Simultaneous Real-Time Detection of Pregnancy-Associated Plasma Protein-a and -A2 Using a Graphene Oxide-Based Surface Plasmon Resonance Biosensor

Shi-Yuan Fan,1 Nan-Fu Chiu,2 Chie-Pein Chen,1,3 Chia-Chen Chang,4 Chen-Yu Chen1,3 1Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan; 2Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei, Taiwan; 3Department of Medicine, Mack...

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Autores principales:	Fan SY, Chiu NF, Chen CP, Chang CC, Chen CY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	pregnancy-associated plasma protein-a pregnancy-associated plasma protein-a2 graphene oxide surface plasmon resonance biosensor Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/af0f661402514852b26f3fef2d1f5ce3
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spelling	oai:doaj.org-article:af0f661402514852b26f3fef2d1f5ce32021-12-02T03:01:27ZSimultaneous Real-Time Detection of Pregnancy-Associated Plasma Protein-a and -A2 Using a Graphene Oxide-Based Surface Plasmon Resonance Biosensor1178-2013https://doaj.org/article/af0f661402514852b26f3fef2d1f5ce32020-03-01T00:00:00Zhttps://www.dovepress.com/simultaneous-real-time-detection-of-pregnancy-associated-plasma-protei-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shi-Yuan Fan,1 Nan-Fu Chiu,2 Chie-Pein Chen,1,3 Chia-Chen Chang,4 Chen-Yu Chen1,3 1Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan; 2Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei, Taiwan; 3Department of Medicine, Mackay Medical College, New Taipei City, Taiwan; 4Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan City, TaiwanCorrespondence: Chen-Yu ChenDepartment of Medicine, Mackay Medical College, No. 46, Section 3, Zhongzheng Road, Sanzhi District, New Taipei City 252, TaiwanTel +886 2 2636 0303Email f122481@mmh.org.twBackground: Pregnancy-associated plasma protein-A and -A2 (PAPP-A and -A2) are principally expressed in placental trophoblasts and play a critical role in the regulation of fetal and placental growth. PAPP-A2 shares 45% amino acid similarity with PAPP-A. This study aimed to investigate the efficacy of real-time detection of PAPP-A and PAPP-A2 using a novel surface plasmon resonance (SPR) biosensor based on graphene oxide (GO).Methods: Traditional SPR and GO-based SPR chips were fabricated to measure PAPP-A and PAPP-A2 concentrations. We compared SPR response curves of PAPP-A and PAPP-A2 between traditional SPR and GO-SPR biosensors. We also performed interference tests and specificity analyses among PAPP-A, PAPP-A2, and mixed interference proteins.Results: The time to detect PAPP-A and PAPP-A2 was about 150 seconds with both traditional SPR and GO-SPR biosensors. Approximately double SPR angle shifts were noted with the GO-SPR biosensor compared to the traditional SPR biosensor at a PAPP-A and PAPP-A2 concentration of 5 μg/mL. The limit of detection of the GO-SPR biosensor was as low as 0.5 ng/mL for both PAPP-A and PAPP-A2. Interference testing revealed that almost all of the protein bonded on the GO-SPR biosensor with anti-PAPP-A from the mixture of proteins was PAPP-A, and that almost no other proteins were captured except for PAPP-A2. However, the SPR signal of PAPP-A2 (5.75 mdeg) was much smaller than that of PAPP-A (13.76 mdeg). Similar results were noted with anti-PAPP-A2, where almost all of the protein bonded on the GO-SPR biosensor was PAPP-A2. The SPR signal of PAPP-A (5.17 mdeg) was much smaller than that of PAPP-A2 (13.94 mdeg).Conclusion: The GO-SPR biosensor could distinguish PAPP-A and PAPP-A2 from various mixed interference proteins with high sensitivity and specificity. It could potentially be used to measure PAPP-A and PAPP-A2 in clinical blood samples during pregnancy.Keywords: pregnancy-associated plasma protein-A, pregnancy-associated plasma protein-A2, graphene oxide, surface plasmon resonance, biosensorFan SYChiu NFChen CPChang CCChen CYDove Medical Pressarticlepregnancy-associated plasma protein-apregnancy-associated plasma protein-a2graphene oxidesurface plasmon resonancebiosensorMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 2085-2094 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	pregnancy-associated plasma protein-a pregnancy-associated plasma protein-a2 graphene oxide surface plasmon resonance biosensor Medicine (General) R5-920
spellingShingle	pregnancy-associated plasma protein-a pregnancy-associated plasma protein-a2 graphene oxide surface plasmon resonance biosensor Medicine (General) R5-920 Fan SY Chiu NF Chen CP Chang CC Chen CY Simultaneous Real-Time Detection of Pregnancy-Associated Plasma Protein-a and -A2 Using a Graphene Oxide-Based Surface Plasmon Resonance Biosensor
description	Shi-Yuan Fan,1 Nan-Fu Chiu,2 Chie-Pein Chen,1,3 Chia-Chen Chang,4 Chen-Yu Chen1,3 1Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan; 2Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei, Taiwan; 3Department of Medicine, Mackay Medical College, New Taipei City, Taiwan; 4Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan City, TaiwanCorrespondence: Chen-Yu ChenDepartment of Medicine, Mackay Medical College, No. 46, Section 3, Zhongzheng Road, Sanzhi District, New Taipei City 252, TaiwanTel +886 2 2636 0303Email f122481@mmh.org.twBackground: Pregnancy-associated plasma protein-A and -A2 (PAPP-A and -A2) are principally expressed in placental trophoblasts and play a critical role in the regulation of fetal and placental growth. PAPP-A2 shares 45% amino acid similarity with PAPP-A. This study aimed to investigate the efficacy of real-time detection of PAPP-A and PAPP-A2 using a novel surface plasmon resonance (SPR) biosensor based on graphene oxide (GO).Methods: Traditional SPR and GO-based SPR chips were fabricated to measure PAPP-A and PAPP-A2 concentrations. We compared SPR response curves of PAPP-A and PAPP-A2 between traditional SPR and GO-SPR biosensors. We also performed interference tests and specificity analyses among PAPP-A, PAPP-A2, and mixed interference proteins.Results: The time to detect PAPP-A and PAPP-A2 was about 150 seconds with both traditional SPR and GO-SPR biosensors. Approximately double SPR angle shifts were noted with the GO-SPR biosensor compared to the traditional SPR biosensor at a PAPP-A and PAPP-A2 concentration of 5 μg/mL. The limit of detection of the GO-SPR biosensor was as low as 0.5 ng/mL for both PAPP-A and PAPP-A2. Interference testing revealed that almost all of the protein bonded on the GO-SPR biosensor with anti-PAPP-A from the mixture of proteins was PAPP-A, and that almost no other proteins were captured except for PAPP-A2. However, the SPR signal of PAPP-A2 (5.75 mdeg) was much smaller than that of PAPP-A (13.76 mdeg). Similar results were noted with anti-PAPP-A2, where almost all of the protein bonded on the GO-SPR biosensor was PAPP-A2. The SPR signal of PAPP-A (5.17 mdeg) was much smaller than that of PAPP-A2 (13.94 mdeg).Conclusion: The GO-SPR biosensor could distinguish PAPP-A and PAPP-A2 from various mixed interference proteins with high sensitivity and specificity. It could potentially be used to measure PAPP-A and PAPP-A2 in clinical blood samples during pregnancy.Keywords: pregnancy-associated plasma protein-A, pregnancy-associated plasma protein-A2, graphene oxide, surface plasmon resonance, biosensor
format	article
author	Fan SY Chiu NF Chen CP Chang CC Chen CY
author_facet	Fan SY Chiu NF Chen CP Chang CC Chen CY
author_sort	Fan SY
title	Simultaneous Real-Time Detection of Pregnancy-Associated Plasma Protein-a and -A2 Using a Graphene Oxide-Based Surface Plasmon Resonance Biosensor
title_short	Simultaneous Real-Time Detection of Pregnancy-Associated Plasma Protein-a and -A2 Using a Graphene Oxide-Based Surface Plasmon Resonance Biosensor
title_full	Simultaneous Real-Time Detection of Pregnancy-Associated Plasma Protein-a and -A2 Using a Graphene Oxide-Based Surface Plasmon Resonance Biosensor
title_fullStr	Simultaneous Real-Time Detection of Pregnancy-Associated Plasma Protein-a and -A2 Using a Graphene Oxide-Based Surface Plasmon Resonance Biosensor
title_full_unstemmed	Simultaneous Real-Time Detection of Pregnancy-Associated Plasma Protein-a and -A2 Using a Graphene Oxide-Based Surface Plasmon Resonance Biosensor
title_sort	simultaneous real-time detection of pregnancy-associated plasma protein-a and -a2 using a graphene oxide-based surface plasmon resonance biosensor
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/af0f661402514852b26f3fef2d1f5ce3
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_version_	1718402003778928640

Simultaneous Real-Time Detection of Pregnancy-Associated Plasma Protein-a and -A2 Using a Graphene Oxide-Based Surface Plasmon Resonance Biosensor

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