Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles

Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles

Magdalena Stevanović1, Branimir Kovačević2, Jana Petković3, Metka Filipič3, Dragan Uskoković11Institute of Technical Sciences of Serbian Academy of Sciences and Arts, 2Institute of General and Physical Chemistry, Belgrade, Serbia; 3Department of Genetic Toxicology and Cancer Biology, National Instit...

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Autores principales: Stevanović M, Kovačević B, Petković J, Filipič M, Uskoković D
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2011
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/af37aa74487f4bdcae03d82054b1cfb5
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spelling oai:doaj.org-article:af37aa74487f4bdcae03d82054b1cfb52021-12-02T04:59:17ZEffect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles1176-91141178-2013https://doaj.org/article/af37aa74487f4bdcae03d82054b1cfb52011-11-01T00:00:00Zhttp://www.dovepress.com/effect-of-poly-alpha-gamma-l-glutamic-acid-as-a-capping-agent-on-morph-a8664https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Magdalena Stevanović1, Branimir Kovačević2, Jana Petković3, Metka Filipič3, Dragan Uskoković11Institute of Technical Sciences of Serbian Academy of Sciences and Arts, 2Institute of General and Physical Chemistry, Belgrade, Serbia; 3Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, SloveniaAbstract: Highly stable dispersions of nanosized silver particles were synthesized using a straightforward, cost-effective, and ecofriendly method. Nontoxic glucose was utilized as a reducing agent and poly- α, γ, L-glutamic acid (PGA), a naturally occurring anionic polymer, was used as a capping agent to protect the silver nanoparticles from agglomeration and render them biocompatible. Use of ammonia during synthesis was avoided. Our study clearly demonstrates how the concentration of the capping agent plays a major role in determining the dimensions, morphology, and stability, as well as toxicity of a silver colloidal solution. Hence, proper optimization is necessary to develop silver colloids of narrow size distribution. The samples were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurement. MTT assay results indicated good biocompatibility of the PGA-capped silver nanoparticles. Formation of intracellular reactive oxygen species was measured spectrophotometrically using 2,7-dichlorofluorescein diacetate as a fluorescent probe, and it was shown that the PGA-capped silver nanoparticles did not induce intracellular formation of reactive oxygen species.Keywords: silver nanoparticles, poly-α, γ, L-glutamic, green synthesis, morphology, cytotoxicityStevanović MKovačević BPetković JFilipič MUskoković DDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2837-2847 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Stevanović M
Kovačević B
Petković J
Filipič M
Uskoković D
Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
description Magdalena Stevanović1, Branimir Kovačević2, Jana Petković3, Metka Filipič3, Dragan Uskoković11Institute of Technical Sciences of Serbian Academy of Sciences and Arts, 2Institute of General and Physical Chemistry, Belgrade, Serbia; 3Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, SloveniaAbstract: Highly stable dispersions of nanosized silver particles were synthesized using a straightforward, cost-effective, and ecofriendly method. Nontoxic glucose was utilized as a reducing agent and poly- α, γ, L-glutamic acid (PGA), a naturally occurring anionic polymer, was used as a capping agent to protect the silver nanoparticles from agglomeration and render them biocompatible. Use of ammonia during synthesis was avoided. Our study clearly demonstrates how the concentration of the capping agent plays a major role in determining the dimensions, morphology, and stability, as well as toxicity of a silver colloidal solution. Hence, proper optimization is necessary to develop silver colloids of narrow size distribution. The samples were characterized by Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, field-emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurement. MTT assay results indicated good biocompatibility of the PGA-capped silver nanoparticles. Formation of intracellular reactive oxygen species was measured spectrophotometrically using 2,7-dichlorofluorescein diacetate as a fluorescent probe, and it was shown that the PGA-capped silver nanoparticles did not induce intracellular formation of reactive oxygen species.Keywords: silver nanoparticles, poly-α, γ, L-glutamic, green synthesis, morphology, cytotoxicity
format article
author Stevanović M
Kovačević B
Petković J
Filipič M
Uskoković D
author_facet Stevanović M
Kovačević B
Petković J
Filipič M
Uskoković D
author_sort Stevanović M
title Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
title_short Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
title_full Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
title_fullStr Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
title_full_unstemmed Effect of poly-α, γ, L-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
title_sort effect of poly-α, γ, l-glutamic acid as a capping agent on morphology and oxidative stress-dependent toxicity of silver nanoparticles
publisher Dove Medical Press
publishDate 2011
url https://doaj.org/article/af37aa74487f4bdcae03d82054b1cfb5
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