patients with OSCC.
format	article
author	Chia-Hsun Hsieh Wei-Ming Chen Yi-Shan Hsieh Ya-Chun Fan Pok Eric Yang Shih-Ting Kang Chun-Ta Liao
author_facet	Chia-Hsun Hsieh Wei-Ming Chen Yi-Shan Hsieh Ya-Chun Fan Pok Eric Yang Shih-Ting Kang Chun-Ta Liao
author_sort	Chia-Hsun Hsieh
title	A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression
title_short	A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression
title_full	A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression
title_fullStr	A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression
title_full_unstemmed	A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression
title_sort	novel multi-gene detection platform for the analysis of mirna expression
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/af01652706e54c5caca177a1684e19c5
work_keys_str_mv	AT chiahsunhsieh anovelmultigenedetectionplatformfortheanalysisofmirnaexpression AT weimingchen anovelmultigenedetectionplatformfortheanalysisofmirnaexpression AT yishanhsieh anovelmultigenedetectionplatformfortheanalysisofmirnaexpression AT yachunfan anovelmultigenedetectionplatformfortheanalysisofmirnaexpression AT pokericyang anovelmultigenedetectionplatformfortheanalysisofmirnaexpression AT shihtingkang anovelmultigenedetectionplatformfortheanalysisofmirnaexpression AT chuntaliao anovelmultigenedetectionplatformfortheanalysisofmirnaexpression AT chiahsunhsieh novelmultigenedetectionplatformfortheanalysisofmirnaexpression AT weimingchen novelmultigenedetectionplatformfortheanalysisofmirnaexpression AT yishanhsieh novelmultigenedetectionplatformfortheanalysisofmirnaexpression AT yachunfan novelmultigenedetectionplatformfortheanalysisofmirnaexpression AT pokericyang novelmultigenedetectionplatformfortheanalysisofmirnaexpression AT shihtingkang novelmultigenedetectionplatformfortheanalysisofmirnaexpression AT chuntaliao novelmultigenedetectionplatformfortheanalysisofmirnaexpression
_version_	1718384490138566656

A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression

Ejemplares similares