Bhalin, an Essential Cytoskeleton-Associated Protein of <i>Trypanosoma brucei</i> Linking <i>Tb</i>BILBO1 of the Flagellar Pocket Collar with the Hook Complex
Background: In most trypanosomes, endo and exocytosis only occur at a unique organelle called the flagellar pocket (FP) and the flagellum exits the cell via the FP. Investigations of essential cytoskeleton-associated structures located at this site have revealed a number of essential proteins. The p...
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2021
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oai:doaj.org-article:af2f51dfb5f1471ba8c85b0ce02618862021-11-25T18:25:14ZBhalin, an Essential Cytoskeleton-Associated Protein of <i>Trypanosoma brucei</i> Linking <i>Tb</i>BILBO1 of the Flagellar Pocket Collar with the Hook Complex10.3390/microorganisms91123342076-2607https://doaj.org/article/af2f51dfb5f1471ba8c85b0ce02618862021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2334https://doaj.org/toc/2076-2607Background: In most trypanosomes, endo and exocytosis only occur at a unique organelle called the flagellar pocket (FP) and the flagellum exits the cell via the FP. Investigations of essential cytoskeleton-associated structures located at this site have revealed a number of essential proteins. The protein <i>Tb</i>BILBO1 is located at the neck of the FP in a structure called the flagellar pocket collar (FPC) and is essential for biogenesis of the FPC and parasite survival. <i>Tb</i>MORN1 is a protein that is present on a closely linked structure called the hook complex (HC) and is located anterior to and overlapping the collar. <i>Tb</i>MORN1 is essential in the bloodstream form of <i>T. brucei.</i> We now describe the location and function of BHALIN, an essential, new FPC-HC protein. Methodology/Principal Findings: Here, we show that a newly characterised protein, BHALIN (<span style="text-decoration: underline;">B</span>ILBO1 <span style="text-decoration: underline;">H</span>ook <span style="text-decoration: underline;">A</span>ssociated <span style="text-decoration: underline;">LIN</span>ker protein), is localised to both the FPC and HC and has a <i>Tb</i>BILBO1 binding domain, which was confirmed in vitro. Knockdown of <i>BHALIN</i> by RNAi in the bloodstream form parasites led to cell death, indicating an essential role in cell viability. Conclusions/Significance: Our results demonstrate the essential role of a newly characterised hook complex protein, BHALIN, that influences flagellar pocket organisation and function in bloodstream form <i>T. brucei</i> parasites.Christine E. Broster ReixCélia FlorimondAnne CayrelAmélie MailhéCorentin Agnero-RigotNicolas LandreinDenis DacheuxKatharina HavlicekMélanie BonhiversBrooke MorriswoodDerrick R. RobinsonMDPI AGarticletrypanosomaflagellar pockethook complexendocytosiscytoskeletonprotozoanBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2334, p 2334 (2021) |
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| collection |
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| topic |
trypanosoma flagellar pocket hook complex endocytosis cytoskeleton protozoan Biology (General) QH301-705.5 |
| spellingShingle |
trypanosoma flagellar pocket hook complex endocytosis cytoskeleton protozoan Biology (General) QH301-705.5 Christine E. Broster Reix Célia Florimond Anne Cayrel Amélie Mailhé Corentin Agnero-Rigot Nicolas Landrein Denis Dacheux Katharina Havlicek Mélanie Bonhivers Brooke Morriswood Derrick R. Robinson Bhalin, an Essential Cytoskeleton-Associated Protein of <i>Trypanosoma brucei</i> Linking <i>Tb</i>BILBO1 of the Flagellar Pocket Collar with the Hook Complex |
| description |
Background: In most trypanosomes, endo and exocytosis only occur at a unique organelle called the flagellar pocket (FP) and the flagellum exits the cell via the FP. Investigations of essential cytoskeleton-associated structures located at this site have revealed a number of essential proteins. The protein <i>Tb</i>BILBO1 is located at the neck of the FP in a structure called the flagellar pocket collar (FPC) and is essential for biogenesis of the FPC and parasite survival. <i>Tb</i>MORN1 is a protein that is present on a closely linked structure called the hook complex (HC) and is located anterior to and overlapping the collar. <i>Tb</i>MORN1 is essential in the bloodstream form of <i>T. brucei.</i> We now describe the location and function of BHALIN, an essential, new FPC-HC protein. Methodology/Principal Findings: Here, we show that a newly characterised protein, BHALIN (<span style="text-decoration: underline;">B</span>ILBO1 <span style="text-decoration: underline;">H</span>ook <span style="text-decoration: underline;">A</span>ssociated <span style="text-decoration: underline;">LIN</span>ker protein), is localised to both the FPC and HC and has a <i>Tb</i>BILBO1 binding domain, which was confirmed in vitro. Knockdown of <i>BHALIN</i> by RNAi in the bloodstream form parasites led to cell death, indicating an essential role in cell viability. Conclusions/Significance: Our results demonstrate the essential role of a newly characterised hook complex protein, BHALIN, that influences flagellar pocket organisation and function in bloodstream form <i>T. brucei</i> parasites. |
| format |
article |
| author |
Christine E. Broster Reix Célia Florimond Anne Cayrel Amélie Mailhé Corentin Agnero-Rigot Nicolas Landrein Denis Dacheux Katharina Havlicek Mélanie Bonhivers Brooke Morriswood Derrick R. Robinson |
| author_facet |
Christine E. Broster Reix Célia Florimond Anne Cayrel Amélie Mailhé Corentin Agnero-Rigot Nicolas Landrein Denis Dacheux Katharina Havlicek Mélanie Bonhivers Brooke Morriswood Derrick R. Robinson |
| author_sort |
Christine E. Broster Reix |
| title |
Bhalin, an Essential Cytoskeleton-Associated Protein of <i>Trypanosoma brucei</i> Linking <i>Tb</i>BILBO1 of the Flagellar Pocket Collar with the Hook Complex |
| title_short |
Bhalin, an Essential Cytoskeleton-Associated Protein of <i>Trypanosoma brucei</i> Linking <i>Tb</i>BILBO1 of the Flagellar Pocket Collar with the Hook Complex |
| title_full |
Bhalin, an Essential Cytoskeleton-Associated Protein of <i>Trypanosoma brucei</i> Linking <i>Tb</i>BILBO1 of the Flagellar Pocket Collar with the Hook Complex |
| title_fullStr |
Bhalin, an Essential Cytoskeleton-Associated Protein of <i>Trypanosoma brucei</i> Linking <i>Tb</i>BILBO1 of the Flagellar Pocket Collar with the Hook Complex |
| title_full_unstemmed |
Bhalin, an Essential Cytoskeleton-Associated Protein of <i>Trypanosoma brucei</i> Linking <i>Tb</i>BILBO1 of the Flagellar Pocket Collar with the Hook Complex |
| title_sort |
bhalin, an essential cytoskeleton-associated protein of <i>trypanosoma brucei</i> linking <i>tb</i>bilbo1 of the flagellar pocket collar with the hook complex |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/af2f51dfb5f1471ba8c85b0ce0261886 |
| work_keys_str_mv |
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