Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila.

Heat-shock genes have a well-studied control mechanism for their expression that is mediated through cis-regulatory motifs known as heat-shock elements (HSEs). The evolution of important features of this control mechanism has not been investigated in detail, however. Here we exploit the genome seque...

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Autores principales:	Sibo Tian, Robert A Haney, Martin E Feder
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af1ef7add9084173b41f4251bb438bab
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spelling	oai:doaj.org-article:af1ef7add9084173b41f4251bb438bab2021-12-02T20:21:37ZPhylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila.1932-620310.1371/journal.pone.0010669https://doaj.org/article/af1ef7add9084173b41f4251bb438bab2010-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20498853/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Heat-shock genes have a well-studied control mechanism for their expression that is mediated through cis-regulatory motifs known as heat-shock elements (HSEs). The evolution of important features of this control mechanism has not been investigated in detail, however. Here we exploit the genome sequencing of multiple Drosophila species, combined with a wealth of available information on the structure and function of HSEs in D. melanogaster, to undertake this investigation. We find that in single-copy heat shock genes, entire HSEs have evolved or disappeared 14 times, and the phylogenetic approach bounds the timing and direction of these evolutionary events in relation to speciation. In contrast, in the multi-copy gene Hsp70, the number of HSEs is nearly constant across species. HSEs evolve in size, position, and sequence within heat-shock promoters. In turn, functional significance of certain features is implicated by preservation despite this evolutionary change; these features include tail-to-tail arrangements of HSEs, gapped HSEs, and the presence or absence of entire HSEs. The variation among Drosophila species indicates that the cis-regulatory encoding of responsiveness to heat and other stresses is diverse. The broad dimensions of variation uncovered are particularly important as they suggest a substantial challenge for functional studies.Sibo TianRobert A HaneyMartin E FederPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 5, p e10669 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sibo Tian Robert A Haney Martin E Feder Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila.
description	Heat-shock genes have a well-studied control mechanism for their expression that is mediated through cis-regulatory motifs known as heat-shock elements (HSEs). The evolution of important features of this control mechanism has not been investigated in detail, however. Here we exploit the genome sequencing of multiple Drosophila species, combined with a wealth of available information on the structure and function of HSEs in D. melanogaster, to undertake this investigation. We find that in single-copy heat shock genes, entire HSEs have evolved or disappeared 14 times, and the phylogenetic approach bounds the timing and direction of these evolutionary events in relation to speciation. In contrast, in the multi-copy gene Hsp70, the number of HSEs is nearly constant across species. HSEs evolve in size, position, and sequence within heat-shock promoters. In turn, functional significance of certain features is implicated by preservation despite this evolutionary change; these features include tail-to-tail arrangements of HSEs, gapped HSEs, and the presence or absence of entire HSEs. The variation among Drosophila species indicates that the cis-regulatory encoding of responsiveness to heat and other stresses is diverse. The broad dimensions of variation uncovered are particularly important as they suggest a substantial challenge for functional studies.
format	article
author	Sibo Tian Robert A Haney Martin E Feder
author_facet	Sibo Tian Robert A Haney Martin E Feder
author_sort	Sibo Tian
title	Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila.
title_short	Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila.
title_full	Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila.
title_fullStr	Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila.
title_full_unstemmed	Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila.
title_sort	phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/af1ef7add9084173b41f4251bb438bab
work_keys_str_mv	AT sibotian phylogenydisambiguatestheevolutionofheatshockcisregulatoryelementsindrosophila AT robertahaney phylogenydisambiguatestheevolutionofheatshockcisregulatoryelementsindrosophila AT martinefeder phylogenydisambiguatestheevolutionofheatshockcisregulatoryelementsindrosophila
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Phylogeny disambiguates the evolution of heat-shock cis-regulatory elements in Drosophila.

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