Inhibition of endoplasmic reticulum stress improves mouse embryo development.

X-box binding protein-1 (XBP-1) is an important regulator of a subset of genes during endoplasmic reticulum (ER) stress. In the current study, we analyzed endogenous XBP-1 expression and localization, with a view to determining the effects of ER stress on the developmental competency of preimplantat...

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Autores principales:	Jin Yu Zhang, Yun Fei Diao, Hong Rye Kim, Dong Il Jin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af1573464ad840d9837ebb156f25a26b
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spelling	oai:doaj.org-article:af1573464ad840d9837ebb156f25a26b2021-11-18T07:12:34ZInhibition of endoplasmic reticulum stress improves mouse embryo development.1932-620310.1371/journal.pone.0040433https://doaj.org/article/af1573464ad840d9837ebb156f25a26b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22808162/?tool=EBIhttps://doaj.org/toc/1932-6203X-box binding protein-1 (XBP-1) is an important regulator of a subset of genes during endoplasmic reticulum (ER) stress. In the current study, we analyzed endogenous XBP-1 expression and localization, with a view to determining the effects of ER stress on the developmental competency of preimplantation embryos in mice. Fluorescence staining revealed that functional XBP-1 is localized on mature oocyte spindles and abundant in the nucleus at the germinal vesicle (GV) stage. However, in preimplantation embryos, XBP-1 was solely detected in the cytoplasm at the one-cell stage. The density of XBP-1 was higher in the nucleus than the cytoplasm at the two-cell, four-cell, eight-cell, morula, and blastocyst stages. Furthermore, RT-PCR analysis confirmed active XBP-1 mRNA splicing at all preimplantation embryo stages, except the one-cell stage. Tunicamycin (TM), an ER stress inducer used as a positive control, promoted an increase in the density of nuclear XBP-1 at the one-cell and two-cell stages. Similarly, culture medium supplemented with 25 mM sorbitol displayed a remarkable increase active XBP-1 expression in the nuclei of 1-cell and 2-cell embryos. Conversely, high concentrations of TM or sorbitol led to reduced nuclear XBP-1 density and significant ER stress-induced apoptosis. Tauroursodeoxycholic acid (TUDCA), a known inhibitor of ER stress, improved the rate of two-cell embryo development to blastocysts by attenuating the expression of active XBP-1 protein in the nucleus at the two-cell stage. Our data collectively suggest that endogenous XBP-1 plays a role in normal preimplantation embryonic development. Moreover, XBP-1 splicing is activated to generate a functional form in mouse preimplantation embryos during culture stress. TUDCA inhibits hyperosmolar-induced ER stress as well as ER stress-induced apoptosis during mouse preimplantation embryo development.Jin Yu ZhangYun Fei DiaoHong Rye KimDong Il JinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40433 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jin Yu Zhang Yun Fei Diao Hong Rye Kim Dong Il Jin Inhibition of endoplasmic reticulum stress improves mouse embryo development.
description	X-box binding protein-1 (XBP-1) is an important regulator of a subset of genes during endoplasmic reticulum (ER) stress. In the current study, we analyzed endogenous XBP-1 expression and localization, with a view to determining the effects of ER stress on the developmental competency of preimplantation embryos in mice. Fluorescence staining revealed that functional XBP-1 is localized on mature oocyte spindles and abundant in the nucleus at the germinal vesicle (GV) stage. However, in preimplantation embryos, XBP-1 was solely detected in the cytoplasm at the one-cell stage. The density of XBP-1 was higher in the nucleus than the cytoplasm at the two-cell, four-cell, eight-cell, morula, and blastocyst stages. Furthermore, RT-PCR analysis confirmed active XBP-1 mRNA splicing at all preimplantation embryo stages, except the one-cell stage. Tunicamycin (TM), an ER stress inducer used as a positive control, promoted an increase in the density of nuclear XBP-1 at the one-cell and two-cell stages. Similarly, culture medium supplemented with 25 mM sorbitol displayed a remarkable increase active XBP-1 expression in the nuclei of 1-cell and 2-cell embryos. Conversely, high concentrations of TM or sorbitol led to reduced nuclear XBP-1 density and significant ER stress-induced apoptosis. Tauroursodeoxycholic acid (TUDCA), a known inhibitor of ER stress, improved the rate of two-cell embryo development to blastocysts by attenuating the expression of active XBP-1 protein in the nucleus at the two-cell stage. Our data collectively suggest that endogenous XBP-1 plays a role in normal preimplantation embryonic development. Moreover, XBP-1 splicing is activated to generate a functional form in mouse preimplantation embryos during culture stress. TUDCA inhibits hyperosmolar-induced ER stress as well as ER stress-induced apoptosis during mouse preimplantation embryo development.
format	article
author	Jin Yu Zhang Yun Fei Diao Hong Rye Kim Dong Il Jin
author_facet	Jin Yu Zhang Yun Fei Diao Hong Rye Kim Dong Il Jin
author_sort	Jin Yu Zhang
title	Inhibition of endoplasmic reticulum stress improves mouse embryo development.
title_short	Inhibition of endoplasmic reticulum stress improves mouse embryo development.
title_full	Inhibition of endoplasmic reticulum stress improves mouse embryo development.
title_fullStr	Inhibition of endoplasmic reticulum stress improves mouse embryo development.
title_full_unstemmed	Inhibition of endoplasmic reticulum stress improves mouse embryo development.
title_sort	inhibition of endoplasmic reticulum stress improves mouse embryo development.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/af1573464ad840d9837ebb156f25a26b
work_keys_str_mv	AT jinyuzhang inhibitionofendoplasmicreticulumstressimprovesmouseembryodevelopment AT yunfeidiao inhibitionofendoplasmicreticulumstressimprovesmouseembryodevelopment AT hongryekim inhibitionofendoplasmicreticulumstressimprovesmouseembryodevelopment AT dongiljin inhibitionofendoplasmicreticulumstressimprovesmouseembryodevelopment
_version_	1718423809108738048

Inhibition of endoplasmic reticulum stress improves mouse embryo development.

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