DISTRIBUTION OF <i>MICA</i> ALLELES IN THE RUSSIAN POPULATION

Stress factors, infections, tumor transformation of the cells of organism induce the expression of MICA protein, which is a ligand for the NKG2D receptor of NK and T cells. The interaction of the NKG2D receptor on the surface of the cells of the immune system with MICA results in activation of lymph...

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Autores principales:	L. M. Kanevskiy, M. V. Grechikhina, E. G. Kuzmina, T. Yu. Mushkarina, A. A. Spelkov, M. A. Streltsova, E. I. Kovalenko
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	mhc mica nkg2d genotyping sequencing population analysis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/af471b94f2e847889705eacd3038acd9
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spelling	oai:doaj.org-article:af471b94f2e847889705eacd3038acd92021-11-18T08:03:48ZDISTRIBUTION OF <i>MICA</i> ALLELES IN THE RUSSIAN POPULATION1563-06252313-741X10.15789/1563-0625-2019-5-959-964https://doaj.org/article/af471b94f2e847889705eacd3038acd92019-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1615https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XStress factors, infections, tumor transformation of the cells of organism induce the expression of MICA protein, which is a ligand for the NKG2D receptor of NK and T cells. The interaction of the NKG2D receptor on the surface of the cells of the immune system with MICA results in activation of lymphocytes and elimination of the ligand carrier. The MICA gene has a high level of polymorphism. To date, 87 alleles have been described; their products differ in ability to activate cytotoxic lymphocytes, that can affect the progression of a number of diseases, such as cancer, viral infections, autoimmune diseases. The distribution of MICA alleles in different ethnic groups varies considerably. The analysis of MICA polymorphism in a current ethnos is necessary for revealing the relationships between certain MICA alleles and different diseases. Goal. This work is aimed at studying of the distribution of MICA alleles in Russian population. Materials and methods. Polymorphism of MICA was analyzed according to the procedure proposed by Yizhou Zoe and Peter Stastny. The procedure included: 1) isolation of genomic DNA from whole blood; 2) PCR for amplification of a fragment of the MICA gene; 3) sequencing of the resulting PCR fragments. Analysis of the results of sequencing was carried out using the programs Vector NTI and Chromas Lite. Results. The genotype of the MICA alleles of 119 donors has been determined. Of the 87 MICA alleles described in the literature, 15 were found among the samples studied. The frequencies of MICA alleles were the following: 002 – 19.3%, 004 – 6.7%, 007 – 3.0%, 008 – 35.7%, 009 – 10.1%, 010 – 5.0%, 011 – 3.8%, 012 – 2.1%, 016 – 2.5%, 017 – 3.4% 018 – 5.5%, 019 – 0.4%, 027 – 1.3%, 053 – 0.8%, *068 – 0.4%. The distribution of MICA alleles in Russia was found to be similar to that of European countries. When comparing literary data for different countries of the world, it was found that the differences in the distribution of MICA alleles are expressed mainly between races, and not nations. Conclusions. In this paper, the distribution of MICA alleles in Russian population has been analyzed. It turned out to be very similar to those of other European countries and has a number of significant differences from the ethnoses of the Mongoloid race (Japan, China, Korea). The analysis of the distribution of MICA alleles in the Russian population may be useful for identifying the predisposition of individuals to certain diseases.L. M. KanevskiyM. V. GrechikhinaE. G. KuzminaT. Yu. MushkarinaA. A. SpelkovM. A. StreltsovaE. I. KovalenkoSPb RAACIarticlemhcmicankg2dgenotypingsequencingpopulation analysisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 5, Pp 959-964 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	mhc mica nkg2d genotyping sequencing population analysis Immunologic diseases. Allergy RC581-607
spellingShingle	mhc mica nkg2d genotyping sequencing population analysis Immunologic diseases. Allergy RC581-607 L. M. Kanevskiy M. V. Grechikhina E. G. Kuzmina T. Yu. Mushkarina A. A. Spelkov M. A. Streltsova E. I. Kovalenko DISTRIBUTION OF <i>MICA</i> ALLELES IN THE RUSSIAN POPULATION
description	Stress factors, infections, tumor transformation of the cells of organism induce the expression of MICA protein, which is a ligand for the NKG2D receptor of NK and T cells. The interaction of the NKG2D receptor on the surface of the cells of the immune system with MICA results in activation of lymphocytes and elimination of the ligand carrier. The MICA gene has a high level of polymorphism. To date, 87 alleles have been described; their products differ in ability to activate cytotoxic lymphocytes, that can affect the progression of a number of diseases, such as cancer, viral infections, autoimmune diseases. The distribution of MICA alleles in different ethnic groups varies considerably. The analysis of MICA polymorphism in a current ethnos is necessary for revealing the relationships between certain MICA alleles and different diseases. Goal. This work is aimed at studying of the distribution of MICA alleles in Russian population. Materials and methods. Polymorphism of MICA was analyzed according to the procedure proposed by Yizhou Zoe and Peter Stastny. The procedure included: 1) isolation of genomic DNA from whole blood; 2) PCR for amplification of a fragment of the MICA gene; 3) sequencing of the resulting PCR fragments. Analysis of the results of sequencing was carried out using the programs Vector NTI and Chromas Lite. Results. The genotype of the MICA alleles of 119 donors has been determined. Of the 87 MICA alleles described in the literature, 15 were found among the samples studied. The frequencies of MICA alleles were the following: 002 – 19.3%, 004 – 6.7%, 007 – 3.0%, 008 – 35.7%, 009 – 10.1%, 010 – 5.0%, 011 – 3.8%, 012 – 2.1%, 016 – 2.5%, 017 – 3.4% 018 – 5.5%, 019 – 0.4%, 027 – 1.3%, 053 – 0.8%, *068 – 0.4%. The distribution of MICA alleles in Russia was found to be similar to that of European countries. When comparing literary data for different countries of the world, it was found that the differences in the distribution of MICA alleles are expressed mainly between races, and not nations. Conclusions. In this paper, the distribution of MICA alleles in Russian population has been analyzed. It turned out to be very similar to those of other European countries and has a number of significant differences from the ethnoses of the Mongoloid race (Japan, China, Korea). The analysis of the distribution of MICA alleles in the Russian population may be useful for identifying the predisposition of individuals to certain diseases.
format	article
author	L. M. Kanevskiy M. V. Grechikhina E. G. Kuzmina T. Yu. Mushkarina A. A. Spelkov M. A. Streltsova E. I. Kovalenko
author_facet	L. M. Kanevskiy M. V. Grechikhina E. G. Kuzmina T. Yu. Mushkarina A. A. Spelkov M. A. Streltsova E. I. Kovalenko
author_sort	L. M. Kanevskiy
title	DISTRIBUTION OF <i>MICA</i> ALLELES IN THE RUSSIAN POPULATION
title_short	DISTRIBUTION OF <i>MICA</i> ALLELES IN THE RUSSIAN POPULATION
title_full	DISTRIBUTION OF <i>MICA</i> ALLELES IN THE RUSSIAN POPULATION
title_fullStr	DISTRIBUTION OF <i>MICA</i> ALLELES IN THE RUSSIAN POPULATION
title_full_unstemmed	DISTRIBUTION OF <i>MICA</i> ALLELES IN THE RUSSIAN POPULATION
title_sort	distribution of <i>mica</i> alleles in the russian population
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/af471b94f2e847889705eacd3038acd9
work_keys_str_mv	AT lmkanevskiy distributionofimicaiallelesintherussianpopulation AT mvgrechikhina distributionofimicaiallelesintherussianpopulation AT egkuzmina distributionofimicaiallelesintherussianpopulation AT tyumushkarina distributionofimicaiallelesintherussianpopulation AT aaspelkov distributionofimicaiallelesintherussianpopulation AT mastreltsova distributionofimicaiallelesintherussianpopulation AT eikovalenko distributionofimicaiallelesintherussianpopulation
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DISTRIBUTION OF <i>MICA</i> ALLELES IN THE RUSSIAN POPULATION

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