Evaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and RNA sequencing

Abstract We aimed to investigate the effect of chronic particulate matter (PM) exposure on bleomycin-induced lung fibrosis in a rat model using chest CT, histopathologic evaluation, and RNA-sequencing. A bleomycin solution was intratracheally administrated to 20 male rats. For chronic PM exposure, a...

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Autores principales:	Cherry Kim, Sang Hoon Jeong, Jaeyoung Kim, Ja Young Kang, Yoon Jeong Nam, Ariunaa Togloom, Jaehyung Cha, Ki Yeol Lee, Chang Hyun Lee, Eun-Kee Park, Ju-Han Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af07f7552d7c4c518ef0a1ee9e5ec0cf
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spelling	oai:doaj.org-article:af07f7552d7c4c518ef0a1ee9e5ec0cf2021-11-28T12:15:28ZEvaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and RNA sequencing10.1038/s41598-021-02140-22045-2322https://doaj.org/article/af07f7552d7c4c518ef0a1ee9e5ec0cf2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02140-2https://doaj.org/toc/2045-2322Abstract We aimed to investigate the effect of chronic particulate matter (PM) exposure on bleomycin-induced lung fibrosis in a rat model using chest CT, histopathologic evaluation, and RNA-sequencing. A bleomycin solution was intratracheally administrated to 20 male rats. For chronic PM exposure, after four weeks of bleomycin treatment to induce lung fibrosis, PM suspension (experimental group) or normal saline (control group) was intratracheally administrated for 10 weeks. Chest CT was carried out in all rats, and then both lungs were extracted for histopathologic evaluation. One lobe from three rats in each group underwent RNA sequencing, and one lobe from five rats in each group was evaluated by western blotting. Inflammation and fibrosis scores in both chest CT and pathologic analysis were significantly more aggravated in rats with chronic PM exposure than in the control group. Several genes associated with inflammation and immunity were also upregulated with chronic PM exposure. Our study revealed that chronic PM exposure in a bleomycin-induced lung fibrosis rat model aggravated pulmonary fibrosis and inflammation, proven by chest CT, pathologic analysis, and RNA sequencing.Cherry KimSang Hoon JeongJaeyoung KimJa Young KangYoon Jeong NamAriunaa TogloomJaehyung ChaKi Yeol LeeChang Hyun LeeEun-Kee ParkJu-Han LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cherry Kim Sang Hoon Jeong Jaeyoung Kim Ja Young Kang Yoon Jeong Nam Ariunaa Togloom Jaehyung Cha Ki Yeol Lee Chang Hyun Lee Eun-Kee Park Ju-Han Lee Evaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and RNA sequencing
description	Abstract We aimed to investigate the effect of chronic particulate matter (PM) exposure on bleomycin-induced lung fibrosis in a rat model using chest CT, histopathologic evaluation, and RNA-sequencing. A bleomycin solution was intratracheally administrated to 20 male rats. For chronic PM exposure, after four weeks of bleomycin treatment to induce lung fibrosis, PM suspension (experimental group) or normal saline (control group) was intratracheally administrated for 10 weeks. Chest CT was carried out in all rats, and then both lungs were extracted for histopathologic evaluation. One lobe from three rats in each group underwent RNA sequencing, and one lobe from five rats in each group was evaluated by western blotting. Inflammation and fibrosis scores in both chest CT and pathologic analysis were significantly more aggravated in rats with chronic PM exposure than in the control group. Several genes associated with inflammation and immunity were also upregulated with chronic PM exposure. Our study revealed that chronic PM exposure in a bleomycin-induced lung fibrosis rat model aggravated pulmonary fibrosis and inflammation, proven by chest CT, pathologic analysis, and RNA sequencing.
format	article
author	Cherry Kim Sang Hoon Jeong Jaeyoung Kim Ja Young Kang Yoon Jeong Nam Ariunaa Togloom Jaehyung Cha Ki Yeol Lee Chang Hyun Lee Eun-Kee Park Ju-Han Lee
author_facet	Cherry Kim Sang Hoon Jeong Jaeyoung Kim Ja Young Kang Yoon Jeong Nam Ariunaa Togloom Jaehyung Cha Ki Yeol Lee Chang Hyun Lee Eun-Kee Park Ju-Han Lee
author_sort	Cherry Kim
title	Evaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and RNA sequencing
title_short	Evaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and RNA sequencing
title_full	Evaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and RNA sequencing
title_fullStr	Evaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and RNA sequencing
title_full_unstemmed	Evaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and RNA sequencing
title_sort	evaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and rna sequencing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/af07f7552d7c4c518ef0a1ee9e5ec0cf
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Evaluation of the effect of filtered ultrafine particulate matter on bleomycin-induced lung fibrosis in a rat model using computed tomography, histopathologic analysis, and RNA sequencing

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