Novel method for rapid toxicity screening of magnetic nanoparticles

Abstract Iron oxide nanoparticles have attracted a great deal of research interest and have been widely used in bioscience and clinical research including as contrast agents for magnetic resonance imaging, hyperthermia and magnetic field assisted radionuclide therapy. It is therefore important to de...

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Autores principales:	A. Erofeev, P. Gorelkin, A. Garanina, A. Alova, M. Efremova, N. Vorobyeva, C. Edwards, Y. Korchev, A. Majouga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/af3b7173dddb4af8b7045f8c1e06cc5d
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spelling	oai:doaj.org-article:af3b7173dddb4af8b7045f8c1e06cc5d2021-12-02T11:40:53ZNovel method for rapid toxicity screening of magnetic nanoparticles10.1038/s41598-018-25852-42045-2322https://doaj.org/article/af3b7173dddb4af8b7045f8c1e06cc5d2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25852-4https://doaj.org/toc/2045-2322Abstract Iron oxide nanoparticles have attracted a great deal of research interest and have been widely used in bioscience and clinical research including as contrast agents for magnetic resonance imaging, hyperthermia and magnetic field assisted radionuclide therapy. It is therefore important to develop methods, which can provide high-throughput screening of biological responses that can predict toxicity. The use of nanoelectrodes for single cell analysis can play a vital role in this process by providing relatively fast, comprehensive, and cost-effective assessment of cellular responses. We have developed a new method for in vitro study of the toxicity of magnetic nanoparticles (NP) based on the measurement of intracellular reactive oxygen species (ROS) by a novel nanoelectrode. Previous studies have suggested that ROS generation is frequently observed with NP toxicity. We have developed a stable probe for measuring intracellular ROS using platinized carbon nanoelectrodes with a cavity on the tip integrated into a micromanipulator on an upright microscope. Our results show a significant difference for intracellular levels of ROS measured in HEK293 and LNCaP cancer cells before and after exposure to 10 nm size iron oxide NP. These results are markedly different from ROS measured after cell incubation with the same concentration of NP using standard methods where no differences have been detected. In summary we have developed a label-free method for assessing nanoparticle toxicity using the rapid (less than 30 minutes) measurement of ROS with a novel nanoelectrode.A. ErofeevP. GorelkinA. GaraninaA. AlovaM. EfremovaN. VorobyevaC. EdwardsY. KorchevA. MajougaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Erofeev P. Gorelkin A. Garanina A. Alova M. Efremova N. Vorobyeva C. Edwards Y. Korchev A. Majouga Novel method for rapid toxicity screening of magnetic nanoparticles
description	Abstract Iron oxide nanoparticles have attracted a great deal of research interest and have been widely used in bioscience and clinical research including as contrast agents for magnetic resonance imaging, hyperthermia and magnetic field assisted radionuclide therapy. It is therefore important to develop methods, which can provide high-throughput screening of biological responses that can predict toxicity. The use of nanoelectrodes for single cell analysis can play a vital role in this process by providing relatively fast, comprehensive, and cost-effective assessment of cellular responses. We have developed a new method for in vitro study of the toxicity of magnetic nanoparticles (NP) based on the measurement of intracellular reactive oxygen species (ROS) by a novel nanoelectrode. Previous studies have suggested that ROS generation is frequently observed with NP toxicity. We have developed a stable probe for measuring intracellular ROS using platinized carbon nanoelectrodes with a cavity on the tip integrated into a micromanipulator on an upright microscope. Our results show a significant difference for intracellular levels of ROS measured in HEK293 and LNCaP cancer cells before and after exposure to 10 nm size iron oxide NP. These results are markedly different from ROS measured after cell incubation with the same concentration of NP using standard methods where no differences have been detected. In summary we have developed a label-free method for assessing nanoparticle toxicity using the rapid (less than 30 minutes) measurement of ROS with a novel nanoelectrode.
format	article
author	A. Erofeev P. Gorelkin A. Garanina A. Alova M. Efremova N. Vorobyeva C. Edwards Y. Korchev A. Majouga
author_facet	A. Erofeev P. Gorelkin A. Garanina A. Alova M. Efremova N. Vorobyeva C. Edwards Y. Korchev A. Majouga
author_sort	A. Erofeev
title	Novel method for rapid toxicity screening of magnetic nanoparticles
title_short	Novel method for rapid toxicity screening of magnetic nanoparticles
title_full	Novel method for rapid toxicity screening of magnetic nanoparticles
title_fullStr	Novel method for rapid toxicity screening of magnetic nanoparticles
title_full_unstemmed	Novel method for rapid toxicity screening of magnetic nanoparticles
title_sort	novel method for rapid toxicity screening of magnetic nanoparticles
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/af3b7173dddb4af8b7045f8c1e06cc5d
work_keys_str_mv	AT aerofeev novelmethodforrapidtoxicityscreeningofmagneticnanoparticles AT pgorelkin novelmethodforrapidtoxicityscreeningofmagneticnanoparticles AT agaranina novelmethodforrapidtoxicityscreeningofmagneticnanoparticles AT aalova novelmethodforrapidtoxicityscreeningofmagneticnanoparticles AT mefremova novelmethodforrapidtoxicityscreeningofmagneticnanoparticles AT nvorobyeva novelmethodforrapidtoxicityscreeningofmagneticnanoparticles AT cedwards novelmethodforrapidtoxicityscreeningofmagneticnanoparticles AT ykorchev novelmethodforrapidtoxicityscreeningofmagneticnanoparticles AT amajouga novelmethodforrapidtoxicityscreeningofmagneticnanoparticles
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Novel method for rapid toxicity screening of magnetic nanoparticles

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