Diagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors

Abstract We previously identified insulin-like growth factor-II messenger ribonucleic acid-binding protein 3 (IMP3) as a valuable marker to distinguish malignant from benign lesions in pancreatic solid masses. The aim of this prospective study was to evaluate the usefulness of IMP3 and p53 immunohis...

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Autores principales:	Rintaro Mikata, Shin Yasui, Takashi Kishimoto, Yusuke Kouchi, Ayako Shingyoji, Junichi Senoo, Koji Takahashi, Hiroki Nagashima, Yuko Kusakabe, Hiroshi Ohyama, Izumi Ohno, Harutoshi Sugiyama, Tetsuhiro Chiba, Jun Kato, Naoya Kato
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af085d28cefa423da8961acfc1eec724
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spelling	oai:doaj.org-article:af085d28cefa423da8961acfc1eec7242021-12-02T19:02:30ZDiagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors10.1038/s41598-021-96492-42045-2322https://doaj.org/article/af085d28cefa423da8961acfc1eec7242021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96492-4https://doaj.org/toc/2045-2322Abstract We previously identified insulin-like growth factor-II messenger ribonucleic acid-binding protein 3 (IMP3) as a valuable marker to distinguish malignant from benign lesions in pancreatic solid masses. The aim of this prospective study was to evaluate the usefulness of IMP3 and p53 immunohistochemical staining in endoscopic ultrasound-guided fine-needle aspiration (EUS–FNA) samples for pancreatic solid masses. The study recruited 90 consecutive patients with pancreatic masses, including 62 pancreatic ductal adenocarcinomas (PDACs), 11 benign tumors, and 17 other tumors, who underwent EUS–FNA, and conducted IMP3 and p53 immunohistochemical staining. The main outcome measurement was improved diagnostic utility using IMP3 and p53 immunohistochemical staining. IMP3 and p53 expressions were detected in 60.8% and 49.4% of malignant lesions, 69.4% and 58.1% of PDACs, and 0% of benign lesions, respectively. In PDAC and benign tumors, the use of IMP3 and/or p53 immunostaining increased the sensitivity of cytohistological analysis from 88.7 to 93.5%, although the difference was not statistically significant. The sensitivity of histological analysis combined with that of IMP3 staining was 91.9%, which was significantly greater than that of histology alone (80.6%). The use of IMP3 and p53 immunohistochemical staining did not significantly improve the sensitivity of cytohistological analysis; however, IMP3 staining may be helpful for the histological analysis of malignant pancreatic tumors.Rintaro MikataShin YasuiTakashi KishimotoYusuke KouchiAyako ShingyojiJunichi SenooKoji TakahashiHiroki NagashimaYuko KusakabeHiroshi OhyamaIzumi OhnoHarutoshi SugiyamaTetsuhiro ChibaJun KatoNaoya KatoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rintaro Mikata Shin Yasui Takashi Kishimoto Yusuke Kouchi Ayako Shingyoji Junichi Senoo Koji Takahashi Hiroki Nagashima Yuko Kusakabe Hiroshi Ohyama Izumi Ohno Harutoshi Sugiyama Tetsuhiro Chiba Jun Kato Naoya Kato Diagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors
description	Abstract We previously identified insulin-like growth factor-II messenger ribonucleic acid-binding protein 3 (IMP3) as a valuable marker to distinguish malignant from benign lesions in pancreatic solid masses. The aim of this prospective study was to evaluate the usefulness of IMP3 and p53 immunohistochemical staining in endoscopic ultrasound-guided fine-needle aspiration (EUS–FNA) samples for pancreatic solid masses. The study recruited 90 consecutive patients with pancreatic masses, including 62 pancreatic ductal adenocarcinomas (PDACs), 11 benign tumors, and 17 other tumors, who underwent EUS–FNA, and conducted IMP3 and p53 immunohistochemical staining. The main outcome measurement was improved diagnostic utility using IMP3 and p53 immunohistochemical staining. IMP3 and p53 expressions were detected in 60.8% and 49.4% of malignant lesions, 69.4% and 58.1% of PDACs, and 0% of benign lesions, respectively. In PDAC and benign tumors, the use of IMP3 and/or p53 immunostaining increased the sensitivity of cytohistological analysis from 88.7 to 93.5%, although the difference was not statistically significant. The sensitivity of histological analysis combined with that of IMP3 staining was 91.9%, which was significantly greater than that of histology alone (80.6%). The use of IMP3 and p53 immunohistochemical staining did not significantly improve the sensitivity of cytohistological analysis; however, IMP3 staining may be helpful for the histological analysis of malignant pancreatic tumors.
format	article
author	Rintaro Mikata Shin Yasui Takashi Kishimoto Yusuke Kouchi Ayako Shingyoji Junichi Senoo Koji Takahashi Hiroki Nagashima Yuko Kusakabe Hiroshi Ohyama Izumi Ohno Harutoshi Sugiyama Tetsuhiro Chiba Jun Kato Naoya Kato
author_facet	Rintaro Mikata Shin Yasui Takashi Kishimoto Yusuke Kouchi Ayako Shingyoji Junichi Senoo Koji Takahashi Hiroki Nagashima Yuko Kusakabe Hiroshi Ohyama Izumi Ohno Harutoshi Sugiyama Tetsuhiro Chiba Jun Kato Naoya Kato
author_sort	Rintaro Mikata
title	Diagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors
title_short	Diagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors
title_full	Diagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors
title_fullStr	Diagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors
title_full_unstemmed	Diagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors
title_sort	diagnostic value of imp3 and p53 immunohistochemical staining in eus-guided fine-needle aspiration for solid pancreatic tumors
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/af085d28cefa423da8961acfc1eec724
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Diagnostic value of IMP3 and p53 immunohistochemical staining in EUS-guided fine-needle aspiration for solid pancreatic tumors

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