Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.

Centromere protein A (CENP-A) is a histone H3 variant that defines centromeric chromatin and is essential for centromere function. In most eukaryotes, CENP-A-containing chromatin is epigenetically maintained, and centromere identity is inherited from one cell cycle to the next. In the germ line of t...

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Autores principales:	Reinier F Prosée, Joanna M Wenda, Isa Özdemir, Caroline Gabus, Kamila Delaney, Francoise Schwager, Monica Gotta, Florian A Steiner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/af3b9f8ea4064b18a74cd60d2f40da2b
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spelling	oai:doaj.org-article:af3b9f8ea4064b18a74cd60d2f40da2b2021-12-02T19:54:24ZTransgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.1544-91731545-788510.1371/journal.pbio.3000968https://doaj.org/article/af3b9f8ea4064b18a74cd60d2f40da2b2021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.3000968https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Centromere protein A (CENP-A) is a histone H3 variant that defines centromeric chromatin and is essential for centromere function. In most eukaryotes, CENP-A-containing chromatin is epigenetically maintained, and centromere identity is inherited from one cell cycle to the next. In the germ line of the holocentric nematode Caenorhabditis elegans, this inheritance cycle is disrupted. CENP-A is removed at the mitosis-to-meiosis transition and is reestablished on chromatin during diplotene of meiosis I. Here, we show that the N-terminal tail of CENP-A is required for the de novo establishment of centromeres, but then its presence becomes dispensable for centromere maintenance during development. Worms homozygous for a CENP-A tail deletion maintain functional centromeres during development but give rise to inviable offspring because they fail to reestablish centromeres in the maternal germ line. We identify the N-terminal tail of CENP-A as a critical domain for the interaction with the conserved kinetochore protein KNL-2 and argue that this interaction plays an important role in setting centromere identity in the germ line. We conclude that centromere establishment and maintenance are functionally distinct in C. elegans.Reinier F ProséeJoanna M WendaIsa ÖzdemirCaroline GabusKamila DelaneyFrancoise SchwagerMonica GottaFlorian A SteinerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 19, Iss 7, p e3000968 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Reinier F Prosée Joanna M Wenda Isa Özdemir Caroline Gabus Kamila Delaney Francoise Schwager Monica Gotta Florian A Steiner Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.
description	Centromere protein A (CENP-A) is a histone H3 variant that defines centromeric chromatin and is essential for centromere function. In most eukaryotes, CENP-A-containing chromatin is epigenetically maintained, and centromere identity is inherited from one cell cycle to the next. In the germ line of the holocentric nematode Caenorhabditis elegans, this inheritance cycle is disrupted. CENP-A is removed at the mitosis-to-meiosis transition and is reestablished on chromatin during diplotene of meiosis I. Here, we show that the N-terminal tail of CENP-A is required for the de novo establishment of centromeres, but then its presence becomes dispensable for centromere maintenance during development. Worms homozygous for a CENP-A tail deletion maintain functional centromeres during development but give rise to inviable offspring because they fail to reestablish centromeres in the maternal germ line. We identify the N-terminal tail of CENP-A as a critical domain for the interaction with the conserved kinetochore protein KNL-2 and argue that this interaction plays an important role in setting centromere identity in the germ line. We conclude that centromere establishment and maintenance are functionally distinct in C. elegans.
format	article
author	Reinier F Prosée Joanna M Wenda Isa Özdemir Caroline Gabus Kamila Delaney Francoise Schwager Monica Gotta Florian A Steiner
author_facet	Reinier F Prosée Joanna M Wenda Isa Özdemir Caroline Gabus Kamila Delaney Francoise Schwager Monica Gotta Florian A Steiner
author_sort	Reinier F Prosée
title	Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.
title_short	Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.
title_full	Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.
title_fullStr	Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.
title_full_unstemmed	Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.
title_sort	transgenerational inheritance of centromere identity requires the cenp-a n-terminal tail in the c. elegans maternal germ line.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/af3b9f8ea4064b18a74cd60d2f40da2b
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Transgenerational inheritance of centromere identity requires the CENP-A N-terminal tail in the C. elegans maternal germ line.

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