Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease

Abstract Chronic wasting disease (CWD) is an emerging infectious prion disorder that is spreading rapidly in wild populations of cervids in North America. The risk of zoonotic transmission of CWD is as yet unclear but a high priority must be to minimize further spread of the disease. No simple diagn...

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Autores principales:	Jessy A. Slota, Sarah J. Medina, Megan Klassen, Damian Gorski, Christine M. Mesa, Catherine Robertson, Gordon Mitchell, Michael B. Coulthart, Sandra Pritzkow, Claudio Soto, Stephanie A. Booth
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af040d673aa34dee92178211e6bf509e
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spelling	oai:doaj.org-article:af040d673aa34dee92178211e6bf509e2021-12-02T13:35:12ZIdentification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease10.1038/s41598-019-56249-62045-2322https://doaj.org/article/af040d673aa34dee92178211e6bf509e2019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56249-6https://doaj.org/toc/2045-2322Abstract Chronic wasting disease (CWD) is an emerging infectious prion disorder that is spreading rapidly in wild populations of cervids in North America. The risk of zoonotic transmission of CWD is as yet unclear but a high priority must be to minimize further spread of the disease. No simple diagnostic tests are available to detect CWD quickly or in live animals; therefore, easily accessible biomarkers may be useful in identifying infected animals. MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that circulate in blood and are promising biomarkers for several infectious diseases. In this study we used next-generation sequencing to characterize the serum miRNA profiles of 35 naturally infected elk that tested positive for CWD in addition to 35 elk that tested negative for CWD. A total of 21 miRNAs that are highly conserved amongst mammals were altered in abundance in sera, irrespective of hemolysis in the samples. A number of these miRNAs have previously been associated with prion diseases. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the discriminative potential of these miRNAs as biomarkers for the diagnosis of CWD. We also determined that a subgroup of 6 of these miRNAs were consistently altered in abundance in serum from hamsters experimentally infected with scrapie. This suggests that common miRNA candidate biomarkers could be selected for prion diseases in multiple species. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses pointed to a strong correlation for 3 of these miRNAs, miR-148a-3p, miR-186-5p, miR-30e-3p, with prion disease.Jessy A. SlotaSarah J. MedinaMegan KlassenDamian GorskiChristine M. MesaCatherine RobertsonGordon MitchellMichael B. CoulthartSandra PritzkowClaudio SotoStephanie A. BoothNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jessy A. Slota Sarah J. Medina Megan Klassen Damian Gorski Christine M. Mesa Catherine Robertson Gordon Mitchell Michael B. Coulthart Sandra Pritzkow Claudio Soto Stephanie A. Booth Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
description	Abstract Chronic wasting disease (CWD) is an emerging infectious prion disorder that is spreading rapidly in wild populations of cervids in North America. The risk of zoonotic transmission of CWD is as yet unclear but a high priority must be to minimize further spread of the disease. No simple diagnostic tests are available to detect CWD quickly or in live animals; therefore, easily accessible biomarkers may be useful in identifying infected animals. MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that circulate in blood and are promising biomarkers for several infectious diseases. In this study we used next-generation sequencing to characterize the serum miRNA profiles of 35 naturally infected elk that tested positive for CWD in addition to 35 elk that tested negative for CWD. A total of 21 miRNAs that are highly conserved amongst mammals were altered in abundance in sera, irrespective of hemolysis in the samples. A number of these miRNAs have previously been associated with prion diseases. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the discriminative potential of these miRNAs as biomarkers for the diagnosis of CWD. We also determined that a subgroup of 6 of these miRNAs were consistently altered in abundance in serum from hamsters experimentally infected with scrapie. This suggests that common miRNA candidate biomarkers could be selected for prion diseases in multiple species. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses pointed to a strong correlation for 3 of these miRNAs, miR-148a-3p, miR-186-5p, miR-30e-3p, with prion disease.
format	article
author	Jessy A. Slota Sarah J. Medina Megan Klassen Damian Gorski Christine M. Mesa Catherine Robertson Gordon Mitchell Michael B. Coulthart Sandra Pritzkow Claudio Soto Stephanie A. Booth
author_facet	Jessy A. Slota Sarah J. Medina Megan Klassen Damian Gorski Christine M. Mesa Catherine Robertson Gordon Mitchell Michael B. Coulthart Sandra Pritzkow Claudio Soto Stephanie A. Booth
author_sort	Jessy A. Slota
title	Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
title_short	Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
title_full	Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
title_fullStr	Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
title_full_unstemmed	Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
title_sort	identification of circulating microrna signatures as potential biomarkers in the serum of elk infected with chronic wasting disease
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/af040d673aa34dee92178211e6bf509e
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Identification of circulating microRNA signatures as potential biomarkers in the serum of elk infected with chronic wasting disease

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