Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections

Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A virus (swIAV) are major pathogens of the porcine respiratory disease complex, but little is known on their interaction in super-infected pigs. In this study, we investigated clinical, virological and immunological outc...

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Autores principales:	Juliette Bougon, Céline Deblanc, Patricia Renson, Stéphane Quéguiner, Stéphane Gorin, Sophie Mahé, Mireille Le Dimna, Nicolas Barbier, Frédéric Paboeuf, Gaëlle Simon, Olivier Bourry
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	swine influenza A virus Betaarterivirus suid 1 porcine respiratory disease complex virus-virus interaction viral interference innate and adaptive immune responses Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/af0bb4a50bb74b5f8d5a94111f83b1f6
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spelling	oai:doaj.org-article:af0bb4a50bb74b5f8d5a94111f83b1f62021-11-25T19:13:00ZSuccessive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections10.3390/v131121691999-4915https://doaj.org/article/af0bb4a50bb74b5f8d5a94111f83b1f62021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2169https://doaj.org/toc/1999-4915Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A virus (swIAV) are major pathogens of the porcine respiratory disease complex, but little is known on their interaction in super-infected pigs. In this study, we investigated clinical, virological and immunological outcomes of successive infections with PRRSV-1 and H1N2 swIAV. Twenty-four specific pathogen-free piglets were distributed into four groups and inoculated either with PRRSV at study day (SD) 0, or with swIAV at SD8, or with PRRSV and swIAV one week apart at SD0 and SD8, respectively, or mock-inoculated. In PRRSV/swIAV group, the clinical signs usually observed after swIAV infection were attenuated while higher levels of anti-swIAV antibodies were measured in lungs. Concurrently, PRRSV multiplication in lungs was significantly affected by swIAV infection, whereas the cell-mediated immune response specific to PRRSV was detected earlier in blood, as compared to PRRSV group. Moreover, levels of interferon (IFN)-α measured from SD9 in the blood of super-infected pigs were lower than those measured in the swIAV group, but higher than in the PRRSV group at the same time. Correlation analyses suggested an important role of IFN-α in the two-way interference highlighted between both viral infections.Juliette BougonCéline DeblancPatricia RensonStéphane QuéguinerStéphane GorinSophie MahéMireille Le DimnaNicolas BarbierFrédéric PaboeufGaëlle SimonOlivier BourryMDPI AGarticleswine influenza A virusBetaarterivirus suid 1porcine respiratory disease complexvirus-virus interactionviral interferenceinnate and adaptive immune responsesMicrobiologyQR1-502ENViruses, Vol 13, Iss 2169, p 2169 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	swine influenza A virus Betaarterivirus suid 1 porcine respiratory disease complex virus-virus interaction viral interference innate and adaptive immune responses Microbiology QR1-502
spellingShingle	swine influenza A virus Betaarterivirus suid 1 porcine respiratory disease complex virus-virus interaction viral interference innate and adaptive immune responses Microbiology QR1-502 Juliette Bougon Céline Deblanc Patricia Renson Stéphane Quéguiner Stéphane Gorin Sophie Mahé Mireille Le Dimna Nicolas Barbier Frédéric Paboeuf Gaëlle Simon Olivier Bourry Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
description	Porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A virus (swIAV) are major pathogens of the porcine respiratory disease complex, but little is known on their interaction in super-infected pigs. In this study, we investigated clinical, virological and immunological outcomes of successive infections with PRRSV-1 and H1N2 swIAV. Twenty-four specific pathogen-free piglets were distributed into four groups and inoculated either with PRRSV at study day (SD) 0, or with swIAV at SD8, or with PRRSV and swIAV one week apart at SD0 and SD8, respectively, or mock-inoculated. In PRRSV/swIAV group, the clinical signs usually observed after swIAV infection were attenuated while higher levels of anti-swIAV antibodies were measured in lungs. Concurrently, PRRSV multiplication in lungs was significantly affected by swIAV infection, whereas the cell-mediated immune response specific to PRRSV was detected earlier in blood, as compared to PRRSV group. Moreover, levels of interferon (IFN)-α measured from SD9 in the blood of super-infected pigs were lower than those measured in the swIAV group, but higher than in the PRRSV group at the same time. Correlation analyses suggested an important role of IFN-α in the two-way interference highlighted between both viral infections.
format	article
author	Juliette Bougon Céline Deblanc Patricia Renson Stéphane Quéguiner Stéphane Gorin Sophie Mahé Mireille Le Dimna Nicolas Barbier Frédéric Paboeuf Gaëlle Simon Olivier Bourry
author_facet	Juliette Bougon Céline Deblanc Patricia Renson Stéphane Quéguiner Stéphane Gorin Sophie Mahé Mireille Le Dimna Nicolas Barbier Frédéric Paboeuf Gaëlle Simon Olivier Bourry
author_sort	Juliette Bougon
title	Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_short	Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_full	Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_fullStr	Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_full_unstemmed	Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections
title_sort	successive inoculations of pigs with porcine reproductive and respiratory syndrome virus 1 (prrsv-1) and swine h1n2 influenza virus suggest a mutual interference between the two viral infections
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/af0bb4a50bb74b5f8d5a94111f83b1f6
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Successive Inoculations of Pigs with Porcine Reproductive and Respiratory Syndrome Virus 1 (PRRSV-1) and Swine H1N2 Influenza Virus Suggest a Mutual Interference between the Two Viral Infections

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