Short-term variability of the human serum metabolome depending on nutritional and metabolic health status

Abstract The intra-individual variability of the human serum metabolome over a period of 4 weeks and its dependence on metabolic health and nutritional status was investigated in a single-center study under tightly controlled conditions in healthy controls, pre-diabetic individuals and patients with...

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Autores principales:	Inoncent Agueusop, Petra B. Musholt, Beate Klaus, Kendra Hightower, Aimo Kannt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af2daeff5b9c45e18f466140866829f2
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spelling	oai:doaj.org-article:af2daeff5b9c45e18f466140866829f22021-12-02T18:51:27ZShort-term variability of the human serum metabolome depending on nutritional and metabolic health status10.1038/s41598-020-72914-72045-2322https://doaj.org/article/af2daeff5b9c45e18f466140866829f22020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-72914-7https://doaj.org/toc/2045-2322Abstract The intra-individual variability of the human serum metabolome over a period of 4 weeks and its dependence on metabolic health and nutritional status was investigated in a single-center study under tightly controlled conditions in healthy controls, pre-diabetic individuals and patients with type-2 diabetes mellitus (T2DM, n = 10 each). Untargeted metabolomics in serum samples taken at three different days after overnight fasts and following intake of a standardized mixed meal showed that the human serum metabolome is remarkably stable: The median intra-class correlation coefficient (ICC) across all metabolites and all study participants was determined as 0.65. ICCs were similar for the three different health groups, before and after meal intake, and for different metabolic pathways. Only 147 out of 1438 metabolites (10%) had an ICC below 0.4 indicating poor stability over time. In addition, we confirmed previously identified metabolic signatures differentiating healthy, pre-diabetic and diabetic individuals. To our knowledge, this is the most comprehensive study investigating the temporal variability of the human serum metabolome under such tightly controlled conditions.Inoncent AgueusopPetra B. MusholtBeate KlausKendra HightowerAimo KanntNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Inoncent Agueusop Petra B. Musholt Beate Klaus Kendra Hightower Aimo Kannt Short-term variability of the human serum metabolome depending on nutritional and metabolic health status
description	Abstract The intra-individual variability of the human serum metabolome over a period of 4 weeks and its dependence on metabolic health and nutritional status was investigated in a single-center study under tightly controlled conditions in healthy controls, pre-diabetic individuals and patients with type-2 diabetes mellitus (T2DM, n = 10 each). Untargeted metabolomics in serum samples taken at three different days after overnight fasts and following intake of a standardized mixed meal showed that the human serum metabolome is remarkably stable: The median intra-class correlation coefficient (ICC) across all metabolites and all study participants was determined as 0.65. ICCs were similar for the three different health groups, before and after meal intake, and for different metabolic pathways. Only 147 out of 1438 metabolites (10%) had an ICC below 0.4 indicating poor stability over time. In addition, we confirmed previously identified metabolic signatures differentiating healthy, pre-diabetic and diabetic individuals. To our knowledge, this is the most comprehensive study investigating the temporal variability of the human serum metabolome under such tightly controlled conditions.
format	article
author	Inoncent Agueusop Petra B. Musholt Beate Klaus Kendra Hightower Aimo Kannt
author_facet	Inoncent Agueusop Petra B. Musholt Beate Klaus Kendra Hightower Aimo Kannt
author_sort	Inoncent Agueusop
title	Short-term variability of the human serum metabolome depending on nutritional and metabolic health status
title_short	Short-term variability of the human serum metabolome depending on nutritional and metabolic health status
title_full	Short-term variability of the human serum metabolome depending on nutritional and metabolic health status
title_fullStr	Short-term variability of the human serum metabolome depending on nutritional and metabolic health status
title_full_unstemmed	Short-term variability of the human serum metabolome depending on nutritional and metabolic health status
title_sort	short-term variability of the human serum metabolome depending on nutritional and metabolic health status
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/af2daeff5b9c45e18f466140866829f2
work_keys_str_mv	AT inoncentagueusop shorttermvariabilityofthehumanserummetabolomedependingonnutritionalandmetabolichealthstatus AT petrabmusholt shorttermvariabilityofthehumanserummetabolomedependingonnutritionalandmetabolichealthstatus AT beateklaus shorttermvariabilityofthehumanserummetabolomedependingonnutritionalandmetabolichealthstatus AT kendrahightower shorttermvariabilityofthehumanserummetabolomedependingonnutritionalandmetabolichealthstatus AT aimokannt shorttermvariabilityofthehumanserummetabolomedependingonnutritionalandmetabolichealthstatus
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Short-term variability of the human serum metabolome depending on nutritional and metabolic health status

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